A5014070406- Sleep and related disorders
- Sleep and Wakefulness Research
- Asthma and respiratory diseases
- Obstructive Sleep Apnea Research
- Respiratory and Cough-Related Research
- DNA Repair Mechanisms
- Circadian rhythm and melatonin
- Neuroscience of respiration and sleep
- Receptor Mechanisms and Signaling
- IL-33, ST2, and ILC Pathways
- Eosinophilic Esophagitis
- Inhalation and Respiratory Drug Delivery
- Neurotransmitter Receptor Influence on Behavior
- Cardiovascular Function and Risk Factors
- Electronic Health Records Systems
- CRISPR and Genetic Engineering
- Healthcare Technology and Patient Monitoring
- Restless Legs Syndrome Research
- Biomedical and Engineering Education
- Cardiovascular Health and Disease Prevention
- Synthesis and Biological Evaluation
- Microtubule and mitosis dynamics
- Synthetic Organic Chemistry Methods
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- T-cell and B-cell Immunology
University of Nebraska Medical Center
2020-2024
Hunter Medical Research Institute
2022-2024
John Hunter Hospital
2020-2024
Hunter New England Local Health District
2023
Princess Alexandra Hospital
2021
The University of Queensland
2021
Royal Hallamshire Hospital
2020
National Health Service
2020
St. Jude Children's Research Hospital
2017
Eastern Virginia Medical School
2017
This guideline establishes clinical practice recommendations for the use of behavioral and psychological treatments chronic insomnia disorder in adults. The American Academy Sleep Medicine (AASM) commissioned a task force experts sleep medicine psychology to develop assign strengths based on systematic review literature an assessment evidence using Grading Recommendations Assessment, Development Evaluation (GRADE) methodology. evaluated summary relevant quality evidence, balance clinically...
Severe asthma is a high-burden disease. Real-world data on mepolizumab in patients with severe eosinophilic needed to assess whether the from randomised controlled trials are applicable broader population. The Australian Mepolizumab Registry (AMR) was established an aim use, effectiveness and safety of for Australia. Patients (n=309) (median age 60 years, 58% female) commenced mepolizumab. They had poor symptom control Asthma Control Questionnaire (ACQ)-5 score 3.4), frequent exacerbations...
The C family G-protein-coupled receptors contain members that sense amino acid and extracellular cations, of which calcium-sensing receptor (CASR) is the prototypic receptor. Some cells, such as osteoblasts in bone, retain responsiveness to calcium CASR-deficient mice, consistent with existence another We examined properties GPRC6A, a newly identified member this family. Alignment GPRC6A CASR revealed conservation both calcimimetic binding sites. In addition, calcium, magnesium, strontium,...
The 5'-->3'-exonuclease domain of Escherichia coli DNA polymerase I is required for the completion lagging strand synthesis, and yet this not present in any eukaryotic polymerases. Recently, gene encoding functional evolutionary equivalent has been identified. It called FEN-1 mouse human cells RTH1 Saccharomyces cerevisiae. This 42-kDa enzyme Okazaki fragment processing. Here we report that physically interacts with proliferating cell nuclear antigen (PCNA), processivity factor polymerases...
Structure-specific nucleases catalyze critical reactions in DNA replication, recombination, and repair. Recently, a structure-specific endonuclease, FEN-1, has been purified shown to cleave flap structures. Here, we describe the cloning of murine FEN-1 gene. The nucleotide sequence is highly homologous Saccharomyces cerevisiae genes YKL510 RAD2. We show that truncated RAD2 protein are also endonucleases. substrate specificity implicates branched structures as important intermediates excision...
GPRC6A is a widely expressed orphan G-protein coupled receptor that senses extracellular amino acids, osteocalcin and divalent cations in vitro. The physiological functions of are unknown.In this study, we created characterized the phenotype GPRC6A(-/-) mice. We observed complex metabolic abnormalities mice involving multiple organ systems express GPRC6A, including bone, kidney, testes, liver. exhibited hepatic steatosis, hyperglycemia, glucose intolerance, insulin resistance. In addition,...
<i>Background:</i> The prevalence and nature of sleep disorders in primary care has not been widely studied. As part a survey conducted 5 family practice offices North Carolina, we screened adult patients for syndromes sought to ascertain which demographic status health were associated with these disorders. <i>Methods:</i> We approached 2963 consecutive adults who presented office visits the study practices. 4-page questionnaire, was available English Spanish, included items on insomnia,...
Chronic airway diseases including asthma and COPD are prevalent high-burden conditions with the majority of patients successfully managed in primary care setting. However, for some more complex disease such as difficult-to-treat or severe asthma, COPD, tertiary is required. This review provides an overview successful multidisciplinary respiratory service that operates Newcastle, New South Wales, Australia, which has been integrated into outpatient clinics almost three decades. The...
Wildfires are increasing and cause health effects. The immediate ongoing impacts of prolonged wildfire smoke exposure in severe asthma unknown. This longitudinal study examined the experiences (bushfire) adults with during 2019/2020 Australian bushfire period. Participants from Eastern/Southern Australia who had previously enrolled an registry completed a questionnaire survey regarding symptoms, attacks, quality life mitigation bushfires months following exposure. Daily individualized to...
Abstract Background Eosinophils are key therapeutic targets in severe asthma that suppressed by IL5 (mepolizumab) and receptor (benralizumab) blockade. The effect of pathway biologics on recently described homeostatic (hEOs) inflammatory (iEOs) eosinophil subsets is unknown. We aimed to determine the relative impact mepolizumab benralizumab treatment subset phenotype, explore clinical associations with characteristics response. Methods performed a cross‐sectional observational study...
In eukaryotic cells, a 5′-flap DNA endonuclease and double-stranded 5′-exonuclease activity reside within 42-kDa enzyme called FEN-1 (flap endonuclease-1 5(five)′-exonuclease). This endo/exonuclease has been shown to be highly homologous human XP-G, Saccharomyces cerevisiae RAD2, S. YKL510. Like FEN-1, these related structure-specific nucleases recognize cleave branched structure flap its derivative, pseudo Y-structure. To dissect the important structural components of structure, we have...
The NF-kappaB pathway plays a critical role in regulating cellular processes such as immune responses, stress apoptosis, proliferation and differentiation, whereas dysfunction of this has been associated with numerous cancer disorders. We have applied our Random Activation Gene Expression technology to an reporter cell line facilitate the discovery positive regulators activation. A small protein expression library, corresponding approximately 0.1x genome coverage, was generated screened for...
The Valsalva maneuver was evaluated by echocardiography in three groups: A) 10 normal volunteers, B) patients with no history of heart failure and ejection fractions, C) depressed fractions. Groups A B had a significant fall left ventricular internal dimensions calculated stroke volume end strain which returned rapidly to baseline recovery without overshoot. Arterial pressure showed signoidal pattern overshoot early all group patients. In C did not diminish during strain; arterial pressures...
Abstract Background and Objective Type 2 (T2) innate lymphoid cells (ILC2s) contribute to airway inflammation disease in asthma. We hypothesize that ILC2s isolated from people with severe allergic eosinophilic asthma would exhibit an enhanced T2 inflammatory activity be altered following treatment mepolizumab omalizumab. compare peripheral blood (PB) ILC2's proliferative capacity, IL‐5 IL‐13 secretion phenotype between healthy without (HC), non‐asthma (NAA), mild (MA) (SA) subjects. then...
Murine severe combined immune deficiency (scid) is marked by a 5,000-fold reduction in coding joint formation V(D)J recombination of antigen receptors. Others have demonstrated sensitivity to double-strand breaks generated ionizing radiation and bleomycin. We were interested establishing the extent defect intramolecular intermolecular DNA end joining lymphoid nonlymphoid cells from scid mice. conducted series studies probing ability these resolve free ends linear molecules having various...