A5047873191- 3D Printing in Biomedical Research
- Angiogenesis and VEGF in Cancer
- Cellular Mechanics and Interactions
- Additive Manufacturing and 3D Printing Technologies
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Congenital heart defects research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer Cells and Metastasis
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Nanoparticle-Based Drug Delivery
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Cell Adhesion Molecules Research
- Phagocytosis and Immune Regulation
- Blood Coagulation and Thrombosis Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Immune cells in cancer
- Cancer, Hypoxia, and Metabolism
- Venous Thromboembolism Diagnosis and Management
- Zebrafish Biomedical Research Applications
- Caveolin-1 and cellular processes
- Cardiac Ischemia and Reperfusion
Indiana University
2024
Indiana University School of Medicine
2018-2024
Indiana University – Purdue University Indianapolis
2016-2023
Richard L. Roudebush VA Medical Center
2021-2023
Purdue University West Lafayette
2023
Institute for Medical Research
2021
University of Indianapolis
2016-2018
The Ohio State University
2005-2015
Lung Institute
2000-2014
Columbus Oncology and Hematology Associates
2012-2013
Bioprinting is a technology with the prospect to change way many diseases are treated, by replacing damaged tissues live de novo created biosimilar constructs. However, after more than decade of incubation and proofs concept, field still in its infancy. The current stagnation consequence early success: first bioprinters, most those that followed, were modified versions three-dimensional printers used additive manufacturing, redesigned for layer-by-layer dispersion biomaterials. In all...
Homocysteine is a risk factor for the development of atherosclerosis and its thrombotic complications. We have employed an animal model to explore hypothesis that increase in reactive oxygen species subsequent loss nitric oxide bioactivity contribute endothelial dysfunction mild hyperhomocysteinemia. examined function vivo oxidant burden mice heterozygous deletion cystathionine β-synthase (CBS) gene, by studying isolated, precontracted aortic rings mesenteric arterioles situ. CBS–/+...
Abstract —In a transgenic model of ischemic cardiomyopathy in which monocytes are attracted to the myocardium by targeted overexpression monocyte chemoattractant protein-1 (MCP-1), we have observed presence endothelial NO synthase and platelet cell adhesion molecule-1–negative tunnels, occasionally containing blood-derived cells, that probe cardiac tissue. Immunohistochemical data show monocytes/macrophages (MCs/Mphs) drill tunnels using broad-spectrum mouse macrophage metalloelastase....
Background— Oxidant stress has been implicated in the pathogenesis of atherothrombosis and other vascular disorders accompanied by endothelial dysfunction. Glutathione peroxidases (GPx) play an important role cellular defense against oxidant utilizing glutathione (GSH) to reduce lipid hydroperoxides hydrogen peroxide their corresponding alcohols. Cellular GPx (GPx-1) is principal intracellular isoform GPx. We hypothesized that GPx-1 deficiency per se induces dysfunction structural...
Abstract —We studied the association between production of reactive oxygen species, actin organization, and cellular motility. We have used an endothelial cell monolayer–wounding assay to demonstrate that cells at margin wound thus created produced significantly more free radicals than did in distant rows. The rate incorporation monomers into filaments was fastest margin, where heightened detected. tested effect decreasing species on migration polymerization. NADPH inhibitor diphenylene...
Endothelial cells provide an antithrombotic and anti-inflammatory barrier for the normal vessel wall. Dysfunction of endothelial has been shown to promote atherosclerosis, normalization previously dysfunctional can inhibit genesis atheroma. In arteries, are remarkably quiescent. Acceleration turnover rate lead their dysfunction. Apoptosis is a physiological process that contributes homeostasis, by eliminating damaged from However, increased cell mediated through accelerated apoptosis may...
Abstract Adipose tissue is a rich source of multipotent mesenchymal stem-like cells, located in the perivascular niche. Based on their surface markers, these have been assigned to two main categories: CD31−/CD45−/CD34+/CD146− cells (adventitial stromal/stem [ASCs]) and CD31−/CD45−/CD34−/CD146+ (pericytes [PCs]). These populations display heterogeneity unknown significance. We hypothesized that aldehyde dehydrogenase (ALDH) activity, functional marker primitivity, could help better define ASC...
Recently a protocol is established to obtain large quantities of human induced pluripotent stem cells (iPSC)-derived endothelial progenitors, called colony forming (ECFC), and candidate smooth-muscle (SMFC). Here, the suitability for assembling in spheroids, larger 3D cell constructs tested. iPSC-derived ECFC SMFC are labeled with tdTomato eGFP, respectively. Spheroids formed ultra-low adhesive wells, their dynamic proprieties studied by time-lapse microscopy, or confocal microscopy. also...
The small GTPase rac1 controls actin redistribution to membrane ruffles in fibroblasts and other cell types, as well the activation of NADPH oxidase phagocytes. We explored possibility that these two processes could be related. used a replication-deficient adenoviral vector overexpress constitutively active form rac1, racV12, human mouse aortic endothelial cells. show here that, addition ruffle formation, racV12 induced an increase total amount F-actin within Concurrently,...
Linear arrays of cells, or cell columns, have been observed in the extracellular matrix prior to neovascularization, but their nature and significance remains elusive. Based on emerging evidence implicating a role for monocytes macrophages (MC/MPH) vasculogenesis, we hypothesized that MC/MPH also can form linear branched facilitating co-migration spatial arrangement other types. To test this hypothesis, studied distribution effected by chemotactic migration novel vitro vivo models...
Treating cancer patients with chemotherapeutics, such as doxorubicin (Dox), cause dilated cardiomyopathy and congestive heart failure because of oxidative stress. On the other hand, heat shock factor-1 (HSF-1), a transcription factor for proteins (Hsps), is also known to be activated in response However, possible role HSF-1 activation resultant Hsp25 chemotherapeutic-induced has not been investigated. Using wild-type (HSF-1 +/+ ) knock-out −/− mice, we tested hypothesis that plays...
The ability to control the differentiation of adult hematopoietic stem cells (HSCs) would promote development new cell-based therapies treat multiple degenerative diseases. Systemic injection NaIO3 was used ablate retinal pigment epithelial (RPE) layer in C57Bl6 mice and initiate neural degeneration. HSCs infected ex vivo with lentiviral vector expressing RPE-specific gene RPE65 restored a functional RPE layer, typical phenotype including coexpression another marker, CRALBP, photoreceptor...
Overexpression of HER-2 and VEGF plays a key role in the development metastasis several human cancers. Many FDA-approved therapies targeting both (Trastuzumab, Herceptin) (Bevacizumab, Avastin) are expensive, have unacceptable toxicities often associated with resistance. Here, we evaluate dual antitumor effects combining designed particular peptide vaccine mimics. In vitro, phosphorylation antibody-dependent cellular toxicity were used to validate whether HER-2- VEGF-targeting would be...