A5007419110- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Fungal and yeast genetics research
- Plant Pathogens and Fungal Diseases
- Legume Nitrogen Fixing Symbiosis
- Plant Disease Resistance and Genetics
- Plant Parasitism and Resistance
- Plant biochemistry and biosynthesis
- Plant pathogens and resistance mechanisms
- GABA and Rice Research
- Mycorrhizal Fungi and Plant Interactions
- Microbial Natural Products and Biosynthesis
- Wheat and Barley Genetics and Pathology
- Mycotoxins in Agriculture and Food
- Plant and Fungal Interactions Research
- Biofuel production and bioconversion
- Plant Virus Research Studies
- RNA Research and Splicing
- Nematode management and characterization studies
- Cancer-related molecular mechanisms research
- Gene expression and cancer classification
- Force Microscopy Techniques and Applications
- Biochemical and biochemical processes
- 14-3-3 protein interactions
- RNA and protein synthesis mechanisms
Universidad Politécnica de Madrid
2011-2018
Centre for Plant Biotechnology and Genomics
2014-2018
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2016-2018
John Innes Centre
2005-2017
University of Münster
2007
CeNTech
2007
University Hospital Münster
2007
Sainsbury Laboratory
2004-2005
SIB Swiss Institute of Bioinformatics
2005
Universidad Publica de Navarra
1998-2002
Glomalean fungi induce and colonize symbiotic tissue called arbuscular mycorrhiza on the roots of most land plants. Other also plants but cause disease not symbiosis. Whole-transcriptome analysis using a custom-designed Affymetrix Gene-Chip confirmation with real-time RT-PCR revealed 224 genes affected during mycorrhizal We compared these transcription profiles those from rice that were colonized by pathogens ( Magnaporthe grisea Fusarium moniliforme ). Over 40% showed differential...
The 154-kb plasmid was cured from race 7 strain 1449B of the phytopathogen Pseudomonas syringae pv. phaseolicola ( Pph ). Cured strains lost virulence toward bean, causing hypersensitive reaction in previously susceptible cultivars. Restoration achieved by complementation with cosmid clones spanning a 30-kb region that contained identified avirulence avr ) genes avrD , avrPphC and avrPphF . Single transposon insertions at multiple sites (including one located abolished restoration genomic...
• There is controversy as to whether specific natural products play a role in directly mediating antagonistic plant–plant interactions – that is, allelopathy. If proved exist, such phenomena would hold considerable promise for agronomic improvement of staple food crops rice (Oryza sativa). However, while substantiated by the presence phytotoxic compounds at potentially relevant concentrations, demonstrating direct allelopathy has been difficult because chemical complexity root and plant...
Among their responses to microbial infection, plants deploy an arsenal of natural antibiotic products. Historically these have been identified on the basis activity in vitro, which leaves open question relevance defense planta. The vast majority such products from important crop plant rice (Oryza sativa) are diterpenoids whose biosynthesis proceeds via either ent- or syn-copalyl diphosphate (CPP) intermediates, were isolated against fungal blast pathogen Magnaporthe oryzae. However, gene for...
RNA-binding proteins play a central role in post-transcriptional mechanisms that control gene expression. Identification of novel fungi is essential to unravel networks and cellular processes confer identity the fungal kingdom. Here, we carried out functional characterisation filamentous fungus-specific protein RBP35 required for full virulence development rice blast fungus. contains an N-terminal RNA recognition motif (RRM) six Arg-Gly-Gly tripeptide repeats. Immunoblots identified two...
The establishment of polarity is a critical process in pathogenic fungi, mediating infection-related morphogenesis and host tissue invasion. Here, we report the identification TPC1 (Transcription factor for Polarity Control 1), which regulates invasive polarized growth rice blast fungus Magnaporthe oryzae. encodes putative transcription fungal Zn(II)2Cys6 family, exclusive to filamentous fungi. Tpc1-deficient mutants show severe defects conidiogenesis, infection-associated autophagy,...
ABSTRACT Bacterial apical necrosis of mango, elicited by Pseudomonas syringae pv. syringae, limits fruit production in southern Spain and Portugal. Examination a collection P. isolates for copper resistance showed that 59% were resistant to cupric sulfate. The survey mango orchard revealed an increase frequencies copper-resistant bacteria after repeated treatments with Bordeaux mixture. These data suggest selection strains could be major reason control failures following management...
Abstract Magnaporthe oryzae is the most important fungal pathogen of rice (Oryza sativa). Under laboratory conditions, it able to colonize both aerial and underground plant organs using different mechanisms. Here, we characterize an infection-related development in M. produced on hydrophilic polystyrene (PHIL-PS) roots. We show that spores develop preinvasive hyphae (pre-IH) from hyphopodia (root penetration structures) or germ tubes pre-IH also enter root cells. Changes cell wall structure...
Pathogen-Host interaction data is core to our understanding of disease processes and their molecular/genetic bases. Facile access such particularly important for the plant sciences, where individual genetic phenotypic observations have added complexity being dispersed over a wide diversity species versus relatively fewer host interest biomedical researchers. Recently, an international initiative interested in scholarly publishing proposed that all scientific should be "FAIR" - Findable,...
Pseudomonas syringae pv. phaseolicola ( Pph ) race 4 strain 1302A carries avirulence gene avrPphB . Strain RJ3, a sectoral variant from culture, exhibited an extended host range in cultivars of bean and soybean resulting the absence RJ3 chromosome. Complementation with restored phenotype. Both strains showed similar planta growth susceptible cultivars. Analysis indicated loss > 40 kb DNA surrounding Collinearity two genomes was determined for left right junctions deleted region; junction...
Linoleate diol synthases (LDS) are heme enzymes, which oxygenate 18:2n-6 sequentially to (8R)-hydroperoxylinoleic acid ((8R)-HPODE) and (5S,8R)-dihydroxy-, (7S,8S)-dihydroxy-, or (8R,11S)-dihydroxylinoleic acids (DiHODE). The genome of the rice blast fungus, Magnaporthe oryzae, contains two genes with homology LDS. M. oryzae oxidized (8R)-HPODE (7S,8S)-DiHODE, (6S,8R)-DiHODE, (8R,11S)-HODE. Small amounts 10-hydroxy-(8E,12Z)-octadecadienoic traces 5,8-DiHODE were also detected by liquid...
Summary virPphA is a major determinant of the pathogenicity Pseudomonas savastanoi pv. phaseolicola to Phaseolus bean. A family homologues was detected in pathovars P. and syringae. We examined structure activity alleles designated virPphA, virPphA(Pgy), virPphA(Psv) from phaseolicola, glycinea, savastanoi, respectively, avrPtoB syringae tomato. Sequencing showed that virPphA(Pgy) homologue had 48-bp central deletion open reading frame (ORF) compared with virPphA(Psv), but otherwise all...
ABSTRACT Many Pseudomonas syringae strains contain native plasmids that are important for host-pathogen interactions, and most of them several coexisting (pPT23A-like plasmids) cross-hybridize to replication sequences from pPT23A, which also carries a gene cluster coding the phytotoxin coronatine in P. pv. tomato PT23. In this study, three functional pPT23A-like replicons were cloned glycinea race 6, suggesting compatibility highly related is common feature strains. Hybridization experiments...
Topographical features are known to impose capillary forces on liquid droplets, and this phenomenon is exploited in applications such as printing, coatings, textiles microfluidics. Surface topographies also influence the behavior of biological cells (i.e., contact guidance), with implications ranging from medicine agriculture. An accurate physical description how detect respond surface necessary order move beyond a purely heuristic approach optimizing biomaterial interfaces. Here, we have...
Summary: Many strains of the phytopathogen Pseudomonas syringae contain mutually compatible plasmids that share extensive regions sequence homology and essential replication determinants. The two large involved in virulence or pathogenicity, pPT23A from P. pv. tomato strain PT23 pAV505 phaseolicola HRI1302A, were isolated. DNA sequencing origins revealed homologous ORFs, designated ORF-Pto ORF-Pph, respectively. Both ORFs are 1311 bp long encode peptides 437 amino acids with predicted...
It was previously shown that most Pseudomonas syringae strains contain one or more plasmids with cross-hybridizing replication regions and other areas of homology, these were designated the pPT23A-like family. The majority encode genes conferring epiphytic fitness resistance to antibacterial compounds those investigated in this study are essential for pathogenicity increased virulence. phylogeny 14 from five P. pathovars studied by comparing a fragment sequence their repA (encoding replicase...
Summary Generation of mRNA isoforms by alternative polyadenylation ( APA ) and their involvement in regulation fungal cellular processes, including virulence, remains elusive. Here, we investigated genome‐wide site PAS selection the rice blast fungus to understand how regulates pathogenicity. More than half Magnaporthe oryzae transcripts undergo show novel motifs region. Transcripts with shorter 3′ UTR s are more stable abundant polysomal fractions, suggesting they being translated...
Cleavage factor I (CFI) proteins are core components of the polyadenylation machinery that can regulate several steps mRNA life cycle, including alternative polyadenylation, splicing, export and decay. Here, we describe regulatory mechanisms control two fungal CFI protein classes in Magnaporthe oryzae: Rbp35/CfI25 complex Hrp1. Using mutational, genetic biochemical studies demonstrate cellular concentration mRNAs is a limited indicator their abundance. Our results suggest...