A5102773517- Immunotherapy and Immune Responses
- interferon and immune responses
- Virus-based gene therapy research
- Leptospirosis research and findings
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Cancer Research and Treatments
- Viral Infections and Vectors
- vaccines and immunoinformatics approaches
- Ubiquitin and proteasome pathways
- Veterinary medicine and infectious diseases
- RNA Interference and Gene Delivery
- Cytokine Signaling Pathways and Interactions
- Immune cells in cancer
- Peptidase Inhibition and Analysis
- Nanoplatforms for cancer theranostics
- Cancer Cells and Metastasis
- Mycobacterium research and diagnosis
- Medical Imaging Techniques and Applications
- T-cell and Retrovirus Studies
- Monoclonal and Polyclonal Antibodies Research
- Microbial Inactivation Methods
- Bartonella species infections research
Albert Einstein College of Medicine
2009-2024
The Bronx Defenders
2015
New York Proton Center
2015
California Pacific Medical Center
2007-2009
The University of Texas Health Science Center at San Antonio
2001-2009
Royal Tropical Institute
1989-2003
International Drug Development
2002
Brigham and Women's Hospital
1999
University of the West
1995
Amsterdam UMC Location University of Amsterdam
1994
Cancer vaccination may be our best and most benign option for preventing or treating metastatic cancer. However, breakthroughs are hampered by immune suppression in the tumor microenvironment. In this study, we analyzed whether cyclic diguanylate (c-di-GMP), a ligand stimulator of interferon genes (STING), could overcome improve against breast Mice with cancer (4T1 model) were therapeutically immunized an attenuated Listeria monocytogenes (LM)-based vaccine, expressing tumor-associated...
SUMMARY: Two sets of primers derived from genomic DNA libraries Leptospira serovars icterohaemorrhagiae (strain RGA) and bim 1051) enabled the amplification by PCR target fragments leptospiral reference strains belonging to all presently described pathogenic species. The icterohaemoirhagiae-derived (G1/G2) L. interrogans, borgpetersenii, weilii, noguchii, santarosai meyeri, whereas bim-derived (B64-I/B64-II) kirschneri. Southern blot sequence analysis revealed inter-species polymorphism...
Abstract The MAGE-A, MAGE-B, and MAGE-C protein families comprise the class-I MAGE/cancer testes antigens, a group of highly homologous proteins whose expression is suppressed in all normal tissues except developing sperm. Aberrant class I MAGE occurs melanomas many other malignancies, have long been recognized as tumor-specific targets; however, their functions largely unknown. Here, we show that suppression induces apoptosis Hs-294T, A375, S91 MAGE-positive melanoma cell lines members...
We tested urine samples from patients at different stages of current leptospirosis and thereafter to determine whether use the PCR for detection leptospires in can be a valuable alternative culturing. The procedure DNA extraction subsequent applied 15 freshly voided proved twice as sensitive Overall, we were able detect approximately 90% (26 29) samples. Urine serum obtained seven patients, before eighth day illness. Although it is generally assumed that leptospiruria starts second week...
Summary Early diagnosis of leptospirosis is important because severe leptospiral infection can run a fulminant course. The polymerase chain reaction (PCR) was evaluated for the detection leptospires in clinical samples from patients with acute infection. Blood and urine 71 were examined by PCR, culture or serology. Samples 44 (62%) positive PCR as compared to 34 (48%) culture. presence demonstrated 13 before development antibodies, well two who seronegative during their illness at autopsy....
Primers for polymerase chain reaction (PCR) were synthesized from clones derived a Leptospira hardjo (type hardjobovis) library. One pair of synthetic oligonucleotide primers was selected further analysis. Under experimental conditions an amplification obtained with DNA interrogans some serovars belonging to serogroup sejroe. However, very little or no observed other this group. No serogroups, bacteria, eucaryotic organisms. Cattle urine, seeded hardjobovis, processed in several ways and...
New therapies are needed that target breast cancer metastases. In previous studies, we have shown vaccination with pcDNA3.1-Mage-b DNA vaccine is effective against the study presented here, further enhanced efficacy of Mage-b through improved delivery using recombinant Listeria monocytogenes (LM). Three overlapping fragments as well complete protein-encoding region been expressed a fusion protein truncated non-cytolytic form listeriolysin O (LLO) in LM. These different strains were...
Abstract Success of cancer vaccination is strongly hampered by immune suppression in the tumor microenvironment ( TME ). Interleukin IL )‐6 particularly and highly produced triple‐negative breast TNBC ) cells, has been considered as an important contributor to . Therefore, we hypothesized that ‐6 reduction may improve efficacy against through improved T‐cell responses. To prove this hypothesis, investigated effect curcumin, inhibitor production, on a attenuated L isteria monocytogenes at ),...
Background Generally, early-stage breast cancer has a good prognosis. However, if it spreads systemically, especially with pulmonary involvement, prospects worsen dramatically. Importantly, tumor-infiltrating T cells contribute to tumor control, particularly intratumoral tissue-resident memory phenotype are associated an improved clinical outcome. Methods Here, we use adenoviral vector vaccine encoding endogenous tumor-associated antigens adjuvanted interleukin-1β induce tumor-specific (TRM)...
Between 1987 and 1991 leptospirosis in 32 Dutch travelers was diagnosed. Infections were acquired predominantly Thailand other Southeast Asian countries. Contact with surface waters could be confirmed all but one case. Fever, headache, myalgia the most common complaints. Signs included conjunctival injection lymphadenopathy 11 patients each, jaundice 8, nuchal rigidity 3; renal function impaired 8. Leptospires isolated from blood or urine of nine patients. Thirty-one developed an antibody...
ABSTRACT The alpha C protein, a protective surface protein of group B streptococci (GBS), is present in most non-type III GBS strains. Conjugate vaccines composed the and type capsular polysaccharide (CPS) might be against infections. In this study, CPS was covalently coupled to full-length, nine-repeat (resulting III-α9r conjugate vaccine) or two-repeat III-α2r by reductive amination. Initial experiments with vaccine showed that it poorly immunogenic mice respect both antigens suboptimally...
Immune suppression in the tumour microenvironment remains a major limitation to successful immunotherapy of cancer. In current study, we analysed whether natural killer T cell-activating glycolipid α-galactosylceramide could overcome immune and improve vaccination against metastatic breast Mice with cancer (4T1 model) were therapeutically treated Listeria monocytogenes-based vaccine expressing tumour-associated antigen Mage-b followed by as separate agents, or complex stably incorporated...
Cancer immunotherapy is one of the most promising and benign therapies against metastatic cancer. However, cancer patients are old elderly react less efficient to vaccines than young adults, due T cell unresponsiveness. Here we present data vaccination in mice with breast (4T1 model). We tested adaptive innate immune responses foreign antigens (Listeria-derived) self-antigens (tumor-associated (TAA)) their contribution elimination metastases at age. Three different protocols were Listeria: a...
Elderly individuals react less efficiently to vaccines than do adults, mainly because of T-cell unresponsiveness. In this study, we analysed whether tumour-associated antigen (TAA)-specific CD8 responses could be induced by vaccination in old mice with metastatic breast cancer. The effect pcDNA-3.1- and Listeria-based vaccines, expressing TAA Mage-b, on Mage-b-specific immune was tested spleens draining lymph nodes (LNs) mild (4TO7cg) aggressive (4T1) syngeneic mouse tumour models at young...
We studied the enhancement of cytolytic activity T3- natural killer cell-derived clones, T3+ T cell activated (AK) and fresh peripheral blood lymphocytes (PBL) by various crude recombinant interferon (r-IFN) as well IL 2 preparations. It was found that IFN-beta had highest cytotoxicity inducing potency compared to or r-IFN-alpha -gamma This blocked anti-IFN-beta antibodies but not anti-IFN-gamma antibodies. also strongly enhances in cloned cells express receptors determined with anti-Tac...
Interleukin-6, a cytokine produced particularly by triple-negative breast cancers, strongly inhibits T cell responses in the tumor microenvironment. Here we tested cryoablation combined with Meriva (a lecithin delivery system of curcumin improved bioavailability) mice metastatic cancer (4T1). Cryoablation involves killing cells through freezing and thawing, resulting recruitment tumor-specific cells, while stimulates reduction IL-6 TME. plus accumulated activated CD8+ to multiple...
Recently, we demonstrated that stimulator of interferon genes (STING) ligand cyclic di-guanylate (c-di-GMP) is an excellent adjuvant in cancer vaccination but also induces immunogenic tumor cell death. Combination both pathways resulted a nearly complete elimination the metastases breast model. This study discussed below.
Breast cancer incidence and mortality increase with age. A better understanding of the biological behavior metastatic nonmetastatic breast tumors in older subjects may help to develop improved therapies. In this study, we used syngeneic (4TO7cg) (64pT) mouse tumor models at three age levels evaluate Various characteristics that are considered be important for effective anti-breast immunotherapy. These included tomor size growth, metastases, vascularization, gene expression tumor-associated...