A5063216279- Cell Adhesion Molecules Research
- Platelet Disorders and Treatments
- Atrial Fibrillation Management and Outcomes
- Atherosclerosis and Cardiovascular Diseases
- Heparin-Induced Thrombocytopenia and Thrombosis
- Chemokine receptors and signaling
- Venous Thromboembolism Diagnosis and Management
- Acute Ischemic Stroke Management
- Advanced Biosensing Techniques and Applications
- Drug Transport and Resistance Mechanisms
- Pain Mechanisms and Treatments
- Microfluidic and Capillary Electrophoresis Applications
- Antiplatelet Therapy and Cardiovascular Diseases
- Innovative Microfluidic and Catalytic Techniques Innovation
- Peptidase Inhibition and Analysis
- Inflammatory mediators and NSAID effects
- Pharmacogenetics and Drug Metabolism
- Synthesis of β-Lactam Compounds
- Analytical Methods in Pharmaceuticals
- Protein purification and stability
- Protease and Inhibitor Mechanisms
- Free Radicals and Antioxidants
- Cardiac Arrhythmias and Treatments
- ECG Monitoring and Analysis
- Histone Deacetylase Inhibitors Research
CS Diagnostics
2023
Roche Pharma AG (Germany)
2023
Fraunhofer Institute for Translational Medicine and Pharmacology
2018
Goethe University Frankfurt
2007-2017
University Hospital Frankfurt
2014-2017
Institute of Molecular Biology
2016
Fraunhofer Institute for Molecular Biology and Applied Ecology
2016
Significance Chemotherapy-induced peripheral neuropathic pain (CIPNP) is a severe side effect that affects up to 80% of patients during cancer treatment. Pharmacological treatment options are poor, as CIPNP differs mechanistically from other chronic states. Here, we describe mechanism by which pronociceptive oxidized lipid mediators generated the cytochrome P 450 -epoxygenase CYP2J2 in sensory neurons CIPNP. Blocking synthesis these lipids with telmisartan, identified potent inhibitor large...
Platelets are well known for their role in hemostasis but also increasingly recognized supporting innate immune responses. Here, we studied the of platelets development peripheral inflammation and found that colocalize with macrophages inflamed tissue outside blood vessels different animal models cutaneous inflammation. Collagen-treatment isolated from paws during zymosan-induced induced thromboxane synthesis through platelet-expressed collagen receptor glycoprotein VI. Deletion VI or its...
A universal coagulation test that reliably detects prolonged time in patients, irrespective of the anticoagulant administered, has not been available to date. An easily miniaturised, novel μ-fluidic employing surface acoustic waves (SAW) is presented here. SAW was employed instantly mix and recalcify 6 μl citrated whole blood image correlation analysis used quantify clot formation kinetics. The detection clinically relevant dosing with old anticoagulants (unfractionated heparin, argatroban)...
ABSTRACT Inflammatory pain is based on stimulation and sensitization of peripheral endings sensory neurons (nociceptors) by pronociceptive mediators. These mediators can be released resident cells, as well invading immune cells. Although neutrophils are known to release various mediators, which stimulate or sensitize nociceptors, the extent their contribution nociceptive responses unclear. Here, we studied zymosan-induced inflammatory pain, characterized an early recruitment high numbers...
This article intends to close the gap between abundance of regular articles focusing on adhesive mechanisms cells in a flow field and purely technical reports confined description newly developed algorithms, not yet ready be used by users without programming skills. A simple robust method is presented for analysing raw videomicroscopic data flow-based adhesion assays using freely available public domain software ImageJ. We describe detail image processing routines rapidly reliably evaluate...
Endothelial chemokine CXC motif ligand 16 (CXCL16) expression is associated with atherosclerosis, while platelets, particularly those attaching to atherosclerotic plaque, contribute all stages of disease. This investigation was designed examine the role CXCL16 in capturing platelets from flowing blood. expressed human plaques, and lesion severity carotid endarterectomy specimens positively correlated levels. plaques co-localised deposited endothelium. Immobilised promoted CXCR6-dependent...
Calibration of Prothrombin Time (PT) in terms the International Normalized Ratio (INR) has been outlined 'Guidelines for thromboplastins and plasmas used to control oral anticoagulant therapy' (World Health Organization, 2013). Standard ISO 17511:2020 presents requirements manufacturers In-vitro Diagnostic Medical Devices (IVD MD) documenting calibration hierarchy a measured quantity human samples using specified IVD MD. The objective this article is define an unequivocal, metrologically...
The influence of the histone deacetylase (HDAC)-inhibitor, valproic acid (VPA), on bladder cancer cell adhesion in vitro was investigated this paper. TCCSUP and RT-112 cells were treated with VPA (0.5 or 1 mM) twice thrice weekly for 14 days. Controls remained untreated. Tumour interaction immobilized collagen evaluated by a flow-based assay using shear force 2 4 dyne/cm . effects integrin receptors α3, α5, β1, β3 β4 assessed flow cytometry to determine surface expression western blotting...
Reliable detection of anticoagulation status in patients treated with non-vitamin K antagonist oral anticoagulants (NOACs) is challenging but importance especially the emergency setting. This study evaluated potential a whole-blood clotting time assay based on Surface Acoustic Waves (SAW-CT) stroke-patients. The SAW-technology was used for quick and homogenous recalcification whole blood inducing surface-activated reaction quantified visualised by real-time fluorescence microscopy automatic...
Exchange of the lipophilc part ortho-substituted cinnamic acid lead structures with different small molecule fluorophoric moieties via a dimethylene spacer resulted in hEP3R ligands affinities nanomolar concentration range. Synthesized compounds emit fluorescence blue, green, and red range light have been tested concerning their potential as pharmacological tool. hEP3Rs were visualized by confocal laser scanning microscopy on HT-29 cells, murine kidney tissues, human brain tissues...
Clinicians demand for methods to monitor effects of direct anticoagulants in the emergency setting. We recently described a coagulation assay based on surface acoustic waves (SAW) technology, which quantifies anticoagulant by image processing. Here we describe first step miniaturizing this laboratory method and provide portable prototype that contains optical illumination automatic on-board processing.A device about size shoebox was realized SAW-chip, signal generator, LED illumination, as...
The RGD cyclic pentapetide, cilengitide, is a selective inhibitor of αvβ3 and αvβ5 integrins was developed for antiangiogenic therapy. Since cilengitide interacts with platelet αIIbβ3 platelets express αv integrins, the effect on pro-coagulative response adhesion interest. Flow-based assays were performed to evaluate rolling von Willebrand factor (vWf), fibrinogen human umbilical vein endothelial cells (HUVECs). Flow cytometry used detect activation (PAC1) secretion (CD62P) by light...