A5078003343- CRISPR and Genetic Engineering
- Reproductive Biology and Fertility
- Muscle Physiology and Disorders
- Selenium in Biological Systems
- Animal Genetics and Reproduction
- Immune Response and Inflammation
- Sperm and Testicular Function
- Neurogenetic and Muscular Disorders Research
- Salmonella and Campylobacter epidemiology
- Vibrio bacteria research studies
- Animal Nutrition and Physiology
- Aquaculture disease management and microbiota
- Pluripotent Stem Cells Research
- Genetics, Aging, and Longevity in Model Organisms
- Renal and related cancers
- Gut microbiota and health
- Epigenetics and DNA Methylation
- Circadian rhythm and melatonin
- Trace Elements in Health
- Animal Disease Management and Epidemiology
- Antimicrobial Resistance in Staphylococcus
- Vector-Borne Animal Diseases
- Genetics and Neurodevelopmental Disorders
- Moringa oleifera research and applications
- Viral Infections and Immunology Research
China Agricultural University
2015-2024
Ministry of Agriculture and Rural Affairs
2019-2024
Abstract: Two‐cell embryos of mouse were vitrified by the open‐pulled straw (OPS) method. The warmed and introduced into M16 medium for culture that contains melatonin at different concentrations (10 −3 , 10 −5 −7 −9 −11 m ). This process caused reactive oxygen species (ROS) formation jeopardized development embryos. Melatonin, concentrations, significantly suppresses ROS production promotes embryonic in compared with untreated ones. mechanistic studies indicated beneficial effects on 2‐cell...
Goat is an important livestock worldwide, which plays indispensable role in human life by providing meat, milk, fiber, and pelts. Despite recent significant advances microbiome studies, a comprehensive survey on the goat microbiomes covering gastrointestinal tract (GIT) sites, developmental stages, feeding styles, geographical factors still unavailable. Here, we surveyed its multi-kingdom microbial communities using 497 samples from ten sites along GIT.We reconstructed catalog (GMMC)...
The present study aimed to investigate the effect of melatonin (MT) supplementation on in vitro maturation vitrified mouse germinal vesicle (GV) oocytes. fresh oocytes were randomly divided into three groups: untreated (control), or by open-pulled straw method without (vitrification group) with MT + group). After warming, cultured vitro, then reactive oxygen species (ROS) and glutathione (GSH) levels, mitochondrial membrane potential, ATP spindle morphology, mRNA expression assembly...
Abstract Melatonin as a potent antioxidant exhibits important nutritional and medicinal values. To produce melatonin‐enriched milk will benefit the consumers. In this study, sheep bioreactor which generates has been successfully developed by technology that combined CRISPR /Cas9 system microinjection. The AANAT ASMT were cloned from pineal gland of Dorper ( Ovis aries ). in vitro studies found transferred to mammary epithelial cell lines significantly increased melatonin production culture...
Internal egg and eggshell quality are often deteriorated in aging laying hens, which causes huge economic losses the poultry industry. Selenium yeast (SY), as an organic food additive, is utilized to enhance performance quality. To extend production cycle, effects of selenium supplementation on quality, plasma antioxidants deposition aged hens were evaluated. In this study, five hundred twenty-five 76-week-old Jing Hong fed a selenium-deficient (SD) diet for 6 weeks. After Se depletion,...
Sheep are an important source of fiber production. Fibroblast growth factor 5 (FGF5) is a dominant inhibitor length the anagen phase hair cycle. Knockout or silencing gene results in wooly coat mice, donkeys, dogs, and rabbits. In sheep breeding, wool one most quality traits. However, traditional breeding cannot accurately efficiently mediate advanced genotype into genome. this study, we generated 3 knockout via 1-step clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9...
This study was conducted to investigate the effect of vitrification on dynamics global transcriptome in bovine germinal vesicle (GV) oocytes and their vitro-derived metaphase II (MII) oocytes. The GV were vitrified using open-pulled straw method. After warming, resulting MII-stage cultured vitro for 2 h 24 respectively then collected. fresh MII used as controls. Then, each pool (fresh GV, n = 3; 4; MII, 1 derived from 2) different stages mRNA sequencing. results showed that maturation rates...
Female reproductive (ovarian) aging is distinctively characterized by a markedly reduced function due to remarkable decline in quality and quantity of follicles oocytes. Selenium (Se) has been implicated playing many important biological roles male fertility reproduction; however, its potential female reproduction, particularly subjects, remain poorly elucidated. Therefore, the current study we used murine model elucidated how different Se-levels might affect efficiency females. Our results...
Abstract Background This study investigated the effect of melatonin (MT) on cell cycle (G1/S/G2/M) parthenogenetic zygotes developed from vitrified-warmed mouse metaphase II (MII) oocytes and elucidated potential mechanism MT action in first cleavage embryos. Results After vitrification warming, were parthenogenetically activated (PA) vitro cultured (IVC). Then spindle morphology chromosome segregation oocytes, maternal mRNA levels genes including Miss, Doc1r, Setd2 Ythdf2 pronuclear...
This study aimed to investigate the effect of melatonin on cell cycle parthenogenetic embryos derived from vitrified mouse metaphase II (MII) oocytes. Fresh oocytes were randomly allocated into three groups: untreated (control), or by open-pulled straw method without (Vitrification group) with (MT) supplementation + MT group). After warming, parthenogenetically activated and cultured in vitro, then percentage G1/S phase, levels reactive oxygen species (ROS) glutathione (GSH), mRNA expression...
Hypertrophy and fiber transformation are two prominent features of postnatal skeletal muscle development. However, the role epigenetic modifications is less understood. ATAC-seq, whole genome bisulfite sequencing, RNA-seq were applied to investigate dynamics in Hu sheep at 3 days, months, 6 12 months after birth. All 6865 differentially expressed genes assigned into three distinct tendencies, highlighting balanced protein synthesis, accumulated immune activities, restrained cell division We...
With a broad range of hosts, Salmonella enterica serovar Typhimurium (S. Typhimurium) is the major cause gastroenteritis in human beings and systemic disease susceptible mice strains. However, different S. strains differ regard to virulence host adaptation. Here, C57BL/6 were infected, respectively, with SL1344 (calf), CVCC541 (chicken) CMCC50115 (mutton) determine their immune responses. It was found that less infection by strains, lower lethality decreased bacterial burden liver spleen....
Genetic modification offers alternative strategies to traditional animal breeding. However, the food safety of genetically modified (GM) animals has attracted increasing levels concern. In this study, we produced GM sheep overexpressing TLR4, and transgene-positive offsprings (F1) were confirmed using polymerase chain reaction (PCR) Southern blot. The expression TLR4 was 2.5-fold compared with that wild-type (WT) samples. During 90-day Sprague-Dawley rats fed three different dietary...
Staphylococcus aureus infections pose a potential threat to livestock production and public health. A novel strategy is needed control S. due its adaptive evolution antibiotics. Autophagy plays key role in degrading bacteria for innate immune cells. In order promote clearance via Toll-like receptor (TLR)-induced autophagy pathway, the domain fusion TLR2-4 with extracellular of TLR2, specific recognizing , transmembrane intracellular domains TLR4 assembled, then goat expressing generated....
Abstract Foot and mouth disease, which is induced by the foot disease virus (FMDV), takes its toll on cloven-hoofed domestic animals. The VP1 gene in FMDV genome encodes viral capsid, a vital element for replication. Sleeping Beauty (SB) an active DNA-transposon system responsible genetic transformation insertional mutagenesis vertebrates. In this study, conserved -shRNA specifically targets ovine FMDV- was constructed combined with SB transposase transposon. Then, they were microinjected...