A5060750429- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Growth Hormone and Insulin-like Growth Factors
- Single-cell and spatial transcriptomics
- Malaria Research and Control
- vaccines and immunoinformatics approaches
- Bioactive Compounds and Antitumor Agents
- Viral gastroenteritis research and epidemiology
- Phagocytosis and Immune Regulation
- Infectious Diseases and Tuberculosis
- Extracellular vesicles in disease
- Transplantation: Methods and Outcomes
- T-cell and Retrovirus Studies
- Traditional and Medicinal Uses of Annonaceae
- Piperaceae Chemical and Biological Studies
- Fungal Infections and Studies
- Chemokine receptors and signaling
- Ovarian function and disorders
- Insect Pest Control Strategies
- Infective Endocarditis Diagnosis and Management
- Mycobacterium research and diagnosis
- Vector-borne infectious diseases
The University of Melbourne
2020-2025
Peter Doherty Institute
2020-2025
Fundação Oswaldo Cruz
2013-2024
Hospitais da Universidade de Coimbra
1994
Whereas CD4 + T cells conventionally mediate antitumor immunity by providing help to CD8 cells, recent clinical studies have implied an important role for cytotoxic in cancer immunity. Using orthotopic melanoma model, we provide a detailed account of antitumoral cell responses and their regulation major histocompatibility complex class II (MHC II) the skin. Intravital imaging revealed prominent interactions with tumor debris-laden MHC host antigen-presenting that accumulated around nests,...
Abstract Mucosal-associated Invariant T (MAIT) cells are recognized for their antibacterial functions. The protective capacity of MAIT has been demonstrated in murine models local infection, including the lungs. Here we show that during systemic infection mice with Francisella tularensis live vaccine strain results evident cell expansion liver, lungs, kidney and spleen peripheral blood. responding manifest a polarised Th1-like MAIT-1 phenotype, transcription factor cytokine profile, confer...
Naive CD4+ T cells must differentiate in order to orchestrate immunity Plasmodium, yet understanding of their emerging phenotypes, clonality, spatial distributions, and cellular interactions remains incomplete. Here, we observe that splenic polyclonal toward helper 1 (Th1) follicular (Tfh)-like states exhibit rarer phenotypes not elicited among cell receptor (TCR) transgenic counterparts. TCR clones present at higher frequencies Th1 skewing, suggesting variation major histocompatibility...
Abstract Mucosal-associated invariant T (MAIT) cells are important for immune responses against microbial infections. Although known to undergo marked numerical changes with age in humans, our understanding of how MAIT altered during different phases across the human life span is largely unknown. also abundant tissues, study focuses on cell analyses blood. Across span, we show that naive-like umbilical cord blood switch a central/effector memory-like profile sustained into older age. Whereas...
Plasmodium- specific CD4 + T cells differentiate into effector and memory subsets during experimental malaria, via mechanisms that remain incompletely characterised. By mining scRNA-seq data of Plasmodium chabaudi AS infection in mice, we identified two genes previously uncharacterised helper cells, long-tailed unconventional myosin 1f ( Myo1f ) proline-rich13/taxanes-resistance 1 Prr13 / Txr1 ), which were upregulated differentiation. is reported to regulate motility granule exocytosis...
The main control strategy for visceral leishmaniasis in Brazil has been based on the elimination of seropositive dogs, although this is not widely accepted. In context, use a long-lasting protective vaccine against canine (CVL) highly expected. aim work was to determine timeline kinetics cytokine microenvironment derived from circulating leukocytes as supportive immunological biomarkers triggered by Leishmune® vaccine. Cross-sectional kinetic analysis cellular immunity cytokines carried out...
The process for obtaining monoclonal antibodies against a specific antigen is very laborious, involves sophisticated technologies and it not available in most research laboratories. Considering that cytokines remain partially conserved among species during evolution, the search antibody cross-reactivity an important strategy immunological studies veterinary medicine. In this context, amino acid sequence from human canine have demonstrated 49–96 % homology, suggesting high probability of...
Abstract Children in malaria-endemic regions can experience repeated Plasmodium infections over short periods of time. Effects re-infection on multiple co-existing CD4 + T cell subsets remain unresolved. Here, we examine antigen-experienced cells during mice, using scRNA-seq/TCR-seq and spatial transcriptomics. TCR transgenic EM initiate rapid Th1/Tr1 recall responses prior to proliferating, while GC Tfh counterparts are refractory, with CM /Tfh-like exhibiting modest non-proliferative...
Mucosal-associated invariant T (MAIT) cells are known for their rapid effector functions and antibacterial immune protection. Here, we define the plasticity of interferon-γ (IFN-γ)–producing MAIT1 interleukin-17A (IL-17A)–producing MAIT17 cell subsets in vivo. Whereas T-bet + remained stable all experimental settings, after adoptive transfer or acute Legionella Francisella infection, RORγt could undergo phenotypic functional conversion into both MAIT1/17 − cells. This ensured that played a...
Abstract Piperlongumine is an amide alkaloid found in Piperaceae species that shows a broad spectrum of biological properties, including antitumor and antiparasitic activities. Herein, the leishmanicidal effect piperlongumine its derivatives produced by biomimetic model using metalloporphyrins was investigated. The results showed IC50 values promastigote forms Leishmania infantum amazonensis were 7.9 3.3 µM, respectively. value intracellular amastigote form L. 0.4 with selectivity index 25....
The aim of this study was to identify phenotypic and functional biomarkers associated with distinct clinical status leprosy or reactions. included tuberculoid/borderline (BB/BT/T) lepromatous (BL/L) poles as well Type-1 Type-2 reactions along healthy controls (NI). A range peripheral blood innate (neutrophils - NEU monocytes MON) adaptive immunity (CD4+ CD8+ T-cells) were evaluated ex vivo upon in vitro stimuli M. leprae antigen. Data analysis allowed the selection NEUTLR4+ (ex vivo)...
Mucosal-associated invariant T (MAIT) cells are restricted by MR1 and known to protect against bacterial viral infections. Our understanding of the role MAIT in parasitic infections, such as visceral leishmaniasis (VL) caused protozoan parasites Leishmania donovani , is limited. This study showed that response L. infantum human peripheral blood from children with produced TNF IFN-γ an MR1-dependent manner. The overall frequency was inversely correlated alanine aminotransferase levels, a...
Introduction Visceral leishmaniasis (VL) is an important tropical and neglected disease represents a serious global health problem. The initial interaction between the phagocytes parasite crucial to determine pathogen’s capacity initiate infection it shapes subsequent immune response that will develop. While type-1 T-cells induce IL-6, IL-1β, TNF-α, IL-12 production by monocytes/macrophages fight infection, type-2 are associated with regulatory phenotype (IL-10 TGF-β) successful...
Abstract Single naive B cells amplify and diversify their responses when activated by cognate antigen, via Myc-dependent clonal expansion, immunoglobulin class switch recombination (CSR), phenotypic variation, somatic hypermutation (SHM). Whether these mechanisms act combinatorially in vivo to a single complex pathogen remains unclear. Since diversity the antigenic targets, functional classes, production kinetics of parasite-specific antibodies influences immunity malaria, we test here...
Abstract CD4 + T cells orchestrate adaptive immunity to circulating malaria parasites; yet cellular interactions and molecular mechanisms controlling Th1 Tfh differentiation in the spleen remain be fully defined vivo . Here, using a murine model of CD4-dependent immunity, we tested if Slide-seqV2 , spatial transcriptomic method with near single-cell resolution, could determine locations multiple cell subsets potentially interacting partners during infection. Firstly, readily mapped splenic...
AIMS: To evaluate the role of pigment Lawsonia inermis (henna) as a carrier antigens in transcutaneous vaccination mice. METHODS: Mice were immunized transcutaneously into skin abdomen and ear’s with crude antigen Paracoccidioides brasiliensis bovine serum albumin at concentrations 1, 10 50 mg/mL, three times one week intervals presence or absence pigments L. inermis. One after last immunization, animals evaluated for antibodies, interleucin-4 interferon-gamma cytokines, nitric oxide culture...
Abstract Children in malaria-endemic regions can experience multiple Plasmodium infections over a short period of time, with vitro CD4 + T cell recall responses becoming more regulatory increasing age and exposure. This suggests that repeated infection qualitatively changes cells, although the heterogeneity dynamics these await systematic analysis vivo . Here, we examined TCR transgenic PbTII polyclonal cells during re-infection mice, conjunction scRNA-seq/TCR-seq spatial transcriptomics at...