A5059023381- Extracellular vesicles in disease
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Cell Adhesion Molecules Research
- MXene and MAX Phase Materials
- Bone Tissue Engineering Materials
- Immune Response and Inflammation
- Phagocytosis and Immune Regulation
- Inhalation and Respiratory Drug Delivery
- Wound Healing and Treatments
- Osteoarthritis Treatment and Mechanisms
- Pregnancy and preeclampsia studies
- Nanoplatforms for cancer theranostics
- Thermal properties of materials
University of Maryland, College Park
2016-2022
Johns Hopkins University
2017-2019
University of Maryland, Baltimore
2017
Johns Hopkins Medicine
2017
Based on their identification as physiological nucleic acid carriers in humans and other organisms, extracellular vesicles (EVs) have been explored therapeutic delivery vehicles for DNA, RNA, cargo. However, efficient loading functional of acids remain a challenge, largely because potential sources degradation aggregation. Here, we report that protonation EVs to generate pH gradient across EV membranes can be utilized enhance vesicle cargo, specifically microRNA (miRNA), small interfering...
Abstract We have previously reported that a group of host cellular microRNAs (miRNAs; miR-34a-5p, miR-122-5p, miR-145-5p, miR-146a-5p, miR-210-3p) are released into the blood during sepsis, some which capable inducing complement activation, cytokine production, and leukocyte migration. Extracellular vesicles (EVs) been proposed as vehicles for extracellular miRNA-mediated intercellular communication. However, biological function plasma EVs associated miRNAs in sepsis largely unknown. In this...
One of the leading causes death worldwide is heart failure. Despite advances in treatment and prevention failure, number affected patients continues to increase. We have recently developed 3D-bioprinted biomaterial-free cardiac tissue that has potential improve function. This study aims evaluate vivo regenerative these patches. The patches were generated using 3D-bioprinting technology conjunction with cellular spheroids created from a coculture human-induced pluripotent stem cell-derived...
Extracellular miRNAs (ex-miRNAs) mediate intercellular communication and play a role in diverse physiological pathological processes. Using small RNA sequencing, we identify that are the most abundant species plasma differentially expressed murine human sepsis, such as miR-146a-5p. Exogenous miR-146a-5p, but not its duplex precursor, induces strong immunostimulatory response through newly identified UU-containing motif TLR7 activation, an immunotolerance by rapid IRAK-1 protein degradation...
Abstract Previous studies have demonstrated that transient myocardial ischemia leads to release of cellular nucleic acids such as RNA. Extracellular RNA reportedly plays a pivotal role in inflammation and ischemic injury animals. profiling has identified numerous microRNA (miRNAs), ss–miR-146a-5p, are upregulated plasma following ischemia, certain uridine-rich miRNAs exhibit strong proinflammatory effects immune cells via ssRNA-sensing mechanism. However, the effect extracellular on cardiac...
Ischemic cardiomyopathy poses a significant public health burden due to the irreversible loss of functional cardiac tissue. Alternative treatment strategies include creation three-dimensional (3D) tissues both replace and augment injured native In this study, we utilize net mold-based method create biomaterial-free 3D tissue compare it current methods using biomaterials. Cardiomyocytes, fibroblasts, endothelial cells were combined hanging drop spheroids. For mold patch method, spheroids...
Extracellular vesicles (EVs) have emerged as promising therapeutic entities in part due to their potential regulate multiple signaling pathways target cells. This is derived from the broad array of constituent and/or cargo molecules associated with EVs. Among these, microRNAs (miRNAs) are commonly implicated important and been a wide variety EV-induced biological phenomena. While controlled loading single miRNAs well-documented approach for enhancing EV bioactivity, has not fully leveraged...
Abstract As evidenced by ongoing clinical trials and increased activity in the commercial sector, extracellular vesicle (EV)-based therapies have begun transition from bench to bedside. this progression continues, one critical aspect EV translation is understanding effects of storage transport conditions. Several studies assessed impact on characteristics such as morphology, uptake, component content, but duration temperature functional bioactivity and, especially, loaded cargo are scarcely...
Abstract Extracellular vesicles (EVs) have emerged as promising therapeutic entities in part due to their potential regulate multiple signaling pathways target cells. This is derived from the broad array of constituent and/or cargo molecules associated with EVs. Among these, microRNAs (miRNAs) are commonly implicated important and been a wide variety EV-induced biological phenomena. While controlled loading single miRNAs well-documented approach for enhancing EV bioactivity, has not fully...