A5101715088- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Renal and related cancers
- Epigenetics and DNA Methylation
- Retinal Imaging and Analysis
- Retinal Diseases and Treatments
- Sperm and Testicular Function
- Cancer-related molecular mechanisms research
- Bacteriophages and microbial interactions
- Glaucoma and retinal disorders
- Prenatal Screening and Diagnostics
- DNA and Nucleic Acid Chemistry
- Effects and risks of endocrine disrupting chemicals
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Retinal and Optic Conditions
- Metal complexes synthesis and properties
- Selenium in Biological Systems
- Genetic and phenotypic traits in livestock
- Tissue Engineering and Regenerative Medicine
- RNA Research and Splicing
- MRI in cancer diagnosis
- TGF-β signaling in diseases
Guangdong Academy of Medical Sciences
2023-2025
Southern Medical University
2023-2025
Guangdong Provincial People's Hospital
2023-2025
Shenzhen University
2023
Shenzhen Institutes of Advanced Technology
2021-2022
Guangxi University
2012-2021
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2011-2021
Chinese Academy of Sciences
2021
Indiana University School of Medicine
2000
Indiana University – Purdue University Indianapolis
2000
Background: The asymptomatic onset and extremely high mortality rate of aortic aneurysm (AA) highlight the urgency early detection timely intervention. alteration retinal vascular features (RVFs) can reflect systemic properties, be widely used as biomarker for cardiovascular disease risk prediction. Therefore, we aimed to investigate associations RVFs with AA its progression. Methods: In this prospective population-based cohort study, participants eligible fundus images without a history at...
Ectopically, expression of defined factors could reprogram mammalian somatic cells into induced pluripotent stem (iPSCs), which initiates a new strategy to obtain cell lines. Attempts have been made generate buffalo by culturing primary germ or inner mass, but the efficiency is extremely low. Here, we report successful method fetal fibroblasts (BFFs) [buffalo (biPSCs)] transduction (Oct4, Sox2, Klf4, and c-Myc) using retroviral vectors. The established biPSCs displayed typical morphological...
Efforts have been made to reduce the genomes of living cells, but phage genome reduction remains challenging. It is great interest investigate whether can make phages obtain new infectious properties. We developed a CRISPR/Cas9-based iterative (CiPGr) approach and applied this four distinct phages, thereby obtaining heterogeneous genome-reduced mutants. isolated sequenced 200 mutants with loss up 8-23% (3.3-35 kbp) original sequences. This allowed identification non-essential genes for...
Background: Identifying the fundus objective biomarkers for major depressive disorders (MDD) may help promote mental health.The aim of this study was to evaluate retinal neurovascular changes and further investigate their association with disease severity in MDD.Methods: This cross-sectional conducted hospital enrolled patients MDD healthy controls.The parameters all subjects, including vessel density (VD), thickness ganglion cell complex (GCC) nerve fiber layer (RNFL), optic head (ONH) eg...
Incomplete epigenetic reprogramming of donor cell nuclei is one the main contributors to low efficiency somatic nuclear transfer (SCNT). To improve success SCNT, DNA methylation levels must be reduced those found in totipotent embryonic cells. Recent studies have demonstrated that miR-148a can affect via DNMT1 modulation various cancers. Therefore, focus this study was examine influence on cells and SCNT embryo development. Thus, a stable line overexpressing established used produce embryos....
Abstract The possibility of producing transgenic cloned buffalos by nuclear transfer fetal fibroblasts expressing enhanced green fluorescent protein (EGFP) was explored in this study. When buffalo (BFFs) isolated from a male fetus were transfected with pEGFP-N1 ( EGFP is driven CMV and Neo SV-40) means electroporation, Lipofectamine-LTX X-tremeGENE, the transfection efficiency electroporation (35.5%) higher than (11.7%) X-tremeGENE (25.4%, P < 0.05). BFFs more embryos pEGFP-IRES-Neo are...
Acrylonitrile (ACN) is a monomer used in the synthesis of rubber, fibers and plastics. Previous studies demonstrated that ACN induces brain neoplasms (predominately astrocytomas) rats following chronic treatment. While mechanisms ACN-induced glial cell carcinogenicity have not been completely elucidated, investigations by our group others suggested role for induction oxidative stress resultant damage this process. In vitro transformation models are useful detecting studying chemical...
At present, many three-dimensional (3D) culture systems have been reported, improving the oocyte quality of in vitro maturation (IVM), yet mechanism still needs to be further explored. Here we examined effects a new self-made 3D glass scaffold on buffalo maturation; meanwhile, underlying was also detected. Compared two-dimensional (2D) dish culture, results revealed that can improve first polar body rate oocytes, subsequent cleavage and blastocysts parthenogenetic activation embryos (p <...
The present study was undertaken to examine the effect of Scriptaid treatment on histone acetylation, DNA methylation, expression genes related and development buffalo somatic cell nuclear transfer (SCNT) embryos. Treatment SCNT embryos with 500 nM for 24 h resulted in a significant increase blastocyst formation rate (28.2% vs. 13.6%, p<0.05). Meanwhile, also higher acetylation levels H3K18 lower methylation global at stage, which similar fertilized counterparts. CBP, p300, HAT1, Dnmt1,...
Abstract Somatic cell nuclear transfer (SCNT) holds vast potential in agriculture. However, its applications are still limited by low efficiency. Histone 3 lysine 9 trimethylation (H3K9me3) was identified as an epigenetic barrier for this. demethylase KDM4D could regulate the level of H3K9me3. effects on buffalo SCNT embryos unclear. Thus, we performed this study to explore and underlying mechanism embryos. The results revealed that compared with IVF embryos, expression significantly lower...
Buffalo are characteristic livestock of the Guangxi Zhuang Autonomous Region China, but their low reproductive capacity necessitates use somatic cell nuclear transfer (SCNT). We investigated effects RG108 on DNA methylation in buffalo adult fibroblasts, and subsequent SCNT embryo development. treatment (0, 5, 10, 20, 100 mM) had no effect morphology, viability, or karyotype (2n = 48), growth followed a typical "S" curve. Immunohistochemistry showed that relative gradually decreased as...
Fat deposition is one of the most important traits that are mediated by a set complex regulatory factors in meat animals. Several researches have revealed significant role long non-coding RNAs (lncRNAs) fat while precise mechanism still largely elusive. In this study, we investigated lncRNA profiles adipose and muscle tissues buffalo using Illumina HiSeq 3000 platform. total, 43,809 lncRNAs were finally identified based on computer algorithm. A comparison analysis 241 differentially...
The present study was undertaken to examine the effects of cytoplasmic volume on nucleus reprogramming and developmental competence buffalo handmade cloning (HMC) embryos. We found that both HMC embryos derived from ~150% cytoplasm or ~225% resulted in a higher blastocyst rate total cell number comparison with those ~75% (25.4 ± 2.0, 27.9 1.6% vs. 17.9 3.1%; 150 10, 169 12 85 6, P<0.05). Meanwhile, proportions nuclear envelope breakdown (NEBD) premature chromosome condensation (PCC) were...
The present study explored a suitable parthenogenetic activation (PA) procedure for rabbit oocytes and investigated the developmental potential of somatic cell nuclear transfer (SCNT) embryos using foetal fibroblasts (RFFs). electrical had optimal rate blastocyst (14.06%) when were activated by three direct current (DC) pulses (40 V/mm, 20 μs each) followed 6-dimethylaminopurine (6-DMAP) cycloheximide (CHX) treatment; ionomycin (ION) + 6-DMAP CHX (12.07%) was higher than that ION (8.6%) or...
The sperm protein IZUMO1 plays a central role in gamete fusion. In mouse sperm, is enriched at the acrosomal cap before acrosome reaction, and equatorial segment following this reaction; its relocation dependent on filamentous actin. How actin polymerization affects during interaction remains unknown. present study addressed these processes using latrunculin A (LatA), an inhibitor of polymerization. We report that 25 µM LatA blocked capacitated head, resulting marked decrease with relocated...
Contents Low efficiency of somatic cell nuclear transfer (SCNT) embryos is largely attributable to imperfect reprogramming the donor nucleus. The differences in epigenetic between female and male buffalo cloned remain unclear. We explored effects sex on development SCNT embryos. then compared expression DNA methylation (5‐methylcytosine‐5mC 5‐hydroxymethylcytosine‐5hmC) level relevant genes, histone (H3K9me2 H3K9me3) SCNT‐♀ SCNT‐♂ preimplantation with vitro fertilization (IVF) counterparts....
Signal transducer and activator 5 (STAT5) plays important roles in regulating mammary glandular cell proliferation milk-protein gene expression. However, the functions of STAT5a STAT5b genes lactation buffalo remain uninvestigated. In this study, full-length (2502 bp) (2515 coding sequences were isolated for first time. The highest expression was found glands brains glands. H&E staining indicated that are mainly expressed epithelial cells Next, we investigated STAT5 by knocking down...