We report a ratiometric two-photon probe (SSH-Mito) for mitochondrial thiols. This shows marked blue-to-yellow emission color change in response to RSH, significant cross section, good thiol selectivity, low cytotoxicity, and insensitivity pH over the biologically relevant range, allowing direct visualization of RSH levels live cells as well living tissues at 90-190 μm depth without interference from other species through use microscopy.

We report a two-photon fluorescent probe (ASS) that can be excited by 780 nm femtosecond pulses and detect thiols in live cells living tissues at 90−180 μm depth without interference from other biologically relevant species microscopy.

A naphthalimide-based highly selective colorimetric and ratiometric fluorescent probe for the fluoride ion displayed both one- two-photon changes. Upon reaction with F− (TBA+ Na+ salts) anion in CH3CN as well aqueous buffer solution, 1 shows dramatic color changes from colorless to jade-green remarkable fluorescence enhancements signals. These properties are mechanistically ascribed a fluoride-triggered Si−O bond cleavage that resulted green 4-amino-1,8-naphthalimide.

We report a two-photon probe (SZn-Mito) for mitochondrial zinc ions ([Zn2+]m). This shows 7-fold enhancement of two-photon-excited fluorescence in response to Zn2+ with dissociation constant (Kd(TP)) 3.1 ± 0.1 nM and pH insensitivity the biologically relevant range, allowing detection [Zn2+]m rat hippocampal slice at depth 100−200 μm without interference from other metal through use microscopy.

We report a two-photon probe (FS1) which shows 21-fold excited fluorescence enhancement in response to H(2)S and can selectively detect rat hippocampal slice at depth of 90-190 μm by using microscopy.

We report a two-photon fluorescent probe (PN1) that can be excited by 750 nm femto-second pulses, shows high photostability and negligible toxicity, visualize H2O2 distribution in live cells tissue microscopy.

We report a two-photon fluorescent probe (SHP-Mito) which can ratiometrically detect mitochondrial H2O2 in live cells and intact tissues at >100 μm depth through the use of microscopy.

Hydrogen sulfide (H2S) is a signaling gasotransmitter, which plays various roles in modulating the functions of different systems. In this study, "turn-on" fluorescent probe for H2S was developed. The probe, whose design based on familiar excited state intramolecular proton transfer (ESIPT) fluorophore bearing aggregation-induced emission (AIE) characteristics, found to exhibit an 80-fold fluorescence enhancement along with large Stokes shift upon addition H2S. Furthermore, successfully...

A new two-photon fluorescence probe for endogenous peroxynitrite (ONOO–) detection was designed and synthesized. The exhibits good selectivity sensitivity ONOO– in phosphate-buffered saline solution with a low limit (3.5 × 10–8 M). Furthermore, the displays performance detecting ONOO–, not only RAW 264.7 cells but also rat hippocampal tissue, high cross-section value (δ ≈ 100 GM) at deep depth of 120 μm.

A series of pyrene derivatives having 4-(N,N-dimethylamino)phenylethynyl groups as the substituent (1-5) have been synthesized and their two-photon absorption properties were investigated. Comparison cross section (delta(max)) with related compounds reveals that is efficient a pi-center anthracene in materials. Moreover, increased number substituents reaching at maximum value 1150 GM for tetra-substituted derivative (5). Furthermore, action (Phi delta(max)) 5 comparable to most This result...

Two photons are better than one: A two-photon probe (ANa1, see picture) shows strong fluorescence enhancement in response to Na+ and a dissociation constant (Kdi) of (26±2) mM the cell. It can be easily loaded into cells selectively detect intracellular free live living tissues at depth 100–200 μm for long period time. Detailed facts importance specialist readers published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted...

pH values go live! A ratiometric two-photon (TP) probe (NP1, see scheme) that has a significant TP action cross-section, high photostability, negligible toxicity, and can estimate in live cells human tissues by microscopy is described. NP1 detect the difference between from gastroesophageal junction (GEJ) lower esophageal sphincter of patients with without esophagitis (see image). Detailed facts importance to specialist readers are published as "Supporting Information". Such documents...

Copper ions play a crucial role in living systems as cofactors of numerous metalloenzymes. To quantitatively estimate the Cu2+ concentration human tissue, we have developed two-photon (TP) probe with an internal reference (ACCu2) that shows significant TP action cross-section and high selectivity for can colon tissues by dual-color microscopy (TPM) imaging minimum interference from other competing metal or pH cytotoxicity photostability problems. The concentrations normal colon, polyp,...

We report a highly sensitive two-photon probe (SZn2-Mito) which shows 70-fold excited fluorescence enhancement in response to Zn2+ and can selectively detect mitochondrial rat hippocampal slice at depth of 100–200 μm by using microscopy.

In this study, we developed a two-photon fluorescent probe for detection of peroxynitrite (ONOO–) near the N-methyl-d-aspartate (NMDA) receptor. This naphthalimide-based contains boronic acid reactive group and an ifenprodil-like tail, which serves as NMDA receptor targeting unit. The displays high sensitivity selectivity, along with fast response time in aqueous solution. More importantly, can be employed fluorescence microscopy to detect endogenous ONOO– receptors neuronal cells well...

Excited-state intramolecular proton transfer (ESIPT)-based fluorophores with two-photon excitation fluorescence (TPEF) are rare. Our aim this research was to develop ESIPT-based exhibiting TPEF. Herein, we used 4-hydroxyisoindoline-1,3-dione as a scaffold fluorescent probe BHID-Bpin, for the detection of peroxynitrite (ONOO-). BHID-Bpin exhibits excellent selectivity, sensitivity, and fast response towards ONOO- in PBS buffer solution (10 mM, pH = 7.40). Additionally, displays high...

We report a two-photon fluorescent probe (AHg1) that can be excited by 780 nm femto-second pulses, shows high photostability and negligible toxicity, visualize the site of Hg2+ accumulation, but also estimate trace amounts mercury ions in fresh fish organs microscopy.

We developed a fluorescent pH probe (<bold>1</bold>) capable of two-photon excitation and far-visible-emission based on FRET, composed naphthalimide–piperazine–rhodamine.

Currently, the role of transient receptor potential vanilloid type 4 (TRPV4), a nonselective cation channel in pathology spinal cord injury (SCI), is not recognized. Herein, we report expression and contribution TRPV4 scarring endothelial secondary damage after SCI. increased during inflammatory phase female rats SCI was expressed primarily by cells at endothelial-microglial junctions. Two-photon microscopy intracellular-free Ca 2+ levels revealed biphasic increase similar time points...

An effective approach to the design of photosensitizers was proposed and applied in two-photon excited photodynamic therapy cancer.

Azo containing dyes as a two-photon selective and type I based photosensitizers (PSs) were developed that exhibit excellent photodynamic therapy properties under mild condition.

N-Methyl-d-aspartate (NMDA) is an excitotoxic amino acid used to identify a specific subset of glutamate receptors. The activity NMDA receptors closely related the redox level biological system. Glutathione (GSH) as antioxidant plays key role with regard modulation environment. In this work we designed and developed GSH-specific fluorescent probe capability targeting receptors, which was composed two-photon naphthalimide fluorophore, GSH-reactive group sulfonamide, ifenprodil for receptor....

We report a two-photon fluorescent probe (ACu1) that can be excited by 750 nm femto-second pulses, shows high photostability and negligible toxicity, visualize Cu(+) distribution in live cells tissues microscopy.

It takes two: Two-photon tracers (AG1, AG2) have been developed that can be excited by 780 nm fs laser pulses, easily loaded into cancer cells and tissues, show high photostability negligible toxicity, visualize glucose uptake in living tissues two-photon microscopy (TPM). Moreover, AG2 screen anticancer agents at 75–150 mm depth TPM. Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset....