A5034242644- Extracellular vesicles in disease
- Nanoplatforms for cancer theranostics
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- MicroRNA in disease regulation
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Bacillus and Francisella bacterial research
- Photoreceptor and optogenetics research
- Circular RNAs in diseases
- bioluminescence and chemiluminescence research
- Virus-based gene therapy research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microbial Fuel Cells and Bioremediation
- RNA Research and Splicing
- Bacterial biofilms and quorum sensing
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Molecular Communication and Nanonetworks
- Escherichia coli research studies
- Immune cells in cancer
- Microfluidic and Bio-sensing Technologies
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Advanced Biosensing Techniques and Applications
University of Maryland, College Park
2017-2024
Research Institute for Bioscience and Biotechnology
2017
Institute for Bioscience and Biotechnology Research
2017
Abstract The ability to interconvert information between electronic and ionic modalities has transformed our record actuate biological function. Synthetic biology offers the potential expand communication ‘bandwidth’ by using biomolecules providing electrochemical access redox-based cell signals behaviours. While engineered cells have transmitted molecular devices, for bidirectional stands largely untapped. Here we present a simple electrogenetic device that uses redox carry bacterial in...
For therapies targeting diseases of the gastrointestinal tract, we and others envision probiotic bacteria that synthesize excrete biotherapeutics at disease sites. Toward this goal, have engineered commensal E. coli selectively secrete a model biotherapeutic in presence nitric oxide (NO), an intestinal biomarker for Crohn's (CD). This is accomplished by co-expressing pore forming protein TolAIII with biologic, granulocyte macrophage-colony stimulating factor (GM-CSF). We additionally these...
Sepsis is a deadly condition lacking specific treatment despite decades of research. This has prompted the exploration new approaches, with extracellular vesicles (EVs) emerging as focal area. EVs are nanosized, cell-derived particles that transport bioactive components (i.e., proteins, DNA, and RNA) between cells, enabling both normal physiological functions disease progression depending on context. In particular, have been identified critical mediators sepsis pathophysiology. However, also...
Scientists often exploit the motility of peritrichously flagellated bacteria for various applications. A common alteration is modifying frequency mid-movement changes in direction, known as tumbles. Such differences bacterial swimming patterns can prove difficult to quantify, especially those without access high-speed optical equipment. Traditionally, scientists have resorted less accurate techniques, such soft agar plate assays, or been forced invest costly Here, we present TumbleScore,...
Probiotics, whether taken as capsules or consumed in foods, have been regarded safe for human use by regulatory agencies. Being living cells, they serve "tunable" factories the synthesis of a vast array beneficial molecules. The idea reprogramming probiotics to act controllable factories, producing potential therapeutic molecules under user-specified conditions, represents new and powerful concept drug delivery. Probiotics that delivery vehicles pose several challenges, one being targeting...
Adhesion-free bacterial chemotaxis was quantified in static gradients a wide 2D area generated biopolymer membrane-integrated microfluidic platform.
Extracellular vesicles (EVs) have emerged as promising therapeutic entities in part due to their potential regulate multiple signaling pathways target cells. This is derived from the broad array of constituent and/or cargo molecules associated with EVs. Among these, microRNAs (miRNAs) are commonly implicated important and been a wide variety EV-induced biological phenomena. While controlled loading single miRNAs well-documented approach for enhancing EV bioactivity, has not fully leveraged...
Extracellular vesicles (EVs) are physiological vectors for the intercellular transport of a variety molecules. Among these, small RNAs, and especially microRNAs (miRNAs), have been identified as prevalent components, there has thus robust investigation EVs therapeutic miRNAs delivery. However, intrinsic levels EV-associated generally too low to enable efficient effective outcomes. We hypothesized that miRNA localization could be improved by limiting competing interactions occur throughout...
We developed a hybrid synthetic circuit that co-opts the genetic regulation of native bacterial quorum sensing autoinducer-2 and imposes an extra external controller for maintaining tightly controlled gene expression. This dual-input was mathematically modeled and, by design, can be operated in three modes: constitutive mode enables consistent high levels expression; repressed which there is very little background inducible concentrations two signals (arabinose autoinducer-2) determine net...
Abstract Extracellular vesicles (EVs) have emerged as promising therapeutic entities in part due to their potential regulate multiple signaling pathways target cells. This is derived from the broad array of constituent and/or cargo molecules associated with EVs. Among these, microRNAs (miRNAs) are commonly implicated important and been a wide variety EV-induced biological phenomena. While controlled loading single miRNAs well-documented approach for enhancing EV bioactivity, has not fully...