A5027261545- Metabolomics and Mass Spectrometry Studies
- Bioinformatics and Genomic Networks
- Cardiomyopathy and Myosin Studies
- Gene expression and cancer classification
- RNA and protein synthesis mechanisms
- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Viral Infections and Immunology Research
- S100 Proteins and Annexins
- Force Microscopy Techniques and Applications
- Biosensors and Analytical Detection
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- RNA regulation and disease
- Extracellular vesicles in disease
- Cardiovascular Effects of Exercise
- SARS-CoV-2 detection and testing
- Cytomegalovirus and herpesvirus research
- Calcium signaling and nucleotide metabolism
- Calpain Protease Function and Regulation
- Inflammatory mediators and NSAID effects
- Neuroblastoma Research and Treatments
The Ohio State University
2015-2025
Eurofins (United States)
2024
Eurofins (Denmark)
2024
Cancer Genetics (United States)
2022
The Ohio State University Wexner Medical Center
2020-2021
Lung Institute
2016
Molecular mechanisms underlying inflammation-associated breast tumor growth are poorly studied. S100A7, a pro-inflammatory molecule has been shown to enhance cancer and metastasis. However, the S100A7-mediated molecular in enhancing metastasis unclear.Human tissue plasma samples were used analyze expression of cPLA2, PGE2. S100A7-overexpressing or downregulated human metastatic cells evaluate downstream signaling mechanisms. Bi-transgenic mS100a7a15 overexpression, TNBC C3 (1)/Tag...
The contractile response of the heart can be altered by disease-related protein modifications to numerous proteins. By utilizing an IAANS labeled fluorescent troponin C, , we examined effects ten on Ca2+ binding properties complex and reconstituted thin filament. selected are associated with a broad range cardiac diseases: three subtypes familial cardiomyopathies (dilated, hypertrophic restrictive) ischemia-reperfusion injury. Consistent previous studies, majority had no effect isolated...
Background: Medullary thyroid cancer (MTC) is a frequently metastatic tumor of the that develops from malignant transformation C-cells. These tumors most commonly have activating mutations within RET or RAS proto-oncogenes. Germline result in C-cell hyperplasia, and cause MTC pre-disposition disorder, multiple endocrine neoplasia, type 2A (MEN2A). Single-agent therapies for MTC, including vandetanib (VAN) cabozantinib all MTCs selpercatinib (SEL) RET-mutated lead to partial responses but are...
Integration of transcriptomic and metabolomic data improves functional interpretation disease-related phenotypes, facilitates discovery putative metabolite biomarkers gene targets. For this reason, these are increasingly collected in large (> 100 participants) cohorts, thereby driving a need for the development user-friendly open-source methods/tools their integration. Of note, clinical/translational studies typically provide snapshot (e.g. one time point) profiles and, oftentimes, most...
The value of metabolomics in translational research is undeniable, and data are increasingly generated large cohorts. functional interpretation disease-associated metabolites though difficult, the biological mechanisms that underlie cell type or disease-specific profiles oftentimes unknown. To help fully exploit to aid its interpretation, analysis with other complementary omics data, including transcriptomics, helpful. facilitate such analyses at a pathway level, we have developed RaMP...
Control of calcium binding to and dissociation from cardiac troponin C (TnC) is essential healthy muscle contraction/relaxation. There are numerous aberrant post-translational modifications mutations within a plethora contractile, even non-contractile, proteins that appear imbalance this delicate relationship. The direction extent the resulting change in sensitivity thought drive heart toward one type disease or another. number molecular mechanisms may be responsible for altered properties...
Troponin I (TnI) is a major regulator of cardiac muscle contraction and relaxation. During physiological pathological stress, TnI differentially phosphorylated at multiple residues through different signaling pathways to match function demand. The combination these phosphorylations can exhibit an expected or unexpected functional integration, whereby the two are than that predicted from combined each individual phosphorylation alone. We have shown Ser-23/24 Ser-150 integration simultaneously...
Abstract Serum total immunoglobulin E levels (total IgE) capture the state of immune system in relation to allergic sensitization. High are associated with airway obstruction and poor clinical outcomes pediatric asthma. Inconsistent patient response anti-IgE therapies motivates discovery molecular mechanisms underlying serum IgE level differences children To uncover these using complementary metabolomic transcriptomic data, abundance 529 named metabolites expression 22,772 genes were...
Small‐cell lung cancer (SCLC) is an aggressive neuroendocrine subtype of with poor patient prognosis. However, the mechanisms that regulate SCLC progression and metastasis remain undefined. Here, we show expression slit guidance ligand 2 ( SLIT2 ) tumor suppressor gene reduced in tumors relative to adjacent normal tissue. In addition, receptor, roundabout receptor 1 ROBO1 ), upregulated. We find a positive association between Yes1 associated transcriptional regulator YAP1 )‐expressing...
Abstract The Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), also known as 2019 novel coronavirus (2019-nCoV), is a highly infectious RNA virus. A still-debated percentage of patients develop disease (COVID-19) after infection, whose symptoms include fever, cough, shortness breath and fatigue. life-threatening respiratory are experienced by 10-20% symptomatic patients, particularly those with underlying medical conditions that includes diabetes, COPD pregnancy. One the main...
Worldwide, the number of cancer-related deaths continues to increase due ability cancer cells become chemotherapy-resistant and metastasize. For women with ovarian cancer, a staggering 70% will resistant front-line therapy, cisplatin. Although many mechanisms cisplatin resistance have been proposed, key such remain elusive. The RNA binding protein multiple splicing (RBPMS) binds nascent transcripts regulates splicing, transport, localization, stability. Evidence indicates that RBPMS also...
Plants commonly respond to stressors by modulating the expression of a large family calcium binding proteins including isoforms ubiquitous signaling protein calmodulin (CaM). The various plant CaM are thought differentially regulate activity specific target modulate cellular stress responses. mechanism(s) behind differential activation CaMs is unknown. In this study, we used steady-state and stopped-flow fluorescence spectroscopy investigate strategy which two soybean (sCaM1 sCaM4) have...
DICER1 syndrome is a cancer pre-disposition disorder caused by mutations that disrupt the function of in miRNA processing. Studying molecular, cellular and oncogenic effects these can reveal novel mechanisms control cell homeostasis tumor biology. Here, we conduct first analysis pathogenic allele from 3'UTR. We find allele, rs1252940486, abolishes interaction with PUMILIO RNA binding protein 3'UTR, resulting degradation mRNA AUF1. This single mutational event leads to diminished levels,...
IntLIM uncovers phenotype-dependent linear associations between two types of analytes (e.g. genes and metabolites) in a multi-omic dataset, which may reflect chemically or biologically relevant relationships.The new R package includes newly added support for generalized data types, covariate correction, continuous phenotypic measurements, model validation unit testing. analysis uncovered gene-metabolite separate datasets, the run time is improved over baseline functions by multiple orders...
Immunotherapy has improved the prognosis for many melanoma patients; however, our capacity to predict patient responses and understand biological differences between patients who will or not respond is limited. Gene expression profiling of tumors from immunotherapy focused on deriving primarily immune-related signatures; these have shown limited predictive power. Recent studies highlighted role RNA editing in modulating resistance immunotherapy. To evaluate utility activity as a...
Abstract Background Rapid turnaround time for a high-resolution deceased donor human leukocyte antigen (HLA) typing is critical to improve organ transplantation outcomes. Third generation DNA sequencing platforms such as Oxford Nanopore (ONT) offer the opportunity deliver rapid results at single nucleotide level resolution, in particular data that could be denoised computationally. Here we present computational pipeline (up third field) HLA allele following ONT sequencing. Results From R10.4...
Abstract Background: Rapid turnaround time for a high-resolution deceased donor human leukocyte antigen (HLA) typing is critical to improve organ transplantation outcomes. Third generation DNA sequencing platforms such as Oxford Nanopore (ONT) offer the opportunity deliver rapid results at single nucleotide level resolution, in particular data that could be denoised computationally. Here we present computational pipeline (up third field) HLA allele following ONT sequencing. Results: From...
Abstract The Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), also known as 2019 novel coronavirus (2019-nCoV), is a highly infectious RNA virus. A percentage of patients develop disease (COVID-19) after infection, whose symptoms include fever, cough, shortness breath and fatigue. life-threatening respiratory are experienced by 10–20% symptomatic patients, particularly those with underlying medical conditions. One the main challenges in containment COVID-19 identification...
Inactivation of RB is one the hallmarks cancer, however gaps remain in our understanding how RB-loss changes human cells. Here we show that pRB-depletion results cellular reprogramming, quantitatively measured RB-depletion altered transcriptional, proteomic and metabolic output non-tumorigenic RPE1 These profiles identified widespread cell stress response factors previously linked to E2F function. In addition, find a number additional pathways are sensitive not E2F-regulated may represent...