A5041132113- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Electrospun Nanofibers in Biomedical Applications
- Biomedical Ethics and Regulation
- Cardiovascular Effects of Exercise
- Health Systems, Economic Evaluations, Quality of Life
- 3D Printing in Biomedical Research
- RNA Interference and Gene Delivery
- Pharmaceutical studies and practices
- Nanoparticle-Based Drug Delivery
- Neuroscience and Neural Engineering
- Cardiac electrophysiology and arrhythmias
- Healthcare Policy and Management
- CRISPR and Genetic Engineering
- Delphi Technique in Research
- Cardiac and Coronary Surgery Techniques
- Viral Infections and Immunology Research
- Additive Manufacturing and 3D Printing Technologies
- Biomedical and Engineering Education
- Organ Donation and Transplantation
- Pharmaceutical Economics and Policy
- Academic Writing and Publishing
- Health and Medical Research Impacts
- Cardiac pacing and defibrillation studies
- Advances in Oncology and Radiotherapy
Johns Hopkins University
2015-2022
Johns Hopkins Medicine
2016-2022
Doctors of the World
2020
Howard Hughes Medical Institute
2019
Cancer Genetics (United States)
2019
Yale University
2011-2015
National Institutes of Health Clinical Center
2012-2014
Center for Practical Bioethics
2012
Background: Inflammation is a prominent feature of arrhythmogenic cardiomyopathy (ACM), but whether it contributes to the disease phenotype not known. Methods: To define role inflammation in pathogenesis ACM, we characterized nuclear factor-κB signaling ACM models vitro and vivo cardiac myocytes from patient induced pluripotent stem cells. Results: Activation signaling, indicated by increased expression accumulation phospho-RelA/p65, occurred both an model (expression JUP 2157del2 neonatal...
Amino acid-based core cross-linked star (CCS) polymers (poly(l-lysine)armpoly(l-cystine)core) with peripheral allyl functionalities were synthesized by sequential ring-opening polymerization (ROP) of amino acid N-carboxyanhydrides (NCAs) via the arm-first approach, using N-(trimethylsilyl)allylamine as initiator. Subsequent functionalization a poly(ethylene glycol) (PEG)–folic conjugate thiol–ene click chemistry afforded poly(PEG-b-l-lysine)armpoly(l-cystine)core stars outer PEG coronas...
Abstract Induced pluripotent stem cells (iPSCs) have elicited excitement in both the scientific and ethics communities for their potential to advance basic translational research. They been hailed as an alternative derivation from embryos that provides a virtually unlimited source of research therapeutic applications. However, with iPSCs is ethically complex, uniquely encompassing concerns associated genomics, immortalized cell lines, transplantation, human reproduction, biobanking....
Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a progressive heart condition which causes fibro-fatty myocardial scarring, arrhythmias, and sudden cardiac death. Most cases of ARVC can be linked to pathogenic mutations in the desmosome, but pathophysiology not well understood, particularly early phases when arrhythmias develop prior structural changes. Here, we created novel human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) model from patient with c.2358delA...
Highlights•Decellularized myocardial slices were repopulated with hiPSC-CMs to make EHS•EHS exhibited coordinated contractions and anisotropic electrical conduction•EHS cultured retained contractile function for >200 days•EHS had different sensitivities ion channel drugs than cell monolayersSummaryHuman induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) hold great promise cardiac studies, but their structural functional immaturity precludes use as faithful models of adult...
Introduction: A key obstacle in the creation of engineered cardiac tissues clinically relevant sizes is limited diffusion oxygen and nutrients. Thus, there a need for organized vascularization within three-dimensional (3D) tissue environment. Human induced pluripotent stem cell (hiPSC)-derived early vascular cells (EVCs) have shown to improve organization networks hydrogels. We hypothesize that introduction EVCs into 3D microtissue spheroids will lead increased microvascular formation...
Summary Human somatic cell reprogramming is a leading technology for accelerating disease modeling and drug discovery. Research organizations are sponsoring initiatives to create libraries of induced pluripotent stem (iPSC) lines broad distribution application. Donor informed consent plays critical role in supporting the ethical conduct iPSC research. To date, our have focused on considerations collection intended specifically derivation distribution. This article considers how cells...
Genetic heart diseases such as arrhythmogenic cardiomyopathy (AC), a common genetic cause of sudden cardiac death, can be modeled using patient-specific induced pluripotent stem cell-derived myocytes (CMs). However, it is important to culture these cells in multicellular syncytium with exposure surrounding matrix cues create more accurate and robust models the disease due importance cell-cell cell-matrix interactions. The engineered slice, constructed by seeding CMs on intact decellularized...
A recent study demonstrated, in a mouse model one lab, that bexarotene (FDA-approved for use non-Hodgkin's lymphoma) is effective against Alzheimer's disease. What should physicians do if patients with disease begin seeking off-label prescriptions?
A "right to withdraw" from research participation is an important mechanism for ensuring voluntariness and respecting participant autonomy throughout a study. This right often expressed as absolute, dictating that can withdraw at any time reason. However, it more challenging define when the interactions between researcher have ended but continuing use biospecimens provided by participant. issue becoming particularly complicated researchers are generating induced pluripotent stem cell (iPSC)...