A5100361744- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Sirtuins and Resveratrol in Medicine
- DNA Repair Mechanisms
- PARP inhibition in cancer therapy
- Autophagy in Disease and Therapy
- Calcium signaling and nucleotide metabolism
- Nerve injury and regeneration
- Neuroscience and Neural Engineering
- Biochemical effects in animals
- Intensive Care Unit Cognitive Disorders
- Metabolism and Genetic Disorders
- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- Telomeres, Telomerase, and Senescence
- Genetic Neurodegenerative Diseases
- Cytokine Signaling Pathways and Interactions
- Cancer-related molecular mechanisms research
- Immune Cell Function and Interaction
- Metabolomics and Mass Spectrometry Studies
- Cancer, Lipids, and Metabolism
- Advanced Nanomaterials in Catalysis
- RNA regulation and disease
Anhui Agricultural University
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2025
Jinan University
2011-2025
Chongqing University
2025
Chongqing Cancer Hospital
2025
Southwest Hospital
2024-2025
Peking Union Medical College Hospital
2013-2025
Albany Medical Center Hospital
2024
Anhui Medical University
2015-2024
Second Hospital of Anhui Medical University
2024
It's a SIRT Intense attention has focused on the SIRT1 deacetylase as possible target for anti-aging drugs. But unexpected complications in assays of activity have made it unclear whether compounds thought to be sirtuin-activating (STACs) are really direct regulators enzyme. Further exploration these effects by Hubbard et al. (p. 1216 ; see Perspective Yuan and Marmorstein ) revealed that interaction with certain substrates allows activation STACs identified critical amino acids required...
Cellular metabolic memory occurs in diabetic microvascular and macrovascular complications, but the underlying mechanisms remain unclear. Here, we investigate role of sirtuin 1 (SIRT1) metformin this phenomenon. In bovine retinal capillary endothelial cells (BRECs) retinas rats, inflammatory gene, nuclear factor-κB (NF-κB), proapoptotic Bax, induced by hyperglycemia, remained elevated after returning to normoglycemia. BRECs with small interfering RNA-mediated SIRT1 knockdown had increased...
<h3>Importance</h3> In adults undergoing hip fracture surgery, regional anesthesia may reduce postoperative delirium, but there is uncertainty about its effectiveness. <h3>Objective</h3> To investigate, in older surgical repair for fracture, the effects of on incidence delirium compared with general anesthesia. <h3>Design, Setting, and Participants</h3> A randomized, allocation-concealed, open-label, multicenter clinical trial 950 patients, aged 65 years older, or without preexisting...
The 5'-->3'-exonuclease domain of Escherichia coli DNA polymerase I is required for the completion lagging strand synthesis, and yet this not present in any eukaryotic polymerases. Recently, gene encoding functional evolutionary equivalent has been identified. It called FEN-1 mouse human cells RTH1 Saccharomyces cerevisiae. This 42-kDa enzyme Okazaki fragment processing. Here we report that physically interacts with proliferating cell nuclear antigen (PCNA), processivity factor polymerases...
NAD + can influence DNA repair by modulating protein interactions.
A new two-photon fluorescence probe for endogenous peroxynitrite (ONOO–) detection was designed and synthesized. The exhibits good selectivity sensitivity ONOO– in phosphate-buffered saline solution with a low limit (3.5 × 10–8 M). Furthermore, the displays performance detecting ONOO–, not only RAW 264.7 cells but also rat hippocampal tissue, high cross-section value (δ ≈ 100 GM) at deep depth of 120 μm.
The harsh local micro-environment following spinal cord injury (SCI) remains a great challenge for neural regeneration. Local reconstitution of favorable by biocompatible scaffolds with desirable functions has thus been an area concern. Herein, hybrid hydrogel was developed using Fmoc-grafted chitosan (FC) and Fmoc peptide (FI). Dynamic reversible π-π stacking interactions the fluorenyl rings enabled FC/FI to exhibit excellent injectable self-healing properties, as characterized visual...
Mitochondria are vital for energy production and redox homeostasis, yet knowledge of relevant mechanisms remains limited. Here, through a genome-wide CRISPR-Cas9 knockout screening, we have identified DMT1 as major regulator mitochondria membrane potential. Our findings demonstrate that deficiency increases the activity mitochondrial complex I reduces III. Enhanced leads to increased NAD+ production, which activates IDH2 by promoting its deacetylation via SIRT3. This results in higher levels...
Minocycline is neuroprotective in clinical and experimental stroke studies, due part to its ability inhibit poly (ADP-ribose) polymerase. Previous preclinical data have shown that interference with polymerase signaling leads sex-specific neuroprotection, reducing injury only males. In this study, we show minocycline ineffective at ischemic damage females after middle cerebral artery occlusion, likely effects on signaling. Clinical trials must consider possible sex differences the response...
Adaptor proteins (AP 1–5) are heterotetrameric complexes that facilitate specialized cargo sorting in vesicular-mediated trafficking. Mutations AP5Z1, encoding a subunit of the AP-5 complex, have been reported to cause hereditary spastic paraplegia (HSP), although their impact at cellular level has not assessed. Here we characterize three independent fibroblast lines derived from skin biopsies patients harbouring nonsense mutations AP5Z1 and presenting with accompanied by neuropathy,...
Electrical stimulation (ES) can be used to manipulate recovery after peripheral nerve injuries. Although biomaterial-based strategies have already been implemented gain momentum for ES and engineer permissive microenvironments neural regeneration, the development of biomaterials specific stimuli-responsive modulation cell properties remains a challenge. Herein, we homogeneously incorporate pristine carbon nanotubes into functional self-assembling peptide prepare hybrid hydrogel with good...
Background: Cyanotic congenital heart disease (CCHD) is a complex pathophysiological condition involving systemic chronic hypoxia (CH). Some patients with CCHD are unoperated for various reasons and remain chronically hypoxic throughout their lives, which heightens the risk of failure as they age. Hypoxia activates cellular metabolic adaptation to balance energy demands by accumulating hypoxia-inducible factor 1-α (HIF-1α). This study aims determine effect CH on cardiac metabolism function...
Background and Purpose— Emerging data suggest that the molecular cell death pathways triggered by ischemic insults differ in male female brain. Cell males is initiated poly(ADP-ribose) polymerase-1 (PARP-1) activation; however, manipulation of this pathway paradoxically increases damage females. In contrast, females are exquisitely sensitive to caspase-mediated death. The effect caspase inhibition PARP-1 knockout mice was evaluated determine if detrimental effects PARP deletion were...
The DNA repair capacity of human cells declines with age, in a process that is not clearly understood. Mutation the nuclear envelope protein barrier-to-autointegration factor 1 (Banf1) has previously been shown to cause progeroid disorder, Néstor-Guillermo progeria syndrome (NGPS). underlying links between Banf1, and ageing are unknown. Here, we report Banf1 controls damage response oxidative stress via regulation poly [ADP-ribose] polymerase (PARP1). Specifically, lesions promote direct...