A5037299132- interferon and immune responses
- Bacterial biofilms and quorum sensing
- Advanced biosensing and bioanalysis techniques
- Bacterial Genetics and Biotechnology
- DNA and Nucleic Acid Chemistry
- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Antimicrobial Peptides and Activities
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Biosensors and Analytical Detection
- Click Chemistry and Applications
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Viral Infections and Vectors
- Biochemical and Molecular Research
- X-ray Diffraction in Crystallography
- Chronic Myeloid Leukemia Treatments
- Crystallization and Solubility Studies
- Bacteriophages and microbial interactions
- Microbial Natural Products and Biosynthesis
- Carbohydrate Chemistry and Synthesis
- Cyclopropane Reaction Mechanisms
- Lung Cancer Treatments and Mutations
University of Notre Dame
2025
Purdue University West Lafayette
2015-2024
Purdue University Institute for Cancer Research
2023-2024
National Renewable Energy Laboratory
2024
University of Colorado Boulder
2024
Leibniz-Institute for New Materials
2024
Environmental Energy & Engineering
2024
The University of Adelaide
2024
Imperial College London
2024
Cancer Research Center
2024
Cancer vaccination may be our best and most benign option for preventing or treating metastatic cancer. However, breakthroughs are hampered by immune suppression in the tumor microenvironment. In this study, we analyzed whether cyclic diguanylate (c-di-GMP), a ligand stimulator of interferon genes (STING), could overcome improve against breast Mice with cancer (4T1 model) were therapeutically immunized an attenuated Listeria monocytogenes (LM)-based vaccine, expressing tumor-associated...
ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTMolecular Fluorescence, Phosphorescence, and Chemiluminescence SpectrometrySusmita Das†, Aleeta M. Powe‡, Gary A. Baker§, Bertha Valle⊥, Bilal El-Zahab¶, Herman O. Sintim∥, Mark Lowry#, Sayo Fakayode∇, Matthew E. McCarroll○, Gabor Patonay◆, Min Li□, Robert Strongin#, Maxwell L. Geng⬡, Isiah Warner*†View Author Information† Department of Chemistry, Louisiana State University, Baton Rouge, 70803, United States‡ University Louisville, Kentucky 40208,...
Split G-rich DNA probes can assemble into active peroxidase-mimicking DNAzymes in the presence of bioanalytes such as DNA, thereby providing a simple and cheap means to detect analytes biological samples. A comprehensive study designed reveal salient probe architectural features reaction conditions that facilitate facile reconstitution enzymatically proficient enzymes unveiled these important findings: (a) The loops connect G3-tracts G-quadruplex structure be replaced with stem-loop or...
Interactions of proteins with low-molecular-weight ligands, such as metabolites, cofactors, and allosteric regulators, are important determinants metabolism, gene regulation, cellular homeostasis. Pharmaceuticals often target these interactions to interfere regulatory pathways. We have developed a rapid, precise, high-throughput method for quantitatively measuring protein-ligand without the need purify protein when performed in cells low background activity. This method, differential radial...
Curcumin is a dietary anti-inflammatory and chemopreventive agent consisting of two methoxyphenol rings connected by conjugated heptadienedione chain. unstable at physiological pH rapidly degrades in an autoxidation reaction to major bicyclopentadione product which the 7-carbon chain has undergone oxygenation double cyclization. Early degradation products (but not final bicyclopentadione) mediate topoisomerase poisoning possibly many other activities curcumin, but it known how what are...
Significance Cyclic-di-GMP (c-di-GMP) is a ubiquitous bacterial second messenger that regulates complex behaviors such as biofilm formation. These are changed by altering the intracellular concentration of c-di-GMP. Degradation c-di-GMP occurs two-step process in which one set phosphodiesterases (PDE-As) linearize molecule into 5ʹ-phosphoguanylyl-(3ʹ,5ʹ)-guanosine (pGpG), followed hydrolysis unidentified (PDE-Bs) two GMPs. High levels pGpG inhibit PDE-As, and thus PDE-B activity important...
ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTFluorescence, Phosphorescence, and ChemiluminescenceNoureen Siraj†, Bilal El-Zahab‡, Suzana Hamdan†, Tony E. Karam†, Louis H. Haber†, Min Li§, Sayo O. Fakayode∥, Susmita Das⊥, Bertha Valle⊗, Robert M. Strongin○, Gabor Patonay#, Herman Sintim×, Gary A. Baker$, Aleeta Powe¶, Mark Lowry○, Jan Karolin&, Chris D. Geddes&, Isiah Warner*†View Author Information† Department of Chemistry, Louisiana State University, Baton Rouge, 70803, United States‡...
Aim: Persistent activation of STING pathway is the basis for several autoimmune diseases. activated by cGAMP, which produced cGAS in presence DNA. Results/methodology: HPLC-based medium throughput screening inhibitors identified suramin as a potent inhibitor. Unlike other reported inhibitors, bind to ATP/GTP binding site, displaced bound DNA from cGAS. Addition THP1 cells reduced levels IFN-β mRNA and protein. Suramin did not inhibit lipopolysaccharide- or Pam3CSK4-induced IL-6 expression....
Quorum sensing (QS), a bacterial process that regulates population-scale behavior, is mediated by small signaling molecules, called autoinducers (AIs), are secreted and perceived, modulating "collective" phenotype. Because the autoinducer AI-2 wide variety of species, its "perception" cues behavior. This response
Thermochemical sulfate reduction experiments with simple amino acid and dilute concentrations of reveal significant degrees mass-independent sulfur isotope fractionation. Enrichments up to 13‰ for 33 S are attributed a magnetic effect (MIE) associated the formation thiol-disulfide, ion-radical pairs. Observed 36 depletions in products explained here by classical (mass-dependent) effects mixing processes. The experimental data contrasts strongly multiple trends Archean samples, which exhibit...
Abstract In the last decade, there has been growing interests in studies aimed at delineating strategies used by various nucleic acid enzymes to facilitate catalysis. Insights gained from such would enable design of better DNA/RNA catalysts for applications as biosensing. DNA and RNA have shown be able catalyze myriads reactions, including peroxidation which are catalyzed G‐quadruplexes. this report, we provide data that clarifies how G‐quadruplex peroxidases achieve Firstly, show covalently...
C-di-GMP regulates important processes involved in biofilm formation and virulence factors production several bacteria. Herein we report a simple fluorescent strategy that allows for the detection of c-di-GMP (as low as 320 nM) using Vc2 class I riboswitch domain sensing region spinach reporting module.
There have been many studies on the relationship between nonpathogenic bacteria and human epithelial cells; however, bidirectional effects of secretomes (secreted substances in which there is no direct bacterium-cell contact) yet to be fully investigated. In this study, we use a transwell model explore transcriptomic bacterial secretions from two different Escherichia coli strains colonic cell line HCT-8 using next-generation transcriptome sequencing (RNA-Seq). E. BL21 W3110, while...
Junction probe nucleic acid detection technology allows the amplified sensing of analytes at isothermal conditions. The addition a second dimension to probes permits use cheap commercially available DNA processing enzymes such as restriction endonucleases detect single nucleotide polymorphisms, SNPs.