A5034107200- Ion Channels and Receptors
- Pain Mechanisms and Treatments
- Ion channel regulation and function
- Salivary Gland Disorders and Functions
- Pancreatic function and diabetes
- Neuropeptides and Animal Physiology
- Erythrocyte Function and Pathophysiology
- Glycosylation and Glycoproteins Research
- Ion Transport and Channel Regulation
- Amyotrophic Lateral Sclerosis Research
- S100 Proteins and Annexins
- Adenosine and Purinergic Signaling
- Chemical and Physical Studies
- Neuroscience and Neuropharmacology Research
- Endoplasmic Reticulum Stress and Disease
- Respiratory and Cough-Related Research
- Biochemical Analysis and Sensing Techniques
- Asthma and respiratory diseases
- Adipose Tissue and Metabolism
- Lipid Membrane Structure and Behavior
- Neuroscience of respiration and sleep
- Plant Molecular Biology Research
- Allergic Rhinitis and Sensitization
- Venomous Animal Envenomation and Studies
- Cancer-related gene regulation
Gachon University
2019-2025
University of Cincinnati
2024
University of Cincinnati Medical Center
2024
Gachon University Gil Medical Center
2024
Seoul National University Dental Hospital
2008-2014
Seoul National University
2009-2014
Gyeongsang National University
2009
University of Illinois Urbana-Champaign
2009
Guangxi University
2009
Purdue University West Lafayette
2009
Abstract The contribution of SOS1 (for Salt Overly Sensitive 1), encoding a sodium/proton antiporter, to plant salinity tolerance was analyzed in wild-type and RNA interference (RNAi) lines the halophytic Arabidopsis (Arabidopsis thaliana)-relative Thellungiella salsuginea. Under all conditions, mRNA abundance higher than Arabidopsis. Ectopic expression homolog ThSOS1 suppressed salt-sensitive phenotype Saccharomyces cerevisiae strain lacking sodium ion (Na+) efflux transporters increased...
Piezo channels are mechanosensitive ion channels. Piezo1 is primarily expressed in nonsensory tissues, whereas Piezo2 predominantly found sensory including dorsal root ganglion (DRG) neurons. However, a recent study demonstrated the intracellular calcium response to Yoda1, selective agonist, trigeminal (TG) Herein, we investigate expression of mRNA and protein mouse human DRG neurons activation via influx by Yoda1. Yoda1 induces inward currents mainly small- (<25 μm) medium-sized (25–35...
ANXA11 dysfunction induces alteration of intracellular Ca 2+ homeostasis and stress granule disassembly in ALS.
Gamma-aminobutyric acid (GABA) neuronal system-related transcription factors (TFs) play a critical role in GABA production, and modulates diabetic neuropathic pain (DNP). The present study investigated the therapeutic effects of intrathecal delivery two TFs achaete-scute homolog 1 (Ascl1) LIM homeobox protein 6 (Lhx6) mouse model DNP elucidated their underlying mechanisms. GABA-related specific TFs, including Ascl1, Lhx6, distal-less 1, 5, Nkx2.1 gene, Nkx2.2 were under normal conditions....
Mature adults often exhibit higher pain thresholds than younger individuals. However, this phenomenon is poorly understood, especially with regards to peripheral nervous system signaling. We investigated the involvement of amyloid beta (Aβ) in regulating heat sensitivity within dorsal root ganglion (DRG) during adult maturation. employed various vivo and vitro techniques investigate modulatory effect Aβ1-42. Heat alteration was examined spared nerve injury (SNI) models young mature...
Gamma-aminobutyric acid (GABA)ergic neurons play a key role in pain modulation within the dorsal root ganglion (DRG), making them critical targets for therapeutic studies. This study utilized F11 cells as an vitro model to examine GABAergic function under high-glucose conditions mimicking diabetic neuropathy. Differentiated exhibited increased sensory neuronal marker expression and functional action potentials. Overexpression of transcription factor Achaete-scute homolog 1 (Ascl1) via...
Objectives. Recently, autoantibodies directed against muscarinic type 3 receptor (M3R) have been reported in patients with primary SS. However, the precise epitope(s) of M3R that interacts SS remains unclear. The aim this study was to identify functional epitope which immunoglobulin G (IgG). Methods. Purified IgGs were obtained from sera seven (six and one secondary SS) two normal persons. We examined whether IgG inhibits function identified using six synthetic peptides covering all...
Abstract Hormonal regulation during food ingestion and its association with pain prompted the investigation of impact glucagon-like peptide-1 (GLP-1) on transient receptor potential vanilloid 1 (TRPV1). Both endogenous synthetic GLP-1, as well a GLP-1R antagonist, exendin 9–39, reduced heat sensitivity in naïve mice. GLP-1-derived peptides (liraglutide, exendin-4, 9–39) effectively inhibited capsaicin (CAP)-induced currents calcium responses cultured sensory neurons TRPV1-expressing cell...
Salivary gland epithelial cells (SGEC) release several cytokines that play important roles in the inflammatory process. In this study, we examined whether capsaicin can modulate cytokine SGEC. After were stimulated with polyinosinic-polycytidylic acid [poly(I:C)] or lipopolysaccharide (LPS), mRNA transcript and protein levels detected by reverse-transcriptase-polymerase chain-reaction (RT-PCR), real-time PCR, enzyme-linked immunosorbent assay (ELISA). These findings demonstrated increases...
One of the common side effects antihistamine medicines is xerostomia (dry mouth). The current consensus that antihistamine-induced comes from an antimuscarinic effect. Although effect antihistamines on salivary secretion both obvious and significant, cellular mechanism whereby this happens still unclear because lack knowledge histamine signaling in human glands. Here, we have studied receptors submandibular acinar cells. In primary cultured gland a HSG cell line, increased intracellular...
Local anesthetics (LAs) can completely block nociception by inhibiting voltage-gated sodium channels (VGSCs), and thus, blocking action potentials (APs) within sensory neurons. As one of the several LAs, eugenol is used for dental pain treatment. It reportedly features multiple functions in regulating diverse ion channels. This study aimed to investigate long-lasting analgesic effect alone, as well that combination a noxious-heat-sensitive transient receptor potential vanilloid 1 (TRPV1)...
Oral Diseases (2012) 18 , 132–139 Objectives: Autoantibodies from the sera of Sjögren’s syndrome patients (SS IgG) have been suggested to inhibit muscarinic receptor function. However, acute nature such an inhibitory effect remains controversial. In this study, we investigated effects SS IgG on function in human submandibular gland (HSG) cells. Methods: The autoantibodies were studied using microspectrofluorimetry, whole‐cell patch clamp, immunofluorescence confocal microscopy, and a...
Hormonal regulation during food ingestion and its association with pain prompted the investigation of impact glucagon-like peptide-1 (GLP-1) on transient receptor potential vanilloid 1 (TRPV1). Both endogenous synthetic GLP-1 an antagonist GLP-1, exendin 9-39, reduced heat sensitivity in naïve mice. GLP-1-derived peptides (liraglutide, exendin-4, 9-39) effectively inhibited capsaicin (CAP)-induced currents calcium responses cultured sensory neurons TRPV1-expressing cell lines. Notably, 9-39...
Painful diabetic neuropathy commonly affects the peripheral nervous system in individuals with diabetes. However, pathological processes and mechanisms underlying neuropathic pain remain unclear. We aimed to identify overall profiles screen for genes potentially involved using transcriptome analysis of dorsal root ganglion mice treated streptozotocin (STZ). Using RNA sequencing, we identified differentially expressed (DEGs) between streptozotocin-treated controls, focusing on altered...
High concentrations of ATP induce membrane blebbing. However, the underlying mechanism involved in epithelial cells remains unclear. In this study, we investigated role P2×7 receptor (P2×7R) blebbing using Par C5 cells. We stimulated with 5 mM for 1 ~ 2 hrs and found characteristics blebbing, a hallmark apoptotic cell death. addition, 500 μM Bz-ATP, specific P2×7R agonist, induced 300 Ox-ATP, antagonist, inhibited ATP-induced suggesting that is mediated by P2×7R. was ROCK I activation MLC...
Autoantibodies specific for alpha-fodrin fragments are found in the tissues of persons afflicted with Sjögren’s syndrome (SS). However, mechanism degradation remains elusive. The following experiments utilized Par C5 cells to examine role P2X7 receptor (P2X7R) apoptosis, particularly cleavage and release alpha-fodrin, an apparent SS autoantigen. Five mM ATP stimulation induced apoptotic cell death a sustained Ca 2+ influx, which was mimicked HEK transfected P2X7R. also mediated by caspase-3...
Transient receptor potential vanilloid subtype 1 (TRPV1) was originally found in sensory neurons. Recently, it has been reported that TRPV1 is expressed salivary gland epithelial cells (SGEC). However, the physiological role of secretion remains to be elucidated. We mouse and human submandibular glands (SMG) HSG cells, originated from ducts at both mRNA protein levels. capsaicin (CAP), agonist, had little effect on intracellular free calcium concentration ([Ca(2+)]i) these although carbachol...
Mechanosensitive ion channels, particularly Piezo are widely expressed in various tissues. However, their role immune cells remains underexplored. Therefore, this study aimed to investigate the functional of Piezo1 human eosinophil cell line AML14.3D10. We detected