A5050463748- Pain Mechanisms and Treatments
- Ion Channels and Receptors
- Ion channel regulation and function
- Neuropeptides and Animal Physiology
- Neuroscience and Neuropharmacology Research
- Dermatology and Skin Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Biochemical effects in animals
- Immune Response and Inflammation
- Fatty Acid Research and Health
- Urticaria and Related Conditions
- Mesenchymal stem cell research
- Eicosanoids and Hypertension Pharmacology
- Herbal Medicine Research Studies
- Erythrocyte Function and Pathophysiology
- Nanoplatforms for cancer theranostics
- interferon and immune responses
- Pharmacological Effects of Natural Compounds
- Neuroscience of respiration and sleep
- Nerve injury and regeneration
- Olfactory and Sensory Function Studies
- Seaweed-derived Bioactive Compounds
- Lung Cancer Diagnosis and Treatment
- Menstrual Health and Disorders
- Exercise and Physiological Responses
Chonnam National University
2015-2025
Chonnam National University Hwasun Hospital
2015-2025
Gachon University
2015-2024
University of Cincinnati
2022-2024
University of Cincinnati Medical Center
2024
Gachon University Gil Medical Center
2024
Hanyang University
2022
Chonnam National University Hospital
2017-2021
Korea Institute of Fusion Energy
2017
Duke Medical Center
2013-2016
Self-resolving inflammatory exudates and lipid mediator metabolomics recently uncovered a new family of potent anti-inflammatory proresolving mediators biosynthesized by macrophages (MΦs), denoted maresins. Here we determined that maresin 1 (MaR1) produced human MΦs from endogenous docosahexaenoic acid (DHA) matched synthetic 7R,14S-dihydroxydocosa-4Z,8E,10E,12Z,16Z,19Z-hexaenoic acid. The MaR1 alcohol groups Z/E geometry conjugated double bonds were using isomers prepared total organic...
Accumulating evidence suggests that spinal cord astrocytes play an important role in neuropathic pain sensitization by releasing astrocytic mediators (e.g. cytokines, chemokines and growth factors). However, it remains unclear how control the release of sustain late-phase pain. Astrocytic connexin-43 (now known as GJ1) has been implicated gap junction hemichannel communication cytosolic contents through glial syncytia to extracellular space, respectively. Connexin-43 also plays essential...
Inflammatory pain such as arthritic is typically treated with opioids and cyclo-oxygenase-2 inhibitors well known side effects. Transient receptor potential subtype vanilloid 1 (TRPV1) TRP ankyryn (TRPA1) contribute importantly to the genesis of inflammatory via both peripheral mechanisms (peripheral sensitization) spinal cord (central sensitization). Although these channels have been intensively studied, little about their endogenous inhibitors. Recent studies demonstrated that lipid...
Mechanisms of inflammatory pain are not fully understood. We investigated the role TRPV1 (transient receptor potential subtype V1) and TNF-α, two critical mediators for pain, in regulating spinal cord synaptic transmission. found mice lacking Trpv1 frequency but amplitude spontaneous EPSCs (sEPSCs) lamina II neurons slices is reduced. Further, C-fiber-induced long-term potentiation (LTP) vivo abolished knock-out mice. TNF-α also increases sEPSC outer (lamina IIo) neurons, this increase...
Neuropathic pain remains a pressing clinical problem. Here, we demonstrate that local, intrathecal (i.t.) injection of bone marrow stromal cells (BMSCs) following lumbar puncture alleviates early- and late-phase neuropathic symptoms, such as allodynia hyperalgesia, for several weeks in murine chronic constriction injury (CCI) spared nerve models. Moreover, i.t. BMSCs reduced CCI-induced spontaneous axonal dorsal root ganglion (DRG) neurons inhibited CCI-evoked neuroinflammation DRGs spinal...
Epoxyeicosatrienoic acids (EETs) are cytochrome P450-epoxygenase-derived metabolites of arachidonic acid that act as endogenous signaling molecules in multiple biological systems. Here we have investigated the specific contribution 5,6-EET to transient receptor potential (TRP) channel activation nociceptor neurons and its consequence for nociceptive processing. We found that, during capsaicin-induced nociception, levels increased dorsal root ganglia (DRGs) spinal cord, is released from...
Prevalence of neuropathic pain is high after major surgery. However, effective treatment for preventing lacking. Here we report that perisurgical neuroprotectin D1/protectin D1 (NPD1/PD1), derived from docosahexaenoic acid, prevents nerve injury-induced mechanical allodynia and ongoing in mice. Intrathecal post-treatment NPD1/PD1 also effectively reduces established produces no apparent signs analgesic tolerance. Mechanistically, blocks long-term potentiation, glial reaction, inflammatory...
Significance Chemotherapy-induced peripheral neuropathic pain (CIPNP) is a severe side effect that affects up to 80% of patients during cancer treatment. Pharmacological treatment options are poor, as CIPNP differs mechanistically from other chronic states. Here, we describe mechanism by which pronociceptive oxidized lipid mediators generated the cytochrome P 450 -epoxygenase CYP2J2 in sensory neurons CIPNP. Blocking synthesis these lipids with telmisartan, identified potent inhibitor large...
Microglia, resident immune cells in the central nervous system, play a role neuroinflammation and development of neuropathic pain. We found that stimulator interferon genes (STING) is predominantly expressed spinal microglia upregulated after peripheral nerve injury. However, mechanical allodynia, as marker pain following injury, did not require microglial STING expression. In contrast, activation by specific agonists (ADU-S100, 35 nmol) significantly alleviated male mice, but female mice....
Temperature signaling can be initiated by members of transient receptor potential family (thermo-TRP) channels. Hot and cold substances applied to teeth usually elicit pain sensation. This study investigated the expression thermo-TRP channels in dental primary afferent neurons rat identified retrograde labeling with a fluorescent dye maxillary molars. Single cell reverse transcription-PCR immunohistochemistry revealed TRPV1, TRPM8, TRPA1 subsets such neurons. Capsaicin (a TRPV1 agonist),...
Eugenol is widely used in dentistry as a local analgesic agent, because of its ability to allay tooth pain. Interestingly, eugenol shares several pharmacological actions with anesthetics which include inhibition voltage-gated sodium channel (VGSC) and activation transient receptor potential vanilloid subtype 1 (TRPV1). In the present study, we investigated effects on pain behaviors orofacial area, an attempt elucidate mechanism characterized inhibitory VGSCs trigeminal ganglion (TG) neurons....
It is well established that activation of NMDARs plays an essential role in spinal cord synaptic plasticity (i.e., central sensitization) and pain hypersensitivity after tissue injury. Despite prominent expression DRG primary sensory neurons, the unique peripheral regulating intrinsic neuronal excitability sensitivity not understood, part due to lack selective molecular tools. To address this problem, we used Advillin-Cre driver delete NR1 subunit selectively neurons. In conditional...
Piezo channels are mechanosensitive ion channels. Piezo1 is primarily expressed in nonsensory tissues, whereas Piezo2 predominantly found sensory including dorsal root ganglion (DRG) neurons. However, a recent study demonstrated the intracellular calcium response to Yoda1, selective agonist, trigeminal (TG) Herein, we investigate expression of mRNA and protein mouse human DRG neurons activation via influx by Yoda1. Yoda1 induces inward currents mainly small- (<25 μm) medium-sized (25–35...
ANXA11 dysfunction induces alteration of intracellular Ca 2+ homeostasis and stress granule disassembly in ALS.
Psoriasis is a chronic inflammatory disease caused by complex interplay between the immune and nervous systems with recurrent scaly skin plaques, thickened stratum corneum, infiltration activation of cells, itch. Despite an increasing availability therapies, they often have adverse effects, high costs, dissociated effects on inflammation Activation sensory neurons innervating TRPV1 (transient receptor potential vanilloid 1) are emerging as critical components in pathogenesis psoriasis, but...
Satellite glial cells (SGCs) are among the most abundant non-neuronal in dorsal root ganglia (DRGs) and closely envelop sensory neurons that detect painful stimuli. However, little is still known about their homeostatic activities contribution to pain. Using single-cell RNA sequencing (scRNA-seq), we were able obtain a unique transcriptional profile for SGCs. We found enriched expression of tissue inhibitor metalloproteinase 3 (TIMP3) other metalloproteinases Small interfering neutralizing...
Gamma-aminobutyric acid (GABA) neuronal system-related transcription factors (TFs) play a critical role in GABA production, and modulates diabetic neuropathic pain (DNP). The present study investigated the therapeutic effects of intrathecal delivery two TFs achaete-scute homolog 1 (Ascl1) LIM homeobox protein 6 (Lhx6) mouse model DNP elucidated their underlying mechanisms. GABA-related specific TFs, including Ascl1, Lhx6, distal-less 1, 5, Nkx2.1 gene, Nkx2.2 were under normal conditions....
Transient receptor potential vanilloid subtype 1 (TRPV1) and metabotropic glutamate 5 (mGluR5) located on peripheral sensory terminals have been shown to play critical roles in the transduction modulation of pain sensation. To date, however, very little is known regarding significance functional expression mGluR5 TRPV1 central neurons dorsal horn spinal cord. Here we show that presynaptic coupled a membrane-delimited manner, thereby contributing nociceptive synaptic transmission substantia...
Abstract Mechanisms of acute pain transition to chronic are not fully understood. Here we demonstrate an active role β-arrestin 2 (Arrb2) in regulating spinal cord NMDA receptor (NMDAR) function and the duration pain. Intrathecal injection mu-opioid agonist [D-Ala , NMe-Phe 4 Gly-ol 5 ]-enkephalin produces paradoxical behavioural responses: early-phase analgesia late-phase mechanical allodynia which requires NMDAR; both phases prolonged Arrb2 knockout (KO) mice. Spinal administration induces...