A5000734385- Viral Infections and Immunology Research
- RNA regulation and disease
- interferon and immune responses
- RNA and protein synthesis mechanisms
- Cytomegalovirus and herpesvirus research
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- RNA Research and Splicing
- RNA modifications and cancer
- Endoplasmic Reticulum Stress and Disease
- Viral gastroenteritis research and epidemiology
- Cell death mechanisms and regulation
- MicroRNA in disease regulation
- Cardiovascular Effects of Exercise
- NF-κB Signaling Pathways
- Viral Infectious Diseases and Gene Expression in Insects
- Herpesvirus Infections and Treatments
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- Cardiac Structural Anomalies and Repair
- CRISPR and Genetic Engineering
- Virology and Viral Diseases
- Plant Virus Research Studies
- Cancer-related molecular mechanisms research
- Signaling Pathways in Disease
St. Paul's Hospital
2010-2024
University of British Columbia
2015-2024
St. Paul's Hospital
2011-2024
Heihe University
2021-2023
Harbin Veterinary Research Institute
2022
Chinese Academy of Agricultural Sciences
2022
Ministry of Public Security of the People's Republic of China
2020
Institute of Forensic Science
2020
Northeast Agricultural University
2020
East China Normal University
2015-2016
ABSTRACT Coxsackievirus B3 (CVB3) is the most common human pathogen for viral myocarditis. We have previously shown that signaling protein p21 ras GTPase-activating (RasGAP) cleaved and mitogen-activated kinases (MAPKs) ERK1/2 are activated in late phase of CVB3 infection. However, role intracellular pathways CVB3-mediated myocarditis relative advantages such to host or virus remain largely unclear. In this study we extended our prior studies by examining interaction between replication HeLa...
MicroRNAs (miRNAs) are a class of short non-coding RNAs with posttranscriptional regulatory functions. To date, more than 600 human miRNAs have been experimentally identified, and estimated to regulate one third cellular messenger RNAs. Accumulating evidence has linked the dysregulated expression patterns variety diseases, such as cancer, neurodegenerative cardiovascular diseases viral infections. MiRNAs provide its particular layer network for gene regulation, thus possessing great...
Coxsackievirus B3 (CVB3), an enterovirus in the family Picornaviridae, induces cytopathic changes cell culture systems and directly injures multiple susceptible organs tissues vivo, including myocardium, early after infection. Biochemical analysis of death pathway CVB3-infected HeLa cells demonstrated that 32-kDa proform caspase 3 is cleaved subsequent to degenerative morphological seen infected cells. Caspase activation assays confirm proteolytically active. The substrates poly(ADP-ribose)...
Coxsackievirus (CV)B3 is the primary cause of viral myocarditis. We previously observed CXC chemokine ligand 10 (CXCL10) upregulation in myocardium early infection. However, impact CXCL10 CVB3-induced myocarditis unknown. Using isolated mouse cardiomyocytes we demonstrated for first time that can express on interferon-gamma stimulation. To explore role myocarditis, both transgenic and knockout mice were used. Following CVB3 challenges, titer hearts inversely correlated with levels at phase...
ABSTRACT Cardiomyocyte apoptosis is a hallmark of coxsackievirus B3 (CVB3)-induced myocarditis. We used cardiomyocytes and HeLa cells to explore the cellular response CVB3 infection, with focus on pathways leading apoptosis. infection triggered endoplasmic reticulum (ER) stress differentially regulated three arms unfolded protein (UPR) initiated by proximal ER sensors ATF6a (activating transcription factor 6a), IRE1-XBP1 (X box binding 1), PERK (PKR-like kinase). Upon glucose-regulated 78...
Abstract —Host genetic responses that characterize enteroviral myocarditis have not yet been determined. The injurious and inflammatory process in heart muscle may reflect host of benefit to the virus ultimately result congestive failure dilated cardiomyopathy. On other hand, within myocardium secure against acute or protracted damage. To investigate nature modified gene expression comparison with normal tissue, mRNA species were assessed using cDNA microarray technology at days 3, 9, 30...
Coxsackievirus B3 (CVB3) is the most common causal agent of viral myocarditis, but existing drug therapies are limited value. Application small interfering RNA (siRNA) in knockdown gene expression an emerging technology antiviral therapy. To investigate whether interference (RNAi) can protect against CVB3 infection, we evaluated effects RNAi on replication HeLa cells and murine cardiomyocytes by using five CVB3-specific siRNAs targeting distinct regions genome. The effective one siRNA-4,...
Abstract Inflammatory cytokines play a key role in the myocardial injury produced by viral myocarditis. Although interleukin-6 (IL-6) reportedly possesses antiviral properties, its effect myocarditis is unclear. To investigate of IL-6 induced encephalomyocarditis virus (EMCV) mice, we evaluated (1) survival rate following administration, (2) titer heart, (3) replication heart situ hybridization, (4) histopathological changes using immunohistochemical staining, (5) neutralizing antibody...
Background Myocarditis is the major heart disease in infants and young adults. It very commonly caused by coxsackievirus B3 (CVB3) infection; however, no specific treatment or vaccine available at present. RNA interference (RNAi)-based anti-viral therapy has shown potential to inhibit viral replication, but this strategy faces two challenges; mutational escape from drug suppression targeted delivery of reagents cell populations. Methodology/Principal Findings In study, we designed artificial...
Intercalated disks (ICDs) are substantial connections maintaining cardiac structures and mediating signal communications among cardiomyocytes. Deficiency in ICD components such as desmosomes, fascia adherens gap junctions leads to heart dysfunction. Coxsackievirus B3 (CVB3) infection induces failure but its pathogenic effect on ICDs is unclear. Here we show that CVB3-induced miR-21 expression affects structure, i.e., upregulated targets YOD1, a deubiquitinating enzyme, enhance the K48-linked...
ABSTRACT Coxsackievirus group B3 (CVB3) replication is influenced by host cell cycle status. However, the effect of CVB3 infection on regulation and mechanisms involved are not precisely defined. In this study, we examined progression when was initiated in late G 1 phase cycle. Analysis cellular DNA synthesis infected cells thymidine incorporation assays showed a significant reduction [ 3 H]thymidine uptake compared to that sham-infected cells. To further clarify effects cycle, regulatory...
ABSTRACT Coxsackievirus B3 (CVB3) is a primary cause of viral myocarditis, yet no effective therapeutic against CVB3 available. Nucleic acid-based interventional strategies various viruses, including CVB3, have shown promise experimentally, but limited stability and inefficient delivery in vivo remain as obstacles to their potential therapeutics. We employed phosphorodiamidate morpholino oligomers (PMO) conjugated cell-penetrating arginine-rich peptide, P007 (to form PPMO), address these...
Gamma interferon-inducible protein 10 (IP10) is a member of the CXC family chemokines. By differential mRNA display, we have demonstrated upregulation IP10 in coxsackievirus B3 (CVB3)-infected mouse hearts. Functional characterization gene IP10-transfected Tet-On HeLa cells has found that induced cell apoptosis and inhibited viral replication. In IP10-induced apoptotic pathway, overexpression upregulated p53 resulted altered expression p53-responsive genes such as p21Cip1, p27kip1, NF-κB,...
Many virus infections and stresses can induce endoplasmic reticulum (ER) stress response, a host self-defense mechanism against viral invasion stress. During this event, cellular gene expression is actively regulated often encounters switching of the translation initiation from cap-dependent to internal ribosome-entry sites (IRES)-dependent. This largely dependent on mRNA structure 5' untranslated region (5' UTR) particular stimuli. Picornaviruses some other viruses contain IRESs within...