A5023334527- Peptidase Inhibition and Analysis
- Cell Adhesion Molecules Research
- Ocular Surface and Contact Lens
- Tissue Engineering and Regenerative Medicine
- Immunotherapy and Immune Responses
- Corneal Surgery and Treatments
- Immune Response and Inflammation
- Neuropeptides and Animal Physiology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Advanced Glycation End Products research
- Trace Elements in Health
- Immune cells in cancer
- Inflammatory mediators and NSAID effects
- Mesenchymal stem cell research
- Cellular Mechanics and Interactions
- Sphingolipid Metabolism and Signaling
- Protease and Inhibitor Mechanisms
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Signaling Pathways in Disease
- Toxin Mechanisms and Immunotoxins
- Biochemical and Molecular Research
- Chromium effects and bioremediation
- Chemokine receptors and signaling
University of Connecticut
2017-2024
UConn Health
2012-2023
Brown University
2022
R. G. Kar Medical College and Hospital
2013-2016
National Institute of Cholera and Enteric Diseases
2015
Medical College and Hospital, Kolkata
2013
University of Calcutta
2006
Wayne State University
1999
Indian Institute of Chemical Biology
1996-1999
Saccharomyces cerevisiae has two independent transport systems for the removal of arsenite from cytosol. Acr3p is a plasma membrane transporter that confers resistance to arsenite, presumably by extrusion cells. Ycf1p, member ABC superfamily, catalyzes ATP-driven uptake As(III) into vacuole, also producing arsenite. Vacuolar accumulation requires reductant such as glutathione, suggesting substrate glutathione conjugate, As(GS)3. Disruption either ACR3 or YCF1 gene results in sensitivity and...
The RNA-binding protein HuR (also known as ELAV1) binds to the 3′-untranslated region of mRNAs and regulates transcript stability translation. However, in vivo functions are not well understood. Here, we report that murine is essential for life; postnatal global deletion Elavl1 induced atrophy hematopoietic organs, extensive loss intestinal villi, obstructive enterocolitis, lethality within 10 days. Upon deletion, progenitor cells BM, thymus, intestine underwent apoptosis, whereas quiescent...
HuR, also known as Elavl1, is an RNA-binding protein that regulates embryonic development, progenitor cell survival, and stress responses. The role of HuR in angiogenesis not known. Using a myeloid-specific knock-out mouse model (Elavl1Mø KO), we show expression bone marrow-derived macrophages (BMDMs) needed to maintain the genes enriched AU-rich elements U-rich 3′-UTR. In addition, BMDMs from Elavl1Mø KO mice showed alterations several miRNAs. Interestingly, computational analysis suggested...
Dysregulation of the innate immune response underlies numerous pathological conditions. The TLR4 is prototypical sensor infection or injury that orchestrates via sequential activation both cell surface and endocytic signaling pathways trigger distinct downstream consequences. CD14 binds delivers LPS to has been identified as a positive regulator signal transduction. It logical negative regulators this process also exist maintain critical balance required for fighting infection, healing...
Bone regeneration depends on a pool of bone/cartilage stem/progenitor cells and signaling mechanisms regulating their differentiation. Using in vitro approach, we have shown that PDGF through PDGFRβ inhibits BMP2-induced osteogenesis, significantly attenuates expression BMP2 target genes. We evaluated outcomes treatment with two anabolic agents, using different bone healing models. Targeted deletion αSMA osteoprogenitors, led to increased callus mass, resulting improved biomechanical...
Colistin (COL) belongs to the polymyxin group of drugs which possesses a positive charge and interacts with lipopolysaccharide (LPS) Gram-negative bacterial outer membrane. Additionally, it can penetrate cell membrane disrupt integrity phospholipid leading death. Resistance against COL has been reported in Acinetobacter baumannii, bacteria ESKAPE priority pathogens World Health Organization. Multiple pan-drug-resistant strains A. baumannii are rising many medical facilities they becoming...
Although cyclooxygenase (COX)-2 inhibitors (coxibs) are effective in controlling inflammation, pain, and tumorigenesis, their use is limited by the recent revelation of increased adverse cardiovascular events. The mechanistic basis this side effect not well understood. We show that metabolism endocannabinoids endothelial cell COX-2 coupled to prostacyclin (PGI2) synthase (PGIS) activates nuclear receptor peroxisomal proliferator–activated (PPAR) δ, which negatively regulates expression...
CD13 is a large cell surface peptidase expressed on the monocytes and activated endothelial cells that important for homing to resolving damaged tissue at sites of injury. We showed previously cross-linking human monocytic with activating Abs induces strong adhesion in tyrosine kinase- microtubule-dependent manner. In current study, we examined molecular mechanisms underlying these observations vitro vivo. found U937 induced phosphorylation number proteins, including Src, FAK, ERK,...
Abstract Dendritic cell (DC) Ag cross-presentation is generally associated with immune responses to tumors and viral Ags, enhancement of this process a focus tumor vaccine design. In study, we found that the myeloid surface peptidase CD13 highly specifically expressed on subset DCs responsible for cross-presentation, CD8+ murine splenic DCs. vivo studies indicated lack significantly enhanced T soluble OVA Ag, although development, maturation, processing presentation are normal in CD13KO...
Mesenchymal stem cells (MSCs) are multipotent, tissue-resident that can facilitate tissue regeneration and thus, show great promise as potential therapeutic agents. Functional MSCs have been isolated characterized from a wide array of adult tissues universally identified by the shared expression core panel markers. One these markers is multifunctional cell surface peptidase CD13 has shown to be expressed on human murine many tissues. To investigate whether this universal indicates functional...
The cell surface peptidase CD13 coordinates β1 integrin recycling to promote migration.
To determine the role of CD13 as an adhesion molecule in trafficking inflammatory cells to site injury vivo and its function wound healing following myocardial infarction induced by permanent coronary artery occlusion.Seven days post-permanent ligation, hearts from knockout (CD13(KO)) mice showed significant reductions cardiac function, suggesting impaired absence CD13. Mechanistically, CD13(KO) infarcts increase small, endothelial-lined luminal structures, but no perfusion, arguing against...
Summary CD 13/Aminopeptidase N is a transmembrane metalloproteinase that expressed in many tissues where it regulates various cellular functions. In inflammation, 13 on myeloid cells, up‐regulated endothelial cells at sites of inflammation and mediates monocyte/endothelial adhesion by homotypic interactions. animal models the lack alters profiles infiltrating inflammatory ischaemic injury. Here, we found expression enriched specifically pro‐inflammatory subset monocytes, suggesting may...
Sphingosine Kinase-2 (Sphk2) is responsible for the production of bioactive lipid Sphingosine-1 Phosphate, a key regulator tissue repair. Here we address in vivo significance Kinase -2 renal inflammation/fibrosis response to unilateral ureteral obstruction using both genetic and pharmacological strategies. Obstructed kidneys Sphk2-/- mice showed reduced damage diminished levels injury markers TGFβ1 αSMA when compared wild type controls. We found consistently significant increase...
CD13 is a multifunctional cell surface molecule that regulates inflammatory and angiogenic mechanisms in vitro, but its contribution to these processes vivo or potential roles stem biology remains unexplored. We investigated the impact of loss on model ischemic skeletal muscle injury involves angiogenesis, inflammation, mobilization. Consistent with role as an adhesion molecule, lack altered myeloid trafficking injured muscle, resulting cytokine profiles skewed toward prohealing environment....
Chlamydia trachomatis is recognized as one of the most common sexually transmitted pathogen in world. 50-80% infected females are asymptomatic. These untreated women at risk developing chronic sequelae leading to tubal pathology causing infertility. Infertility defined 1 year unprotected intercourse without pregnancy. It may be primary or secondary. Aim : To find out association genital infection with female Materials and Methodology This case control study has been carried collaboration R....
ABSTRACT Host-microbe interactions that facilitate entry into mammalian cells are essential for obligate intracellular bacterial survival and pathogenesis. Anaplasma phagocytophilum is an bacterium invades neutrophils to cause granulocytic anaplasmosis. The invasin-receptor pairs signaling events induce uptake inadequately defined. A. invasion protein A orchestrates via residues 9–21 (AipA ) engaging unknown receptor. Yeast two-hybrid screening suggested AipA binds within C-terminal amino...
Antigen cross presentation is important for effective immune responses to tumors and viral infections.Dendritic cells are professional antigen presenting unique in their ability cross-present exogenous antigens on MHC class I molecules activate specific cytotoxic T cells.This protocol describes by dendritic (DCs) (bone marrow derived DCs splenic DCs) an vitro vivo assay system using soluble ovalbumin protein.