A5067716563- Metalloenzymes and iron-sulfur proteins
- Electrocatalysts for Energy Conversion
- Enzyme-mediated dye degradation
- Metal-Catalyzed Oxygenation Mechanisms
- Biofuel production and bioconversion
- Biochemical and biochemical processes
- Advanced battery technologies research
- Algal biology and biofuel production
- Microbial Metabolism and Applications
- Fungal and yeast genetics research
- Catalysis and Hydrodesulfurization Studies
- Trace Elements in Health
- RNA Research and Splicing
- Anaerobic Digestion and Biogas Production
- RNA and protein synthesis mechanisms
- Biodiesel Production and Applications
- Hydrogen Storage and Materials
- Fungal Biology and Applications
- Enzyme Catalysis and Immobilization
- Marine and coastal ecosystems
- Microbial metabolism and enzyme function
- Metal complexes synthesis and properties
- Plant-Microbe Interactions and Immunity
- Microbial Community Ecology and Physiology
- Enzyme Production and Characterization
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2016-2018
Universidad Politécnica de Madrid
2017-2018
Centre for Plant Biotechnology and Genomics
2016-2018
IMDEA Energy Institute
2007-2015
Energy Institute
2015
Madrid Institute for Advanced Studies
2010-2015
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
2007-2008
Institut National des Sciences Appliquées de Toulouse
2007-2008
Centre National de la Recherche Scientifique
2007-2008
SPring-8
2008
Surprisingly uninhibited: The inhibition of hydrogenases by oxygen is intensely studied because this the main obstacle to using these enzymes in biofuel cells. hydrogenase from Clostridium acetobutylicum (see structure) was found react surprisingly slowly with O2. mechanism elucidated and kinetics were quantitatively defined. This a prerequisite for improving enzyme further genetic engineering assessing its potential technological devices. Supporting information article available on WWW...
The establishment of polarity is a critical process in pathogenic fungi, mediating infection-related morphogenesis and host tissue invasion. Here, we report the identification TPC1 (Transcription factor for Polarity Control 1), which regulates invasive polarized growth rice blast fungus Magnaporthe oryzae. encodes putative transcription fungal Zn(II)2Cys6 family, exclusive to filamentous fungi. Tpc1-deficient mutants show severe defects conidiogenesis, infection-associated autophagy,...
In Clostridium acetobutylicum, [FeFe]-hydrogenase is involved in hydrogen production vivo by transferring electrons from physiological electron donors, ferredoxin and flavodoxin, to protons. this report, modifications of the purification procedure, specific activity enzyme has been improved its complete catalytic profile evolution, uptake, proton/deuterium exchange para-H2/ortho-H2 conversion determined. The major expressed solvent-producing C. acetobutylicum cells was purified identified as...
NifB-co, an Fe-S cluster produced by the enzyme NifB, is intermediate on biosynthetic pathway to iron molybdenum cofactor (FeMo-co) of nitrogenase. We have used Fe K-edge extended X-ray absorption fine structure (EXAFS) spectroscopy together with (57)Fe nuclear resonance vibrational (NRVS) probe NifB-co while bound NifX protein from Azotobacter vinelandii. The spectra been interpreted in part comparison data for completed FeMo-co attached NafY carrier protein: NafY:FeMo-co complex. EXAFS...
The presence of inhibitors compounds after pretreatment lignocellulosic materials affects the saccharification and fermentation steps in bioethanol production processes. Even though, external addition laccases selectively removes phenolic from prehydrolysates, when it is coupled to step, lower hydrolysis yields are attained. Vanillin, syringaldehyde ferulic acid commonly found wheat-straw prehydrolysate steam-explosion pretreatment. These three were used this study elucidate inhibitory...
The biological activation of N2 occurs at the FeMo-cofactor, a 7Fe-9S-Mo-C-homocitrate cluster. FeMo-cofactor formation involves assembly Fe6-8 -SX -C core precursor, NifB-co, which on NifB protein. Characterization NifB-co in is complicated by dynamic nature process and presence permanent [4Fe-4S] cluster associated with radical SAM chemistry for generating central carbide. We have used physiological carrier protein, NifX, has been proposed to bind deliver it NifEN upon ultimately...
NafY participates in the final steps of nitrogenase maturation, having a dual role as iron-molybdenum cofactor (FeMo-co) carrier and chaperone to FeMo-co-deficient apo-NifDK (apo-dinitrogenase). contains an N-terminal domain unknown function (n-NafY) C-terminal (core-NafY) necessary for FeMo-co binding. We show here that n-NafY core-NafY have very weak interactions intact NafY. The NMR structure reveals it belongs sterile α-motif (SAM) family domains, which are frequently involved...
Summary Generation of mRNA isoforms by alternative polyadenylation ( APA ) and their involvement in regulation fungal cellular processes, including virulence, remains elusive. Here, we investigated genome‐wide site PAS selection the rice blast fungus to understand how regulates pathogenicity. More than half Magnaporthe oryzae transcripts undergo show novel motifs region. Transcripts with shorter 3′ UTR s are more stable abundant polysomal fractions, suggesting they being translated...
Zögerliche Inhibition: Die Inhibition von Hydrogenasen durch Sauerstoff stellt das Haupthindernis bei der Anwendung dieser Enzyme in Biobrennstoffzellen dar. Hydrogenase aus Clostridium acetobutylicum (siehe Struktur) reagiert überraschend langsam mit O2; zugehörige Inhibitionsmechanismus wurde aufgeklärt und die Kinetik des Prozesses quantifiziert. Auf Grundlage kann Enzym nun gentechnisch verbessert werden, es wird möglich, sein Anwendungspotenzial einzuschätzen. Supporting information for...
Abstract The biological activation of N 2 occurs at the FeMo‐cofactor, a 7Fe–9S–Mo–C–homocitrate cluster. FeMo‐cofactor formation involves assembly Fe 6–8 –S X –C core precursor, NifB‐co, which on NifB protein. Characterization NifB‐co in is complicated by dynamic nature process and presence permanent [4Fe–4S] cluster associated with radical SAM chemistry for generating central carbide. We have used physiological carrier protein, NifX, has been proposed to bind deliver it NifEN upon...
Abstract The addition of laccase enzymes reduces the amount phenols present in lignocellulosic pretreated materials and increases their fermentability. However, combination with cellulases hydrolysis yields. In this work, hybrid fermentation (HHF) configuration allowed overcoming negative effect treatment on enzymatic hydrolysis. Furthermore, effects different dosages, length detoxification time inoculum size ethanol production were evaluated. evaluated configurations, dosages did not show...