A5023833325- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Genetic and phenotypic traits in livestock
- Pluripotent Stem Cells Research
- Cancer-related molecular mechanisms research
- Probiotics and Fermented Foods
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Gut microbiota and health
- Meat and Animal Product Quality
- Fatty Acid Research and Health
- Protein Hydrolysis and Bioactive Peptides
- Ruminant Nutrition and Digestive Physiology
- Milk Quality and Mastitis in Dairy Cows
- Genetic Mapping and Diversity in Plants and Animals
- Viral gastroenteritis research and epidemiology
- Epigenetics and DNA Methylation
- Renal and related cancers
- Sperm and Testicular Function
- Muscle Physiology and Disorders
- Virus-based gene therapy research
- melanin and skin pigmentation
- RNA Research and Splicing
- Livestock Farming and Management
- Genomics and Phylogenetic Studies
Guangxi University
2015-2024
Foshan University
2022-2024
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2014-2024
Agricultural Genomics Institute at Shenzhen
2020
Chinese Academy of Agricultural Sciences
2020
Bridge University
2019-2020
Cloning of buffalos (Bubalus bubalis) through nuclear transfer is a potential alternative approach in genetic improvement buffalos. However, to our knowledge, cloned offspring derived from embryonic, fetal, or somatic cells have not yet been reported. Thus, factors affecting the buffalo were examined, and possibility cloning was explored present study. Treatment fibroblasts granulosa with aphidicolin plus serum starvation resulted more being arrested at G0/G1 phase, proportion DNA...
Abstract Buffalo is an important livestock species. Here, we present a comprehensive metagenomic survey of the microbial communities along buffalo digestive tract. We analysed 695 samples covering eight different sites in three compartments (four-chambered stomach, intestine, and rectum). mapped ~85% raw sequence reads to 4,960 strain-level metagenome-assembled genomes (MAGs) 3,255 species-level MAGs, 90% which appear correspond new In addition, annotated over 5.8 million nonredundant...
Abstract Objective Polycystic ovary syndrome (PCOS) is characterized by follicular dysplasia. An insufficient glycolysis-derived energy supply of granulosa cells (GCs) an important cause dysplasia in PCOS. Follicular fluid (FF) exosomal microRNAs (miRNAs) have been proven to regulate the function GCs. In this study, exosomes extracted from clinical FF samples were used for transcriptome sequencing (RNA-seq) analysis, and a human ovarian granulocyte tumour cell line (KGN cells) was vitro...
Domesticated buffaloes have been integral to rice-paddy agro-ecosystems for millennia, yet relatively little is known about the buffalo genomics. Here, we sequenced and assembled reference genomes both swamp river re-sequenced 230 individuals (132 98 buffaloes) sampled from across Asia Europe. Beyond many actionable insights that our study revealed domestication, basic physiology breeding of buffalo, made striking discovery divergent domestication traits between can be explained with recent...
Goat is an important livestock worldwide, which plays indispensable role in human life by providing meat, milk, fiber, and pelts. Despite recent significant advances microbiome studies, a comprehensive survey on the goat microbiomes covering gastrointestinal tract (GIT) sites, developmental stages, feeding styles, geographical factors still unavailable. Here, we surveyed its multi-kingdom microbial communities using 497 samples from ten sites along GIT.We reconstructed catalog (GMMC)...
Rotavirus is the leading cause of severe dehydrating diarrhea in young children and inner capsid protein VP6 a potential vaccine candidate that can induce cross-protective immune responses against different strains. The use ferritin nanoparticles as scaffold antigen improve immunogenicity subunit vaccines provide broader protection. We here present non-live self-assemble recombinant rotavirus VP6-ferritin (rVP6-ferritin) nanoparticle vaccine.The rVP6-ferritin were expressed E. coli...
Consumers have shown more and interest in high-quality healthy dairy products buffalo milk is commercially viable than other milks producing superior due to its higher contents of fat, crude protein, total solids. Metabolomics one the most powerful strategies molecular mechanism research however, little study has been focused on metabolites different species. Therefore, aim this was explore underlying fatty synthesis candidate biomarkers by analyzing metabolomic profiles. Milk three groups...
Buffalo meat consist good qualitative characteristics as it contains 'thined tender' which is favourable for cardavascular system. However, the regulatory mechanisms of long non-coding RNA (lncRNA), differences in quality are not well known. The chemical-physical parameters revealed tthe muscle buffalo that can be equivalent cattle, but there significant shearing force and fiber structure. Then we examined lncRNA expression profiles cattle skeletal provide first insights into their potential...
This study aimed to reveal the effects and regulatory mechanism of dietary NDF on performance pigs by multi-omics analysis. Results showed that 16 % significantly improved meat quality, increased flavor amino acid content, reduced backfat thickness feed-to-gain ratio. 16S rDNA sequencing abundance Akkermansia, Lachnoclostridium, Ruminococcus. Transcript analysis genes related muscle development lipid metabolism were modified. Metabonomic fatty metabolites. Correlation suggested treatment may...
Milking methods have significant impacts on the microbiological composition, which could affect quality of raw buffalo milk. Hence, current study was conducted impact milking microorganisms in tank milk from 15 farms Guangxi, China. The were divided into two groups based method: mechanical (MM, n = 6) and hand (HM, 9). Somatic cell counts, bacterial counts nutrients samples analyzed. comparison analyzed using metagenomic sequencing to detect any differences between groups. There no...
Abstract Background Buffalo milk is considered as a highly nutritious food owing to its higher contents of fatty acids (FA) and rich nutrient profile. Higher fat buffalo make it suitable for processing develop various healthy products. Moreover, contains more unsaturated FAs (UFA) such oleic linolenic acid, which are important from the human health point view their desirable physiological effects. However, inadequate information available about chemical composition mechanism FA synthesis in...
During triacylglycerol synthesis, the acylglycerol-3-phosphate acyltransferase (AGPAT) family catalyzes conversion of lysophosphatidic acid to phosphatidic and acylation sn-2 fatty acids. However, catalytic activity different AGPAT members is different. Therefore, this study aimed investigate mechanism through which AGPATs affect efficiency TAG synthesis composition. The conservation amino sequences protein domains was analyzed, functions AGPAT1, AGPAT3, AGPAT4 genes in buffalo mammary...
Abstract Cattle and the draught force provided by its skeletal muscle have been integral to agro‐ecosystems of agricultural civilization for millennia. However, relatively little is known about cattle functional genomics (including protein coding genes, non‐coding RNA, etc.). Circular RNAs (circRNAs), as a new class RNAs, can be effectively translated into detectable peptides, which enlightened us on importance circRNAs in physiology function. Here, RNA‐seq, Ribosome profiling (Ribo‐seq),...
Fasciola gigantica and hepatica are causative pathogens of fascioliasis , with the widest latitudinal, longitudinal, altitudinal distribution; however, among parasites, they have largest sequenced genomes, hindering genomic research. In present study, we used various sequencing assembly technologies to generate a new high-quality reference genome. We improved integration gene structure prediction, identified two independent transposable element expansion events contributing (1) speciation...
Abstract Background Leeches have been used in traditional Chinese medicine since prehistoric times to treat a spectrum of ailments, but very little is known about their physiological, genetic, and evolutionary characteristics. Findings We sequenced assembled chromosome-level genomes 3 leech species (bloodsucking Hirudo nipponia Hirudinaria manillensis nonbloodsucking Whitmania pigra). The dynamic population histories genome-wide expression patterns the 2 bloodsucking were found be similar. A...
The buffalo is an important domestic animal globally, providing milk, meat, and labor to more than 2 billion people in 67 countries. rumen microorganisms of buffaloes play indispensable role enabling the healthy functionality digestive function organisms. Currently, there a lack clarity regarding differences composition among at different growth stages.