A5022765685- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Plant Pathogens and Fungal Diseases
- Plant Virus Research Studies
- Fermentation and Sensory Analysis
- Plant Parasitism and Resistance
- Toxin Mechanisms and Immunotoxins
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Resistance
- Bacteriophages and microbial interactions
- Fungal and yeast genetics research
- Mycorrhizal Fungi and Plant Interactions
- Plant pathogens and resistance mechanisms
- Yeasts and Rust Fungi Studies
- Plant Disease Resistance and Genetics
- Phytoplasmas and Hemiptera pathogens
- Agricultural safety and regulations
- Bioinformatics and Genomic Networks
- Protein Structure and Dynamics
- Pesticide Residue Analysis and Safety
- Polysaccharides and Plant Cell Walls
- Genomics, phytochemicals, and oxidative stress
- Pesticide Exposure and Toxicity
- Genetics, Bioinformatics, and Biomedical Research
- Fungal Biology and Applications
Sainsbury Laboratory
2022-2024
Norwich Research Park
2022-2024
National Institute of Agricultural Botany
2022-2024
Michigan State University
2024
University of East Anglia
2022-2023
East Malling Research (United Kingdom)
2015-2021
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2019
University of Reading
2018
University of Bath
2013-2014
Pathogen infection is a dynamic process. Here, we employ single-cell transcriptomics to investigate plant response heterogeneity. By generating an Arabidopsis thaliana leaf atlas encompassing 95,040 cells during by fungal pathogen, Colletotrichum higginsianum, unveil cell-type-specific gene expression, notably enrichment of intracellular immune receptors in vasculature cells. Trajectory inference identifies that had different interactions with the invading fungus. This analysis divulges...
Nicotinamide adenine dinucleotide (NAD + ) has emerged as a key component in prokaryotic and eukaryotic immune systems. The recent discovery that Toll/interleukin-1 receptor (TIR) proteins function NAD hydrolases (NADase) links -derived small molecules with signaling. We investigated pathogen manipulation of host metabolism virulence strategy. Using the pangenome model bacterial Pseudomonas syringae , we conducted structure-based similarity search from 35,000 orthogroups for type III...
Pathogens produce diverse effector proteins to manipulate host cellular processes. However, how functional diversity is generated in an repertoire poorly understood. Many effectors the devastating plant pathogen Phytophthora contain tandem repeats of "(L)WY" motif, which are structurally conserved but variable sequences. Here, we discovered a module formed by specific (L)WY-LWY combination multiple effectors, efficiently recruits serine/threonine protein phosphatase 2A (PP2A) core enzyme...
Summary Genome‐wide analyses of the effector‐ and toxin‐encoding genes were used to examine phylogenetics evolution pathogenicity amongst diverse strains Pseudomonas syringae causing bacterial canker cherry ( Prunus avium ), including pathovars P. pv morsprunorum Psm ) races 1 2, Pss avii . Phylogenetic revealed clades distinct each monophyletic, whereas cherry‐pathogenic interspersed from other host species. A maximum likelihood approach was predict effectors associated with on cherry....
Bacterial canker is a major disease of Prunus avium (cherry), domestica (plum) and other stone fruits. It caused by pathovars within the Pseudomonas syringae species complex including P. pv. morsprunorum (Psm) race 1 (R1), Psm 2 (R2) (Pss). R1 R2 were originally designated as same pathovar; however, phylogenetic analysis revealed them to be distantly related, falling into phylogroups 3 1, respectively. This study characterized pathogenicity 18 newly genome‐sequenced strains on cherry plum,...
Abstract When compared with other phylogroups (PGs) of the Pseudomonas syringae species complex, P. pv. s yringae (Pss) strains within PG2 have a reduced repertoire type III effectors (T3Es) but produce several phytotoxins. Effectors cherry pathogen Pss 9644 were grouped based on their frequency in from Prunus as conserved effector locus (CEL) common to most pathogens; core PG2; set PRUNUS and FLEXIBLE T3Es. also contains gene clusters for biosynthesis toxins syringomycin, syringopeptin...
Treating plant bacterial diseases is notoriously difficult because of the lack available antimicrobials. Pseudomonas syringae pathovar (Pss) a major pathogen cherry (Prunus avium) causing canker stem, leaf and fruit, impacting productivity leading to loss trees. In an attempt find treatment for this disease, naturally occurring bacteriophage (phage) that specifically target Pss being investigated as biocontrol strategy. However, before using them treatment, it important both understand their...
Pseudomonas syringae can cause stem necrosis and canker in a wide range of woody species including cherry, plum, peach, horse chestnut ash. The detection quantification lesion progression over time tissues is key trait for breeders to select upon resistance. In this study general, rapid reliable approach using image recognition an artificial neural network model was developed. This applied screen both the virulence P. pathovars resistance set cherry plum accessions bacterial canker. method...
Summary Many strains of Pseudomonas colonise plant surfaces, including the cherry canker pathogens, syringae pathovars and morsprunorum. We have examined genomic diversity P. in phyllosphere focused on role prophages transfer genes encoding Type 3 secreted effector (T3SE) proteins contributing to evolution virulence. Phylogenomic analysis was carried out epiphytic pseudomonads UK orchards. Significant differences populations occurred between regions. Nonpathogenic were found contain...
Fusarium oxysporum is a globally distributed soilborne fungal pathogen causing root rots, bulb crown rots and vascular wilts on range of horticultural plants. Pathogenic F. isolates are highly host specific classified as formae speciales. Narcissus an important ornamental crop both the quality yield flowers bulbs can be severely affected by basal rot caused f. sp. narcissi (FON); 154 were obtained from different locations cultivars in United Kingdom, representing valuable resource. A subset...
Bacterial canker is a major disease of stone fruits and critical limiting factor to sweet cherry (
The genus Saccharomyces comprises seven single-genome species (S. arboricola, S. cerevisiae, eubayanus, kudriavzevii, mikatae, paradoxus and uvarum) two hybrid - pastorianus cerevisiae plus eubayanus) bayanus (mostly uvarum eubayanus). Species-specific primers have already been developed for the identification of each species, these can usually detect both genomes in hybrids. It would be advantageous if a single reaction could any member clade. We investigated three potentially generic...
Fusarium proliferatum is a component of the onion basal rot disease complex. We present an annotated F. draft genome sequence, totaling 45.8 Mb in size, assembled into 597 contigs, with predicted 15,418 genes. The contains 58 secondary metabolite clusters and homologs oxysporum effector SIX2.
Abstract Nicotinamide adenine dinucleotide (NAD + ) has emerged as a key component in prokaryotic and eukaryotic immune systems the recent discovery that Toll/interleukin-1 receptor (TIR) proteins function NAD hydrolases (NADase) link -derived small molecules with signalling. We investigated pathogen manipulation of host metabolism virulence strategy. Using pangenome model bacterial Pseudomonas syringae, we conducted structure-based similarity search from 35,000 orthogroups for type III...
Abstract When compared with other phylogroups (PGs) of the Pseudomonas syringae (Ps) species complex, Ps pv. s yringae strains within PG2 have a reduced repertoire type III effectors (T3Es) but produce several phytotoxins. Effectors cherry pathogen Pss 9644 were grouped based on their frequency in from Prunus as: conserved effector locus (CEL) common to most pathogens; CORE PG2; set PRUNUS and FLEXIBLE T3Es. also contains gene clusters for biosynthesis toxins syringomycin/syringopeptin...
Abstract Background The plant pathogen Pseudomonas syringae ( Ps ) causes diseases in a broad range of hosts including important cash crops from several climate zones. In particular for woody crops, effective and environmentally friendly disease control strategies are not available. Although there is increasing evidence link between nutritional status susceptibility, optimization nutrition often neglected efforts to diseases. This review brings current knowledge on this topic together with...
Plant pathogenic Pseudomonas species naturally antagonize a diverse range of flowering plants. While emerging research demonstrates that isolates belonging to the P. syringae complex colonize hosts, extent which these bacteria infect non-flowering plants like model liverwort Marchantia polymorpha remains unclear. Here, we identify natural associations between viridiflava and polymorpha. isolated from diseased liverworts in wild successfully re-infected M. pure culture conditions, producing...
La phytopharmacovigilance (PPV) est un dispositif integratif mis en place par l’Anses dans le cadre de la loi d’avenir pour l’agriculture de 2014. Sa mission detecter les effets indesirables des produits phytopharmaceutiques la sante humaine et l’environnement. PPV repose sur trois piliers : un reseau partenaires gestionnaires systemes surveillance ou vigilance permettant de detecter signaux, declaration spontanee d’effets obligatoire acteurs professionnels etudes visant a consolider reseaux...
SUMMARY Pathogens produce diverse effector proteins to manipulate host cellular processes. However, how functional diversity is generated in an repertoire poorly understood. Many effectors the devastating plant pathogen Phytophthora contain tandem repeats of “(L)WY” motif, which are structurally conserved but variable sequences. Here, we discovered a module formed by specific (L)WY-LWY combination multiple effectors, efficiently recruit Serine/Threonine protein phosphatase 2A (PP2A) core...
Bacterial canker of Prunus, affecting economically important stone fruit crops including cherry, peach, apricot and plum, is caused by the plant pathogen Pseudomonas syringae (P.s.). Strains from two pathovars—P.s. pv. (Pss) P.s. morsprunorum race 1 (PsmR1) 2 (PsmR2)—in three phylogenetically distant clades have convergently evolved to infect Prunus. The bacteria enter woody tissues through wounds leaf scars, causing black necrotic cankers. Symptoms are also produced on blossom, leaves....