A5001422485- Plant-Microbe Interactions and Immunity
- Agricultural Economics and Practices
- Plant Parasitism and Resistance
- Plant Pathogens and Fungal Diseases
- Plant Stress Responses and Tolerance
- Smart Agriculture and AI
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Plant Genetic and Mutation Studies
- Photosynthetic Processes and Mechanisms
- Agricultural Science and Fertilization
- Plant Virus Research Studies
- Agricultural Systems and Practices
- Livestock Management and Performance Improvement
- Agricultural pest management studies
- Plant tissue culture and regeneration
- Plant pathogens and resistance mechanisms
- Plant Gene Expression Analysis
- Essential Oils and Antimicrobial Activity
- Fungal Biology and Applications
- Agriculture, Land Use, Rural Development
- Sirtuins and Resveratrol in Medicine
- Transgenic Plants and Applications
- Chemical Synthesis and Analysis
- Phytochemicals and Medicinal Plants
Chaudhary Devi Lal University
2020-2023
East Tennessee State University
2013-2022
Jawaharlal Nehru Technological University, Kakinada
2020
Andhra University
2020
Indian Institute of Science Bangalore
2018
Navsari Agricultural University
2017
All India Institute of Medical Sciences Bhopal
2016
All India Institute of Medical Sciences Raipur
2016
All India Institute of Medical Sciences
2016
Korea University of Science and Technology
2015
In plants, the mobile signal for systemic acquired resistance (SAR), an organism-wide state of enhanced defense to subsequent infections, has been elusive. By stimulating immune responses in mosaic tobacco plants created by grafting different genetic backgrounds, we showed that methyl salicylate (MeSA) esterase activity salicylic acid-binding protein 2 (SABP2), which converts MeSA into acid (SA), is required SAR perception tissue, tissue does not receive primary (initial) infection....
Any interaction of plants with phytopathogens involves the generation various chemical molecules that are critical for activation their defense machinery. One chemicals, salicylic acid (SA) induces systemic acquired resistance (SAR) in plants. The SAR provides a broad-spectrum against wide range related or unrelated pathogens. There has been considerable progress biochemical and molecular understanding In addition, several chemicals including SA its analogs known to provide direct indirect...
Salicylic acid (SA) is a critical signal for the activation of plant defense responses against pathogen infections. We recently identified SA-binding protein 2 (SABP2) from tobacco as that displays high affinity SA and plays crucial role in systemic acquired resistance to pathogens. Here we report crystal structures SABP2, alone complex with at up 2.1-Å resolution. The confirm SABP2 member α/β hydrolase superfamily enzymes, Ser-81, His-238, Asp-210 catalytic triad. bound active site...
Salicylic acid (SA) is a critical hormone for signaling innate immunity in plants. Here we present the purification and characterization of SA-binding protein 2 (SABP2), tobacco that low abundance specifically binds SA with high affinity. Sequence analysis predicted SABP2 lipase belonging to alpha/beta fold hydrolase super family. Confirming this prediction, recombinant exhibited activity against several synthetic substrates. Moreover, was stimulated by binding may generate lipid-derived...
Salicylic acid-binding protein 2 (SABP2) is essential for the establishment of systemic acquired resistance (SAR) in tobacco; SABP2's methyl salicylate (MeSA) esterase activity required healthy tissues infected plants to release active defense phytohormone SA from MeSA, which serves as a long-distance signal SAR. In current study, we characterize new gene family Arabidopsis thaliana encoding 18 potentially alpha/beta fold hydrolases that share 32-57% identity with SABP2. Of 14 recombinant...
In tobacco, two mitogen-activated protein (MAP) kinases, designated salicylic acid (SA)-induced kinase (SIPK) and wounding-induced (WIPK) are activated in a disease resistance-specific manner following pathogen infection or elicitor treatment. To investigate whether nitric oxide (NO), SA, ethylene, jasmonic (JA) involved this phenomenon, the ability of these defense signals to activate kinases was assessed. Both NO SA SIPK; however, they did not WIPK. Additional analyses with transgenic NahG...
The salicylic acid-induced protein kinase (SIPK) of tobacco, which is a mitogen-activated (MAPK), activated by various biotic and abiotic treatments. Overexpression SIPK has been shown to trigger cell death. In this study, targeted yeast two-hybrid approach identified the tobacco transcription factor WRKY1 as potential substrate. phosphorylated WRKY1, resulted in enhanced DNA-binding activity its cognate binding site, W box sequence from chitinase gene CHN50. SIPK-mediated enhancement was...
Tobacco SABP2, a 29kDa protein catalyzes the conversion of methyl salicylic acid (MeSA) into (SA) to induce SAR. Pretreatment plants with acibenzolar-S-methyl (ASM), functional analog induces systemic acquired resistance (SAR). Data presented in this paper suggest that SABP2 ASM acibenzolar Transgenic SABP2-silenced tobacco when treated ASM, fail express PR-1 proteins and do not robust SAR expression. When acibenzolar, full is induced plants. These results show required for ASM-mediated...
Purpose: The aim of the present study was to investigate wound healing activity selected Indian medicinal plant Quercus infectoria. Method: Ethanol extract shade-dried leaves infectoria studied for its effect on in rats, using incision, excision and dead-space models, at two different dose levels 400 800 mg/kg. Result: showed a definite, positive healing, with significant increase antioxidant enzymes, superoxide dismutase catalase, granuloma tissue. Conclusion: efficacy this may be due...
Tomatoes are considered one of the most valuable vegetables around world due to their usage and minimal harvesting period. However, effective still remains a major issue because tomatoes easily susceptible weather conditions other types attacks. Thus, numerous research studies have been introduced based on deep learning models for efficient classification tomato leaf disease. single architecture does not provide best results limited computational ability complexity. this used Transductive...
Summary RNA interference (RNAi) is widely used to specifically silence the expression of any gene study its function and identify validate therapeutic targets. Despite popularity this technology, recent studies have shown that RNAi may also non‐targeted genes. Here we demonstrate utility a quick, efficient robust approach directly specificity as an alternative indirect validation through profiling. Our involves reversing (complementing) RNAi‐induced phenotype by introducing synthetic version...
Santalum album Linn. [Family: Santalaceae] is commonly known as White sandalwood (English), Safed Chandan (Hindi) and Srigandha (Sanskrit). It found wildly cultivated in southern states of India. Traditionally, this plant used headache, fever inflammation. The wood oil diuretic, stimulant disinfective. Sandalwood contains a volatile 2.5–6%. main constituents are santalol, isovaleric aldehyde, santanone, santalone tannic acid. Based upon its traditional use chemical constituents, the was...
Systemic acquired resistance (SAR) is a state of heightened defense which induced throughout plant by an initial infection; it provides long-lasting, broad-spectrum to subsequent pathogen challenge. Recently we identified phloem-mobile signal for SAR has been elusive almost 30 years. It methyl salicylate (MeSA), inactive derivative the hormone, salicylic acid (SA). This discovery resulted from extensive characterization SA-binding protein 2 (SABP2), whose high affinity SA and extremely low...
A 69-kDa protein with topoisomerase I activity has been homogeneously purified from the chloroplasts of pea leaves. The properties are detected in crude lysate using technique transferring 32P radioactivity 32P-labeled DNA to protein. enzyme relaxes both positive and negative supercoils topological steps unity without requiring magnesium ions. is sensitive I-specific inhibitors like camptothecin berenil, unaffected by reagents novobiocin doxorubicin at II-inhibitory dosage. In presence...
The present research work was carried out at Navsari Agricultural University, during the summer 2014 to study correlation among quantitative traits and their direct indirect effect on seed yield in F2 populations (Meha X Pusa Vishal, Meha GM-4) of mungbean. Correlation analysis revealed that significantly positively correlated with pods per plants harvest index population Vishal plant height, primary branches plant, clusters straw GM-4. It indicates an association two characters is not only...