A5075621312- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Advanced biosensing and bioanalysis techniques
- Polymer Surface Interaction Studies
- Nanoplatforms for cancer theranostics
- Immune Response and Inflammation
- Advancements in Transdermal Drug Delivery
- Cancer Cells and Metastasis
- CAR-T cell therapy research
- 3D Printing in Biomedical Research
- Monoclonal and Polyclonal Antibodies Research
- Cancer Immunotherapy and Biomarkers
- Antimicrobial Peptides and Activities
- Fatty Acid Research and Health
- Pesticide Exposure and Toxicity
- Dermatology and Skin Diseases
- DNA and Nucleic Acid Chemistry
- Immune cells in cancer
- Nanoparticle-Based Drug Delivery
- Cholinesterase and Neurodegenerative Diseases
- Electrospun Nanofibers in Biomedical Applications
- Cell Image Analysis Techniques
- Extracellular vesicles in disease
University of Maryland, College Park
2016-2025
University of Maryland, Baltimore
2016-2025
VA Maryland Health Care System
2017-2025
United States Department of Veterans Affairs
2016-2024
University of Maryland Marlene and Stewart Greenebaum Comprehensive Cancer Center
2015-2024
Lancaster University
2022-2024
Terasaki Foundation
2024
Institut de Biologie Moléculaire et Cellulaire
2019
Park University
2017-2019
Baltimore VA Medical Center
2016-2019
Recent studies have demonstrated a simple, potentially universal strategy to enhance vaccine potency, via intralymph node (i.LN) injection. To date, intranodal immunization focused on the delivery of unadjuvanted vaccines (e.g., naked DNA, peptide, or protein). We hypothesized that combining i.LN vaccination with controlled release biomaterials permitting sustained dosing molecular adjuvants local tissue microenvironment would further this promising strategy. test idea, we encapsulated...
Whereas CD4 + T cells conventionally mediate antitumor immunity by providing help to CD8 cells, recent clinical studies have implied an important role for cytotoxic in cancer immunity. Using orthotopic melanoma model, we provide a detailed account of antitumoral cell responses and their regulation major histocompatibility complex class II (MHC II) the skin. Intravital imaging revealed prominent interactions with tumor debris-laden MHC host antigen-presenting that accumulated around nests,...
Materials that allow modular, defined assembly of immune signals could support a new generation rationally designed vaccines promote tunable responses. Toward this goal, we have developed the first polyelectrolyte multilayer (PEM) coatings built entirely from signals. These immune-PEMs (iPEMs) are self-assembled on gold nanoparticle templates through stepwise electrostatic interactions between peptide antigen and polyanionic toll-like receptor (TLR) agonists serve as molecular adjuvants....
Many experimental therapies for autoimmune diseases, such as multiple sclerosis (MS), aim to bias T cells toward tolerogenic phenotypes without broad suppression. However, the link between local signal integration in lymph nodes (LNs) and specificity of systemic tolerance is not well understood. We used intra-LN injection polymer particles study a function signals LN microenvironment. In mouse MS model, introduction encapsulated myelin self-antigen regulatory (rapamycin) permanently reversed...
Abstract We report the 12-month follow-up results of a phase 2 clinical Descartes-08 ( NCT04146051 ), BCMA-directed RNA chimeric antigen receptor T-cell (rCAR-T) therapy for myasthenia gravis (MG) given as an outpatient treatment without lymphodepletion. In Phase 2a part study, all 7 participants who received six weekly infusions exhibited clinically meaningful improvement in common MG severity scales (MG Composite, Activities Daily Living, Quantitative scores, and Quality Life 15-revised)...
Materials that permit control over the release of DNA from surfaces topologically complex implantable devices, such as intravascular stents, could contribute to development new approaches localized delivery DNA. We report fabrication ultrathin, multilayered polyelectrolyte films both immobilization and controlled plasmid stainless steel stents. Our approach makes use an aqueous-based, layer-by-layer method for assembly nanostructured thin consisting alternating layers a hydrolytically...
The effect of 16 d intake 300 mg carotenoids/kg diet (beta-carotene (beta C), bixin (BX), lycopene (LY), lutein (LU), canthaxanthin (CX) or astaxanthin (AX) on xenobiotic metabolizing enzymes in the liver, lung, kidney and small intestine male Wistar rats was assessed. A control group received basal (AIN-76) without carotenoids a positive for enzyme induction 3-methylcholanthrene (3-MC) at 666 mg/kg diet. Cytochrome P450 activity assessed using substrates ethoxyresorufin 1A1,...
Recent studies reveal many biomaterial vaccine carriers are able to activate immunostimulatory pathways, even in the absence of other immune signals. How changing properties polymers during biodegradation impact this intrinsic immunogenicity is not well studied, yet information could contribute rational design degradable that help direct response. We use poly(beta-amino esters) (PBAEs) explore as a function degree polymer degradation and form (e.g., soluble, particles). PBAE particles...
Microneedles (MNs) are micron-scale polymeric or metallic structures that offer distinct advantages for vaccines by efficiently targeting skin-resident immune cells, eliminating injection-associated pain, and improving patient compliance. These advantages, along with recent studies showing therapeutic benefits achieved using traditional intradermal injections in human cancer patients, suggest MN delivery might enhance immunotherapies. We recently developed a new class of polyelectrolyte...