A5033498671- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- Sperm and Testicular Function
- Tissue Engineering and Regenerative Medicine
- Epigenetics and DNA Methylation
- Weed Control and Herbicide Applications
- Genetic and phenotypic traits in livestock
- Prenatal Screening and Diagnostics
- melanin and skin pigmentation
- Cancer-related molecular mechanisms research
- Genomics and Chromatin Dynamics
- Aquaculture disease management and microbiota
- Occupational and environmental lung diseases
- Dyeing and Modifying Textile Fibers
- Smoking Behavior and Cessation
- Molecular Biology Techniques and Applications
- Seed Germination and Physiology
- MicroRNA in disease regulation
- Plant-Microbe Interactions and Immunity
- Myxozoan Parasites in Aquatic Species
- Gastric Cancer Management and Outcomes
- Plant and Fungal Interactions Research
Guangxi University
2016-2025
Heilongjiang Academy of Sciences
2023-2024
Institute of Plant Protection
2023-2024
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2013-2024
Hunan Normal University
2003-2024
Heilongjiang Provincial Academy of Agricultural Sciences
2024
University of California, Los Angeles
2024
Zhejiang Cancer Hospital
2022
Nanjing Medical University
2015-2020
Nanjing Maternity and Child Health Care Hospital
2018-2020
Domesticated buffaloes have been integral to rice-paddy agro-ecosystems for millennia, yet relatively little is known about the buffalo genomics. Here, we sequenced and assembled reference genomes both swamp river re-sequenced 230 individuals (132 98 buffaloes) sampled from across Asia Europe. Beyond many actionable insights that our study revealed domestication, basic physiology breeding of buffalo, made striking discovery divergent domestication traits between can be explained with recent...
To evaluate comprehensively, using chromosomal microarray analysis (CMA) and exome sequencing (ES), the prevalence of abnormalities sequence variants in unselected fetuses with congenital heart defect (CHD) to potential diagnostic yields CMA ES for different CHD subgroups.This was a study 360 singleton detected by echocardiography, referred our department genetic testing between February 2018 December 2019. We performed CMA, as routine test aneuploidy copy number variations (CNV), then,...
Abstract The growth and maturation of Sertoli cells (SCs) are important part testicular development miRNAs considered as novel regulatory molecules involved in it. Here we systematically screened analyzed the key participating black goat, well identified underlying mechanism candidate mRNAs that contributed to spermatogenesis. miRNA transcriptional profiles tissues at 3 days old (D3), 30 (D30), 90 (D90), 180 (D180) were constructed comparative analyzed. Results showed most differentially...
Ectopically, expression of defined factors could reprogram mammalian somatic cells into induced pluripotent stem (iPSCs), which initiates a new strategy to obtain cell lines. Attempts have been made generate buffalo by culturing primary germ or inner mass, but the efficiency is extremely low. Here, we report successful method fetal fibroblasts (BFFs) [buffalo (biPSCs)] transduction (Oct4, Sox2, Klf4, and c-Myc) using retroviral vectors. The established biPSCs displayed typical morphological...
Buffalo meat consist good qualitative characteristics as it contains 'thined tender' which is favourable for cardavascular system. However, the regulatory mechanisms of long non-coding RNA (lncRNA), differences in quality are not well known. The chemical-physical parameters revealed tthe muscle buffalo that can be equivalent cattle, but there significant shearing force and fiber structure. Then we examined lncRNA expression profiles cattle skeletal provide first insights into their potential...
Copy Number Variations (CNVs) are an important genetic cause of a number neurodevelopmental disorders (NDs). However, the association between CNVs and development prognosis fetal isolated mild ventriculomegaly (IMV) is unclear.To investigate possible associations IMV.This retrospective study recruited 154 subjects with ultrasound-confirmed IMV 190 in control cohort who underwent high-risk prenatal serum screening program. The exclusion criteria included fetus G-banding chromosomal...
Abstract The possibility of producing transgenic cloned buffalos by nuclear transfer fetal fibroblasts expressing enhanced green fluorescent protein (EGFP) was explored in this study. When buffalo (BFFs) isolated from a male fetus were transfected with pEGFP-N1 ( EGFP is driven CMV and Neo SV-40) means electroporation, Lipofectamine-LTX X-tremeGENE, the transfection efficiency electroporation (35.5%) higher than (11.7%) X-tremeGENE (25.4%, P < 0.05). BFFs more embryos pEGFP-IRES-Neo are...
A colored phenotype is an important feature of ornamental fish.In mammals, microphthalmia-associated transcription factor (MITF) was found to regulate the development melanocytes.In this study, mitfa cDNA first cloned from Japanese (Koi) carp (Cyprinus carpio L.), freshwater fish.The full-length gene contains 1634 bp, coding for 412 amino acids in Koi.The identity degree acid sequences between Koi and zebrafish 92.9%.We tested expression several varieties using reverse...
The present study was undertaken to examine the effect of Scriptaid treatment on histone acetylation, DNA methylation, expression genes related and development buffalo somatic cell nuclear transfer (SCNT) embryos. Treatment SCNT embryos with 500 nM for 24 h resulted in a significant increase blastocyst formation rate (28.2% vs. 13.6%, p<0.05). Meanwhile, also higher acetylation levels H3K18 lower methylation global at stage, which similar fertilized counterparts. CBP, p300, HAT1, Dnmt1,...
Abstract Somatic cell nuclear transfer (SCNT) holds vast potential in agriculture. However, its applications are still limited by low efficiency. Histone 3 lysine 9 trimethylation (H3K9me3) was identified as an epigenetic barrier for this. demethylase KDM4D could regulate the level of H3K9me3. effects on buffalo SCNT embryos unclear. Thus, we performed this study to explore and underlying mechanism embryos. The results revealed that compared with IVF embryos, expression significantly lower...
To investigate the contributions and underlying molecular mechanisms of annexin A5 toward silica-induced pulmonary fibrosis.Male C57BL/6 mice were randomly divided into three groups instilled intratracheally with silica, saline, or air. Mice euthanized at 3, 7, 14, 28 days following treatment. Annexin levels in serum lung tissues detected by enzyme-linked immunosorbant assay (ELISA) assays Western blots. The association fibrosis was further investigated macrophage cell line, RAW264.7....
Contents The DNA methylation of imprinted genes is an important way to regulate epigenetic reprogramming donor cells in somatic cell nuclear transfer ( SCNT ). However, the effects sexual distinction on cloned animals have seldom been reported. In this study, we analysed status three Xist , IGF 2 and H19 ) from liveborn buffaloes (L group, female male), stillborn (S male) natural reproduction (N using bisulphite sequencing polymerase chain reaction BS ‐ PCR expression levels these were also...
The present study was undertaken to examine the effects of cytoplasmic volume on nucleus reprogramming and developmental competence buffalo handmade cloning (HMC) embryos. We found that both HMC embryos derived from ~150% cytoplasm or ~225% resulted in a higher blastocyst rate total cell number comparison with those ~75% (25.4 ± 2.0, 27.9 1.6% vs. 17.9 3.1%; 150 10, 169 12 85 6, P<0.05). Meanwhile, proportions nuclear envelope breakdown (NEBD) premature chromosome condensation (PCC) were...
The present study explored a suitable parthenogenetic activation (PA) procedure for rabbit oocytes and investigated the developmental potential of somatic cell nuclear transfer (SCNT) embryos using foetal fibroblasts (RFFs). electrical had optimal rate blastocyst (14.06%) when were activated by three direct current (DC) pulses (40 V/mm, 20 μs each) followed 6-dimethylaminopurine (6-DMAP) cycloheximide (CHX) treatment; ionomycin (ION) + 6-DMAP CHX (12.07%) was higher than that ION (8.6%) or...
Isolation and culture of spermatogonial stem cells (SSCs) are attractive for production genetic modified offspring. In the present study, buffalo stem-like were isolated, cultured expression pattern different germ cell marker genes determined. To recover spermatogonia, testes from age 3 to 7 months decapsulated, seminiferous tubules enzymatically dissociated. Two types cells, immature sertoli type A spermatogonia observed in this stage. Germ genes, OCT3/4 (Pou5f1), THY-1, c-kit, PGP9.5...
Neutral alpha-1,4-glucosidase (NAG) is a crucial biomarker for the function of epididymis and reported to be associated with semen quality. However, correlation between NAG Chinese quality has never been reported. This study aimed investigate level in seminal plasma men. A total 394 cases samples from normal, subfertile infertile men were enrolled this study. Male subfertility was caused by teratozoospermia, asthenospermia, severe oligozoospermia, asthenoteratozoospermia, oligoasthenospermia...
Wnt/β-Catenin signaling pathway plays an important role in maintaining self-renewal and pluripotency of human mouse embryonic stem cells (ESCs). Activation by glycogen synthase kinase-3 (GSK3) inhibitor, the Wnt agonist, could maintain ESCs presence serum. However, derivation buffalo remains unclear. In this study, we used GSK3 inhibitors (6-bromoindirubin-3′-oxime [BIO] CHIR99021) investigated effect activation on colony formation, proliferation, self-renewal, Chinese swamp (buffalo)...