GPRC6A is a widely expressed orphan G-protein coupled receptor that senses extracellular amino acids, osteocalcin and divalent cations in vitro. The physiological functions of are unknown.In this study, we created characterized the phenotype GPRC6A(-/-) mice. We observed complex metabolic abnormalities mice involving multiple organ systems express GPRC6A, including bone, kidney, testes, liver. exhibited hepatic steatosis, hyperglycemia, glucose intolerance, insulin resistance. In addition,...

The objective was to compare biomarkers of articular cartilage metabolism in synovial fluid from Hartley guinea pig knees, with and without anterior cruciate ligament transection (ACLT), establish whether detectable differences biomarker levels exist between primary secondary osteoarthritis (OA). Synovial lavages knees were obtained 3-month (control group) 12-month (primary OA animals. Another group animals (posttraumatic underwent unilateral ACLT at 3 months, samples 9 months postsurgery....

Runx2 controls the commitment of mesenchymal cells to osteoblastic lineage. Distinct promoters, designated P1 and P2, give rise functionally similar Runx2-II Runx2-I isoforms. We postulate that this dual promoter gene structure permits temporal spatial adjustments in amount isoforms necessary for optimal bone development. To evaluate dose-dependent effect on development, we intercrossed selective Runx2-II(+/-) with nonselective Runx2-II(+/-)/Runx2-I(+/-) mice create compound mutant mice:...

The goals of this study were to investigate the role Notch1/PDGFRβ/ROCK1 signaling pathway in pathogenesis pulmonary fibrosis and explore possibility treating by targeting Notch1. Lung tissues from patients with examined for expression using RT-qPCR, western blotting, immunostaining. Cultured mouse lung pericytes transfected Notch1-overexpressed vectors or shRNA PDGFRβ/ROCK1 examine cell behaviors, including proliferation, cycle arrest, differentiation toward myofibroblasts. Finally, a model...

Postoperative pain is caused by surgical injury and trauma; stressful to patients; includes a series of physiologic, psychological, behavioral reactions. Effective postoperative analgesia helps improve pain, perioperative safety, hospital discharge rates. This study aimed observe the influence intravenous sufentanil patient-controlled combined with music therapy versus alone on hemodynamics in patients lung cancer.This was randomized parallel performed 60 American Society Anesthesiologists...

Polycystin-1 (PC1) may play an important role in skeletogenesis through regulation of the bone-specific transcription factor Runx2-II. In current study we found that PC1 co-localizes with calcium channel polycystin-2 (PC2) primary cilia MC3T3-E1 osteoblasts. To establish Runx2-II mediating effects on bone, crossed heterozygous Pkd1m1Bei and mice to create double (Pkd1+/m1Bei/Runx2-II+/-) deficient both Pkd1+/m1Bei/Runx2-II+/- exhibited additive reductions expression was associated impaired...

Targeted expression of gene technique is an important therapeutic strategy for lung cancer. MicroRNA-7 has been well documented as a promising tumor suppressor but never test in specific gene-promoter-targeted cancer therapy. Here, we first evaluated the efficacy miR-7 operated by promoter TTF-1, lineage-specific oncogene cancer, vitro using eukaryotic vector TTF-1-promoter-operated (termed p-T-miR-7). Interestingly, nude mice model, growth and metastasis human cells vivo were significantly...

BACKGROUND:Rab-like 3 (Rabl3) is a member of the Rab subfamily small GTPases which are involved in controlling proliferation and vesicular trafficking. Recent studies suggest that proteins might play critical role regulating cancer cell survival, but underlying mechanisms remain largely unknown. MATERIAL AND METHODS:We performed bioinformatics analysis to examine correlation between expression level Rabl3 survival non-small lung (NSCLC) patients three independent cohorts containing 484...

In this study, we first generated and characterized a polyclonal antibody against unique domain of matrlin-2 then used specific to assess the expression pattern matrilin-2 by immunohistochemistry. We found that marilin-2 is widely distributed in connective tissues many mouse including heart, colon, penis, esophagus, lung, kidney, tracheal cartilage, developmental bone, adult bone. The level was remarkably increased osteoarthritis articular compared normal healthy tissues. Furthermore,...

// Yichun Wang 1 , Ying Liang 2 Junming Luo Jing Nie Huiming Yin 3 Qiong Chen Dong Jixiang Zhu Jiamei Xia and Wei Shuai Critical Care Medicine Department, Hunan Cancer Hospital, Changsha, China Department of Food Science Engineering, Central South University Forestry Technology, Respiratory The First Affiliated Hospital Medicine, Correspondence to: Yin, email: 1976841746@qq.com Keywords: XIST/miR-139, pulmonary fibrosis (PF), fibroblast, β-catenin, extracellular matrix (ECM) Received: April...

The abscopal effect has previously been described in various tumors and is associated with radiation therapy hyperthermia, possible underlying mechanisms explaining each observed case. In the present study, we aimed to investigate antitumor effects of magnet-mediated hyperthermia on Walker-256 carcinosarcomas rats at two different temperature ranges (42-46°C 50-55°C). We also identify whether a higher therapeutic magnetic-mediated improves effects, where localised irradiation tumor causes...

Type 1 diabetes (T1D) is an autoimmune disease characterized by the destruction of beta cells immune cells. The interactions among within islets may be closely linked to pathogenesis T1D. In this study, we used single-cell RNA sequencing (scRNA-Seq) analyze cellular heterogeneity a T1D mouse model. We established model induced streptozotocin and identified cell subpopulations using scRNA-Seq technology. Our results revealed 11 major types in pancreatic mice, with observed alpha subgroups,...

Synchronous gastric tumors that consist of both gastrointestinal stromal tumor (GIST) and adenocarcinoma are rare. We studied the clinicopathological molecular characteristics six cases containing GIST. By means immunohistochemical analysis, all GIST cells expressed CD117, CD34 Dog1 in synchronous adenocarcinomas with GIST, alone. Sequencing analysis demonstrated exon 11 c-kit mutations were present two four five GISTs. One GISTs was an uncommon mutation CTT > CCA at amino acid 576, other a...

Septic shock is one of the most significant health concerns across world, involving hypo-perfusion and defects in tissue energy.The current study investigates role NLR family CARD domain containing protein 4 (NLRC4) septic shock-induced inflammatory reactions, lung injuries, dendritic cell (DC) apoptosis.Septic mice models were established by modified cecal ligation puncture injected with retroviral vector expressing siRNA-NLRC4.DCs then isolated transfected siRNA-NLRC4.The degree injury,...

Abstract The von Willebrand Factor A (vWF A) domain is one of the most widely distributed structural modules in cell-matrix adhesive molecules such as intergrins and extracellular matrix proteins. Mutations vWF matrilin-3 cause multiple epiphyseal dysplasia (MED), however pathological mechanism remains to be determined. Previously we showed that matrilin-1 mediates formation a filamentous network through metal-ion dependent adhesion sites domain. Here show two new functions...

Pulmonary hypertension (PH) is defined as the mean pulmonary artery pressure (mPAP) ≥25 mmHg under sea level in resting state. ROCK1 and ROCK2 can be combined to cause damage of vascular endothelial function. To explore differences subjects with or normal plateau area, further understand mechanism Rho/rho-kinase pathway activation for promoting hypertension, we collected 64 patients 87 healthy controls. All were hospitalized Cardiology Respiration Department Qinghai Provincial Peoples'...

Abstract Background Codon 72 (Arg/Pro), the most frequently studied single nucleotide polymorphism (SNP) of p53 to date, is associated with ability gene induce cell apoptosis. The PI3K/Akt pathway plays an essential role in transcriptional activation function p53, and important factor radiotherapy resistance. present study was designed evaluate prediction response based on codon SNP pAkt expression biopsy specimens locoregional nasopharyngeal carcinoma (NPC) before treatment. Materials...

Chinese Hui medicine is a unique traditional system formed by the integration of Islamic Arabia and China medicine. It also cream ancient Eastern Western based on its concepts medical philosophy, such as theory Zhenyi Vitality seven elements. only national developed inheriting Arab philosophy integrating theory. likes to use application method characterized aromatic (also known ship medicine). has high treatment effect in orthopedics traumatology, brain, gynecology, ophthalmology,...

The aim of the present study was to examine cerebral vasoconstriction in patients with chronic high altitude disease [cerebrovascular reactivity (CVR)], and evaluate differences alterations brain vascular contractile mountain sickness (CMS) healthy controls. Alterations endothelin (ET) its receptor, as well endothelial nitric oxide synthase (eNOS) levels plasma were examined determine reservation capacities CMS patients. Transcranial Doppler ultrasound carbon dioxide analysis methods used...