A5004832741- Ion channel regulation and function
- Salivary Gland Disorders and Functions
- Ion Transport and Channel Regulation
- Pain Mechanisms and Treatments
- Biochemical Analysis and Sensing Techniques
- Ion Channels and Receptors
- Tissue Engineering and Regenerative Medicine
- Epigenetics and DNA Methylation
- Neonatal Respiratory Health Research
- Neuroscience and Neuropharmacology Research
- Cardiac electrophysiology and arrhythmias
- Neuropeptides and Animal Physiology
- Pancreatic function and diabetes
- Amino Acid Enzymes and Metabolism
- Barrier Structure and Function Studies
- Diabetes and associated disorders
- Immune Response and Inflammation
- Lipid Membrane Structure and Behavior
- Receptor Mechanisms and Signaling
- Adenosine and Purinergic Signaling
- Hippo pathway signaling and YAP/TAZ
- Advanced Nanomaterials in Catalysis
- Nicotinic Acetylcholine Receptors Study
- Connexins and lens biology
- Pharmacological Effects of Natural Compounds
Seoul National University Dental Hospital
2009-2022
Seoul National University
2012-2021
Korea University
2004
University of Manchester
2001
The activation of spinal cord glial cells has been implicated in the development neuropathic pain upon peripheral nerve injury. molecular mechanisms underlying cell activation, however, have not clearly elucidated. In this study, we found that damaged sensory neurons induce expression tumor necrosis factor-alpha, interleukin-1beta, interleukin-6, and inducible nitric-oxide synthase genes cells, which is pain. Studies using primary isolated from toll-like receptor 2 knock-out mice indicate...
Glial activation is known to contribute pain hypersensitivity following spinal sensory nerve injury. In this study, we investigated mechanisms by which glial cell in medullary dorsal horn (MDH) would tactile inferior alveolar and mental transection (IAMNT). Activation of microglia astrocytes was monitored at 2 h, 1, 3, 7, 14, 28, 60 days using immunohistochemical analysis with OX-42 GFAP antibodies, respectively. Tactile significantly increased 1 day, lasted for 28 after IAMNT. Microglial...
Abstract Nanomaterials with antioxidant properties are promising for treating reactive oxygen species (ROS)‐related diseases. However, maintaining efficacy at low doses to minimize toxicity is a critical clinical applications. Tuning the surface strain of metallic nanoparticles can enhance catalytic reactivity, which has rarely been demonstrated in metal oxide nanomaterials. Here, it shown that inducing strains CeO 2 /Mn 3 O 4 nanocrystals produces highly antioxidants protect tissue‐resident...
In this study, we investigated purinergic receptor P2X7 and NACHT, LRR PYD domains-containing protein 3 (NLRP3) inflammasome expressions, their role in head neck cancer. We found upregulation of all NLRP3 components biopsied squamous cell carcinoma tissues. Similarly, the expression P2X7, apoptosis-associated speck-like containing CARD, pro-form caspase 1 A253 cells derived from epidermoid were highly upregulated comparison to normal Human Salivary Gland line. Active caspase-1 its final...
Abstract Viral infection is one of the leading causes brain encephalitis and meningitis. Recently, it was reported that Toll‐like receptor‐3 (TLR3) induces a double‐stranded RNA (dsRNA)‐mediated inflammatory signal in cells innate immune system, studies suggested dsRNA may induce inflammation central nervous system (CNS) by activating CNS‐resident glial cells. To explore further connection between CNS, we have studied effects stimulation astrocytes. Our results show injection...
Temperature signaling can be initiated by members of transient receptor potential family (thermo-TRP) channels. Hot and cold substances applied to teeth usually elicit pain sensation. This study investigated the expression thermo-TRP channels in dental primary afferent neurons rat identified retrograde labeling with a fluorescent dye maxillary molars. Single cell reverse transcription-PCR immunohistochemistry revealed TRPV1, TRPM8, TRPA1 subsets such neurons. Capsaicin (a TRPV1 agonist),...
The pathophysiology of exocrine dysfunction observed in Sjögren's syndrome (SS) is not fully understood. purpose this study was to investigate whether autoantibodies against human AQP5 are present the sera SS patients. Frozen sections mouse submandibular salivary glands, CHO cells over-expressing a AQP5–GFP fusion protein or GFP, and MDCK were used indirect immunofluorescence assay detect anti-AQP5 from patients with primary SS. lysates HEK-293 GFP for immunoprecipitation. Serum IgG but...
Recently, there has been growing interest in replacing severely damaged salivary glands with artificial gland functional units created vitro by tissue engineering approaches. Although various materials such as poly(lactic-co-glycolic acid), polylactic acid, poly(glycolic and polyethylene glycol hydrogels have used scaffolds for engineering, none of them is effective enough to closely recapitulate the branched structural complexity heterogeneous cell population native glands. Instead...
One of the common side effects antihistamine medicines is xerostomia (dry mouth). The current consensus that antihistamine-induced comes from an antimuscarinic effect. Although effect antihistamines on salivary secretion both obvious and significant, cellular mechanism whereby this happens still unclear because lack knowledge histamine signaling in human glands. Here, we have studied receptors submandibular acinar cells. In primary cultured gland a HSG cell line, increased intracellular...
Peri-implantitis is a major cause of dental implant failure. Bacterial biofilm contamination on the induces surrounding bone resorption and soft tissue inflammation, leading to severe deterioration oral health. However, conventional removal procedures, such as mechanical decontamination antiseptic application, are not effective enough induce reosseointegration decontaminated surfaces. This due (1) incomplete from inaccessible areas (2) physicochemical alteration surfaces caused by...
The effect of intracellular acidification and subsequent pH recovery in sensory neurons has not been well characterized. We have studied the mechanisms underlying Ca(2+)-induced (pH(i)) rat trigeminal ganglion report their effects on neuronal excitability. Glutamate (500 μM) capsaicin (1 increased Ca(2+) concentration ([Ca(2+)](i)) with a following decrease pH(i). [Ca(2+)](i) to prestimulus level was inhibited by LaCl(3) mM) o-vanadate (10 mM), plasma membrane Ca(2+)/ATPase (PMCA) inhibitor....
MMP-9-dependent proteolysis of histone H3 N-terminal tail (H3NT) is an important mechanism for activation gene expression during osteoclast differentiation. Like other enzymes targeting their substrates within chromatin structure, MMP-9 enzymatic activity toward H3NT tightly controlled by modifications such as H3K18 acetylation (H3K18ac) and H3K27 monomethylation (H3K27me1). Growing evidence indicates that DNA methylation another epigenetic controlling osteoclastogenesis, but whether also...
Inflammation in the peripheral nervous system (PNS) is one of characteristics virus-induced neuropathy. In this inflammatory response, Schwann cells are actively involved. Previously, toll-like receptor 3 (TLR3) was reported as a for double-stranded RNA (dsRNA) that induces antiviral and responses innate immune system. study, we investigated expression putative role TLR3 cells. constitutively expressed Stimulation with polyinosinic-polycytidylic acid, synthetic dsRNA analogue, induced...