A5058157301- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Reproductive Biology and Fertility
- Monoclonal and Polyclonal Antibodies Research
- Opioid Use Disorder Treatment
- Substance Abuse Treatment and Outcomes
- Reproductive tract infections research
- HER2/EGFR in Cancer Research
- Cancer Cells and Metastasis
- Nanoparticle-Based Drug Delivery
- Microfluidic and Bio-sensing Technologies
- T-cell and Retrovirus Studies
- RNA Interference and Gene Delivery
- Parasites and Host Interactions
- Retinoids in leukemia and cellular processes
- Advanced Radiotherapy Techniques
- Pluripotent Stem Cells Research
- Additive Manufacturing and 3D Printing Technologies
- Ultrasound and Hyperthermia Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Radiopharmaceutical Chemistry and Applications
- HIV, Drug Use, Sexual Risk
- Lymphoma Diagnosis and Treatment
- Extracellular vesicles in disease
- Peptidase Inhibition and Analysis
University of Maryland, College Park
2018-2024
Scottish Parliament
2024
University of Maryland, Baltimore
2018
Barts Health NHS Trust
2018
MacroGenics (United States)
2017
Vanderbilt University
1989-2016
Lipscomb University
2016
Brigham and Women's Hospital
2012
Genentech
2001
State University of New York
1999
Cancer immunotherapy that deploys the host's immune system to recognize and attack tumors, is a promising strategy for cancer treatment. However, its efficacy greatly restricted by immunosuppressive (i.e., immunologically cold) tumor microenvironment (TME). Here, we report an in-situ cryo-immune engineering (ICIE) turning TME from "cold" into "hot". In particular, after ICIE treatment, ratio of CD8+ cytotoxic T cells regulatory increased more than 100 times in not only primary tumors with...
Abstract The transmembrane P-glycoprotein (P-gp) pumps that efflux drugs are a major mechanism of cancer drug resistance. They also important in protecting normal tissue cells from poisonous xenobiotics and endogenous metabolites. Here, we report fucoidan-decorated silica-carbon nano-onion (FSCNO) hybrid nanoparticle targets tumor vasculature to specifically release P-gp inhibitor anticancer into cells. targeting capability the is demonstrated using multiple models. Moreover, reveal superior...
An analysis of the cellular distribution, biosynthesis, and structure human T lymphocyte antigen Leu-3(T4) was performed. By using a sensitive ELISA as well FACS analysis, relative quantities from whole cell lysates surfaces six lines were determined. The T-T hybrid line 255.88, monocyte/macrophage U937, proved to be high producers chosen for additional investigation. antigens shown identical by SDS-PAGE. polypeptide 55,000 AMW under reducing conditions, 63,000 nonreducing conditions. In...
Conventional cryopreservation of mammalian cells requires the use toxic organic solvents (e.g., dimethyl sulfoxide) as cryoprotectants. Consequently, cryopreserved must undergo a tedious washing procedure to remove for their further applications in cell-based medicine, and many precious may be lost or killed during procedure. Trehalose has been explored nontoxic alternative traditional However, do not synthesize trehalose express transporters membranes, lack an approach efficient...
The circulating tumor cells (CTCs, the root cause of cancer metastasis and poor prognosis) are very difficult to culture for scale-up in vitro, which has hampered their use research/prognosis patient-specific therapeutic development. Herein, we report a robust electromicrofluidic chip not only efficient capture heterogeneous (EpCAM+ CD44+) CTCs with high purity but also glutathione-controlled gentle release efficiency viability. This is enabled by coating polydimethylsiloxane (PDMS) surface...
Abstract Background Co-use of benzodiazepines and/or ‘z-drugs’ along with opioids is linked to the rise in drug related deaths (DRD) UK. Understanding patterns co-use could inform harm reduction strategies for reducing DRDs. This study explored how people co-use, including dosages, timings, methods administration, use other substances and desired effects sought. Methods Forty-eight semi-structured interviews across Glasgow Scotland (n = 28), Bristol 10) Teesside England individuals who...
We recently have found that the human T cell antigen Leu-2 was specifically released from Leu-2-bearing cells. The preliminary study showed (RLeu-2) HPB-ALL cells composed of a single polypeptide chain 27,000 molecular weight (mol wt), which smaller than subunit homodimeric molecule on surface. In present study, RLeu-2 further characterized and compared with cellular (CLeu-2). Metabolically radiolabeled this had mol wt 27,000. Cell surface radioiodinated were to release radioactive molecules...
Magnetic nanoparticles, especially superparamagnetic nanoparticles (SPIONs), have attracted tremendous attention for various biomedical applications. Facile synthesis and functionalization together with easy control of the size shape SPIONS to customize their unique properties, made it possible develop different types SPIONs tailored diverse functions/applications. More recently, considerable has been paid thermal effect treatment diseases like cancer nanowarming cryopreserved/banked cells,...
We have examined the expression of Leu-4 (T3) antigen on cell surface and in cytoplasm blast cells from 23 patients with T acute lymphoblastic leukemia lymphoma. In majority cases (17), was absent surface; however, 16 these 17 cases, demonstrated cytoplasmic Leu-4. This discordance between also found thymocytes appeared to be restricted Leu-4, that tests other antigens rarely revealed expression. To study further determinant identified by anti-Leu-4 malignant lymphoblasts,...
Human induced pluripotent stem cells (hiPSCs) possess tremendous potential for tissue regeneration and banking hiPSCs by cryopreservation their ready availability is crucial to widespread use. However, contemporary methods hiPSC are associated with both limited cell survival high concentration of toxic cryoprotectants and/or serum. The latter may cause spontaneous differentiation introduce xenogeneic factors, which compromise the quality hiPSCs. Here, sand from nature discovered be capable...
Antibodies directed against the T-cell antigen receptor-T3 complex mimic and lead to cellular changes consistent with activation. When cells of human line Jurkat were stimulated a monoclonal antibody T3, inositol phosphates produced. In addition trisphosphate, which is product phosphatidylinositol bisphosphate cleavage, second polyphosphate was formed. This compound more polar than trisphosphate but less pentakisphosphate. It cochromatographed tetrakisphosphate from ostrich erythrocytes....
The effects of pertussis toxin and cholera on early events T lymphocyte activation were examined in the cell line, Jurkat. Pertussis treatment these cells increased inositol phosphates production led to increases intracellular free calcium concentration. These produced by isolated B (binding) subunit toxin, alone. Inositol resulting from perturbation antigen receptor-CD3 complex MAb was not affected but markedly inhibited toxin. This effect paralleled elevations cAMP content. However,...
Type 1 diabetes is an autoimmune disease in which the immune system attacks insulin-producing beta cells of pancreatic islets. can be treated with islet transplantation; however, patients must administered immunosuppressants to prevent rejection transplanted islets if they are not autologous or engineered protection/isolation. To overcome biological barriers transplantation, encapsulation strategies have been developed and robustly investigated. While need for immunosuppressants, these...
In this study, we examined the temporal antibody response by immunoblotting analysis in tears and sera of three cynomolgus monkeys (Macaca fasicularis) with primary acute Chlamydia trachomatis serovar B conjunctivitis. The objective was to identify chlamydial antigens stimulating during host responses course self-limiting infection rationale that they may be protective antigens. major outer membrane protein (MOMP), lipopolysaccharide (LPS), polypeptides 60 68 kilodaltons (kDa) were...
Abstract Cancer stem cells (CSCs) are rare cancer that postulated to be responsible for relapse and metastasis. However, CSCs difficult isolate poorly understood. Here, a bioinspired approach label‐free isolation culture of CSCs, by microencapsulating one cell in the nanoliter‐scale hydrogel core each prehatching embryo‐like core–shell microcapsule, is reported. Only small percentage individually microencapsulated can proliferate into colony. Gene protein expression analyses indicate high...