A5044563026- 3D Printing in Biomedical Research
- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Pluripotent Stem Cells Research
- Osteoarthritis Treatment and Mechanisms
- Atomic and Subatomic Physics Research
- Age of Information Optimization
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- IoT Networks and Protocols
- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
- Retinoids in leukemia and cellular processes
- Cellular Mechanics and Interactions
- Neuroscience and Neural Engineering
- Reproductive Biology and Fertility
- Microfluidic and Bio-sensing Technologies
- Cancer Genomics and Diagnostics
- Periodontal Regeneration and Treatments
- Extracellular vesicles in disease
- Cancer Research and Treatments
- ATP Synthase and ATPases Research
- S100 Proteins and Annexins
- Photoacoustic and Ultrasonic Imaging
- Advanced biosensing and bioanalysis techniques
Stanford University
2021-2024
Indian Institute of Technology Bombay
2022-2023
SRM Institute of Science and Technology
2023
The Ohio State University
2013-2021
Lung Institute
2013-2018
Central South University
2017
Second Xiangya Hospital of Central South University
2017
Max Healthcare
2017
In-Q-Tel
2014
Multidrug resistance is a major challenge to cancer chemotherapy. The multidrug phenotype associated with the overexpression of adenosine triphosphate (ATP)-driven transmembrane efflux pumps in cells. Here, we report lipid membrane-coated silica-carbon (LSC) hybrid nanoparticle that targets mitochondria through pyruvate, specifically produce reactive oxygen species (ROS) under near-infrared (NIR) laser irradiation. ROS can oxidize NADH into NAD+ reduce amount ATP available for pumps....
Tumor reinitiating cancer stem-like cells are responsible for recurrence associated with conventional chemotherapy. We developed a doxorubicin-encapsulated polymeric nanoparticle surface-decorated chitosan that can specifically target the CD44 receptors of these cells. This system was engineered to release doxorubicin in acidic environments, which occurs when nanoparticles localized tumor microenvironment and they internalized cellular endosomes/lysosomes. design strategy increases...
A novel core–shell microcapsule system is developed in this study to mimic the miniaturized 3D architecture of pre-hatching embryos with an aqueous liquid-like core embryonic cells and a hydrogel-shell zona pellucida. This done by microfabricating non-planar microfluidic flow-focusing device that enables one-step generation microcapsules alginate hydrogel shell liquid from two fluids. Mouse stem (ES) encapsulated are found survive well (>92%). Moreover, ~20 ES can proliferate form single...
Development of high-fidelity three-dimensional (3D) models to recapitulate the tumor microenvironment is essential for studying biology and discovering anticancer drugs. Here we report a method engineer 3D human tumors, by encapsulating cancer cells in core microcapsules with hydrogel shell miniaturized culture obtain avascular microtumors first. The are then used as building blocks assembling endothelial other stromal create macroscale vascularized tumor. Cells engineered can yield...
Abstract Nanoparticles have demonstrated great potential for enhancing drug delivery. However, the low encapsulation efficiency at high drug-to-nanoparticle feeding ratios and minimal loading content in nanoparticle any are major hurdles to their widespread applications. Here we report a robust eukaryotic cell-like hybrid nanoplatform (EukaCell) of theranostic agents (doxorubicin indocyanine green). The EukaCell consists phospholipid membrane, cytoskeleton-like mesoporous silica matrix...
It is difficult to achieve minimally invasive injectable cell delivery while maintaining high retention and animal survival for in vivo stem therapy of myocardial infarction. Here we show that pluripotent aggregates pre-differentiated into the early cardiac lineage encapsulated a biocompatible biodegradable micromatrix, are suitable delivery. This method significantly improves injected cells by more than six-fold compared with conventional practice injecting single cells, effectively...
Cryopreservation of stem cells is important to meet their ever-increasing demand by the burgeoning cell-based medicine. The conventional slow freezing for cell cryopreservation suffers from inevitable injury associated with ice formation and vitrification (i.e., no visible formation) approach emerging as a new strategy cryopreservation. A major challenge intracellular (IIF, lethal event cells) induced devitrification in previously vitrified solution) during warming at cryogenic temperature...
Abstract Organoids are lumen‐containing multicellular structures that recapitulate key features of the organs, and increasingly used in models disease, drug testing, regenerative medicine. Recent work has 3D culture to form organoids from human induced pluripotent stem cells (hiPSCs) reconstituted basement membrane (rBM) matrices. However, rBM matrices offer little control over microenvironment. More generally, role matrix viscoelasticity directing lumen formation remains unknown. Here,...
A novel coaxial electrospray technology is developed to generate microcapsules with a hydrogel shell of alginate and an aqueous liquid core living cells using two fluids in one step. Approximately 50 murine embryonic stem (ES) encapsulated the high viability (92.3 ± 2.9%) can proliferate form single ES cell aggregate 128.9 17.4 μm each microcapsule within 7 days. Quantitative analyses gene protein expression indicate that cultured miniaturized 3D core-shell have significantly higher...
with a collagen-based core and an alginate hydrogel shell for cell tissue culture. With this system, chemical physical properties of extracellular matrix (ECM) in the microenvironment can be controlled to regulate proliferation development cells/tissues under miniaturized three-dimensional (3D)
Molecular mechanisms underlying inflammation-associated breast tumor growth are poorly studied. S100A7, a pro-inflammatory molecule has been shown to enhance cancer and metastasis. However, the S100A7-mediated molecular in enhancing metastasis unclear.Human tissue plasma samples were used analyze expression of cPLA2, PGE2. S100A7-overexpressing or downregulated human metastatic cells evaluate downstream signaling mechanisms. Bi-transgenic mS100a7a15 overexpression, TNBC C3 (1)/Tag...
Drug resistance due to overexpression of membrane transporters in cancer cells and the existence stem (CSCs) is a major hurdle effective safe chemotherapy. Nanoparticles have been explored overcome drug resistance. However, slowly released from nanoparticles can still be efficiently pumped out drug-resistant cells. Here, hybrid nanoparticle phospholipid polymers developed achieve cold-triggered burst release encapsulated drug. With ice cooling below ∼12 °C for both reduced transporter...
Dielectrophoresis (DEP) has been widely explored to separate cells for various applications. However, existing DEP devices are limited by the high cost associated with use of noble metal electrodes, need high-voltage electric field, and/or discontinuous separation (particularly without electrodes). We developed a device liquid which can be used continuously different types or particles based on positive DEP. The is made polydimethylsiloxane (PDMS), and ionic form advantages low easy...
A near-infrared laser-activated "nanobomb" is synthesized using lipid and multiple polymers to break the extracellular intracellular barriers cytosolic delivery of microRNAs. The nanobomb can be used effectively destroy tumors cancer stem-like cells in vitro vivo with minimal side effects. As a service our authors readers, this journal provides supporting information supplied by authors. Such materials are peer reviewed may re-organized for online delivery, but not copy-edited or typeset....
Stem cell therapy holds great potential for treating ischemic diseases. However, contemporary methods local stem delivery suffer from poor survival/retention after injection. We developed a unique multiscale system by encapsulating therapeutic agent-laden nanoparticles in alginate hydrogel microcapsules and further coentrapping the nano-in-micro capsules with cells collagen hydrogel. The exhibits significantly higher mechanical strength stability than pure Moreover, unlike nanoparticles, do...
Abstract Cancer is the second leading cause of mortality globally. Various nanoparticles have been developed to improve efficacy and safety chemotherapy, photothermal therapy, their combination for treating cancer. However, most existing are low in both subcellular precision drug loading content (<≈5%), effect targeted heating organelles on enhancement chemotherapy has not well explored. Here, a hybrid Py@Si‐TH nanoparticle reported first target cancer cells overexpressed with variant...
The anti-inflammatory secretome of mesenchymal stromal cells (MSCs) is lucrative for the treatment osteoarthritis (OA), a disease characterized by low-grade inflammation. However, precise effects MSC on patient-derived OA tissue lacking. To investigate these effects, alginate encapsulated MSCs are co-cultured with cartilage explants 8 days. Proteoglycan distribution in examined Safranin O staining markedly improved when cultured microbeads as compared to control alone. Total sulfated...