A5017609418- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Fungal and yeast genetics research
- Mycotoxins in Agriculture and Food
- Fungal Infections and Studies
- Fungal Biology and Applications
- Antifungal resistance and susceptibility
- Polysaccharides and Plant Cell Walls
- Entomopathogenic Microorganisms in Pest Control
- Polyamine Metabolism and Applications
- Fermentation and Sensory Analysis
- Plant Virus Research Studies
- Protein Hydrolysis and Bioactive Peptides
- Essential Oils and Antimicrobial Activity
- Nematode management and characterization studies
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Plant Pathogenic Bacteria Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Genomics, phytochemicals, and oxidative stress
- Seed and Plant Biochemistry
- Legume Nitrogen Fixing Symbiosis
- Antimicrobial Peptides and Activities
- Plant Disease Resistance and Genetics
- Insect Pest Control Strategies
Universidad de Guanajuato
2023-2024
Universidad Juárez del Estado de Durango
2021-2022
Universität Hamburg
2013-2020
University of Exeter
2013-2017
University of Córdoba
2004-2008
To cause plant diseases, pathogenic micro-organisms secrete effector proteins into host tissue to suppress immunity and support pathogen growth. Bacterial pathogens have evolved several distinct secretion systems target proteins, but whether fungi, which the major diseases of most crop species, also require different secretory mechanisms is not known. Here we report that rice blast fungus Magnaporthe oryzae possesses two effectors during infection. Cytoplasmic effectors, are delivered cells,...
Magnaporthe oryzae is the causal agent of rice blast disease, most devastating disease cultivated (Oryza sativa) and a continuing threat to global food security. To cause fungus elaborates specialized infection cell called an appressorium, which breaches cuticle leaf, allowing entry plant tissue. Here, we show that exocyst complex localizes tips growing hyphae during vegetative growth, ahead Spitzenkörper, required for polarized exocytosis. However, infection-related development,...
Hydrophobins (HPs) are small secreted fungal proteins possibly involved in several processes such as formation of aerial structures, attachment to hydrophobic surfaces, interaction with the environment and protection against host defense system. The genome necrotrophic plant pathogen Fusarium graminearum contains five genes encoding for HPs (FgHyd1-5). Single triple FgHyd mutants were produced characterized. A reduced growth was observed when ΔFghyd2 three including deletion FgHyd2 grown...
Abstract Fusarium graminearum is one of the most destructive plant pathogens worldwide, causing fusarium head blight (FHB) on cereals. F . colonizes wheat surfaces with specialized unbranched hyphae called runner (RH), which develop multicelled complex appressoria infection cushions (IC). IC generate multiple penetration sites, allowing fungus to enter cuticle. Complex structures are typical for several economically important pathogens, yet unknown molecular basis. In this study, RH and...
Rho-type GTPases regulate polarized growth in yeast by reorganization of the actin cytoskeleton and through signalling pathways that control expression cell wall biosynthetic genes. We report cloning functional analysis rho1 from Fusarium oxysporum, a soilborne fungal pathogen causing vascular wilt on plants opportunistic infections humans. F. oxysporum strains carrying either Δrho1 loss-of-function mutation or rho1G14V gain-of-function allele were viable, but displayed severely restricted...
Endo-polygalacturonases (PGs) and xylanases have been shown to play an important role during pathogenesis of some fungal pathogens dicot plants, while their in monocot is less defined. Pg1 xyr1 genes the wheat pathogen Fusarium graminearum encode main PG major regulator xylanase production, respectively. Single- double-disrupted mutants for these were obtained assess contribution infection. Compared with wild-type strain, ∆pg mutant showed a nearly abolished activity, slight reduced...
The establishment of polarity is a critical process in pathogenic fungi, mediating infection-related morphogenesis and host tissue invasion. Here, we report the identification TPC1 (Transcription factor for Polarity Control 1), which regulates invasive polarized growth rice blast fungus Magnaporthe oryzae. encodes putative transcription fungal Zn(II)2Cys6 family, exclusive to filamentous fungi. Tpc1-deficient mutants show severe defects conidiogenesis, infection-associated autophagy,...
Glycosylphosphatidylinositol-anchored (β)-1,3-glucanosyltransferases play active roles in fungal cell wall biosynthesis and morphogenesis have been implicated virulence on mammals. The role of β-1,3-glucanosyltransferases pathogenesis to plants has not explored so far. Here, we report the cloning mutational analysis gas1 gene encoding a putative β-1,3-glucanosyltransferase from vascular wilt fungus Fusarium oxysporum. In contrast Candida albicans, expression F. oxysporum was independent...
Cutinolytic enzymes are secreted by fungal pathogens attacking the aerial parts of plant, to facilitate penetration outermost cuticular barrier host. The role cutinases in soil-borne root has not been studied thus far. Here we report characterization zinc finger transcription factor Ctf1 from vascular wilt fungus Fusarium oxysporum, a functional orthologue CTF1alpha that controls expression cutinase genes and virulence pea stem pathogen solani f. sp. pisi. Mutants carrying Deltactf1...
Activation of eukaryotic translation initiation factor eIF5A requires a posttranslational modification, forming the unique amino acid hypusine. This activation is mediated by two enzymes, deoxyhypusine synthase, DHS, and hydroxylase, DOHH. The impact this enzymatic complex on life cycle fungal pathogen unknown. Plant pathogenic ascomycetes possess single copy activated hypusination. We evaluated importance imbalances in hypusination Fusarium graminearum, devastating cereals. Overexpression...
Upon posttranslational activation, the eukaryotic initiation factor-5A (eIF-5A) transports a subset of mRNAs out nucleus to ribosomes for translation. Activation protein is an evolutionary highly conserved process that unique eIF-5A, conversion lysine hypusine. Instrumental synthesis hypusine first two enzymatic reactions mediated by deoxyhypusine synthase (DHS). We show DHS wheat and pathogenic fungus Fusarium graminearum, which causes one most destructive crop diseases worldwide, are...
Food security is essential to ensure everyone can access sufficient nutritious food. Cereals and legumes are fundamental foods worldwide. Phaseolus vulgaris L., the common bean, an staple food in many nations However, it vulnerable fusariosis, a disease caused by fungus Fusarium spp. that significantly decrease crop quality yield. To combat plant diseases, industrial residues valuable due their bioactive compounds with biotechnological applications. This study proposes using ethanolic...
Sporothrix schenckii modulates the expression of its cell wall proteins (CWPs) in response to reactive oxygen species (ROS) generated by phagocytic cells human host, which allows it evade and escape immune system. In this study, we performed a comparative proteomic analysis CW S. after exposure nonexposure H2O2. Several CWPs involved remodeling fungal pathogenesis that modulated their oxidizing agent were identified, as number antioxidant enzymes atypical CWPs, called moonlighting proteins,...
The necrotrophic fungus F. graminearum is responsible for causing Fusarium head blight in wheat and ear rotin maize. This fungal infection leads to reduced crop yields contamination of the crops with mycotoxinslike trichothecene deoxynivalenol (DON), which can cause poisoning animals humans. productionof DON facilitated by a set genes, TRI5 gene encoding trichodiene synthase being key player.While some factors that activate are known, it's unclear whether this isactivated when infects or...