A5080242864- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- SARS-CoV-2 detection and testing
- Pharmaceutical and Antibiotic Environmental Impacts
- Plant pathogens and resistance mechanisms
- Yersinia bacterium, plague, ectoparasites research
- COVID-19 diagnosis using AI
- Protein Structure and Dynamics
- Bacterial Genetics and Biotechnology
- Bacterial Identification and Susceptibility Testing
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Animal Ecology and Behavior Studies
- COVID-19 epidemiological studies
- Plant Pathogens and Fungal Diseases
- Banana Cultivation and Research
- Respiratory viral infections research
- Phytoplasmas and Hemiptera pathogens
- Probiotics and Fermented Foods
- Antimicrobial Resistance in Staphylococcus
- Insect and Pesticide Research
- RNA and protein synthesis mechanisms
University of Bath
2020-2024
University of the West of England
1996-2001
Wye College
1999
Universidad Publica de Navarra
1999
Max Delbrück Center
1992
Abstract The Klebsiella group, found in humans, livestock, plants, soil, water and wild animals, is genetically ecologically diverse. Many species are opportunistic pathogens can harbour diverse classes of antimicrobial resistance genes. Healthcare-associated pneumoniae clones that non-susceptible to carbapenems spread rapidly, representing a high public health burden. Here we report an analysis 3,482 genome sequences 15 sampled over 17-month period from wide range clinical, community,...
The 154-kb plasmid was cured from race 7 strain 1449B of the phytopathogen Pseudomonas syringae pv. phaseolicola ( Pph ). Cured strains lost virulence toward bean, causing hypersensitive reaction in previously susceptible cultivars. Restoration achieved by complementation with cosmid clones spanning a 30-kb region that contained identified avirulence avr ) genes avrD , avrPphC and avrPphF . Single transposon insertions at multiple sites (including one located abolished restoration genomic...
Antibacterial resistance (ABR) is a major global health security threat, with disproportionate burden on lower-and middle-income countries (LMICs). It not understood how 'One Health', where human co-dependent animal and the environment, might impact of ABR in LMICs. Thailand's 2017 "National Strategic Plan Antimicrobial Resistance" (NSP-AMR) aims to reduce AMR morbidity by 50% through 20% reductions 30% antibacterial use (ABU). There need understand implications such plan within One Health...
We showed that a bacterial avirulence (avr) gene function, avrPpiA1, from the pea pathogen Pseudomonas syringae pv pisi, is recognized by some, but not all, genotypes of Arabidopsis. Thus, an avr functionally defined on crop species also The activity avrPpiA1 series Arabidopsis identical to avrRpm1 P.s. maculicola previously using two genes are homologous and encode nearly predicted products. Moreover, this conserved function some bean cultivars in what has been shown be gene-for-gene...
Abstract Bacteria closely control gene expression to ensure optimal physiological responses their environment. Such careful can minimize the fitness cost associated with antibiotic resistance. We previously described a novel regulatory logic in Bacillus subtilis enabling cell directly monitor its need for detoxification. This cost‐effective strategy is achieved via two‐component system (BceRS) working sensory complex an ABC‐transporter (BceAB), together acting as flux‐sensor where signaling...
Researchers around the world have demonstrated correlations between measurements of SARS-CoV-2 RNA in wastewater (WW) and case rates COVID-19 derived from direct testing individuals. This has raised concerns that wastewater-based epidemiology (WBE) methods might be used to quantify spread this other diseases, perhaps faster than testing, with less expense intrusion. We illustrate, using data Scotland USA, issues regarding construction effective predictive models for disease rates. discuss...
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Abstract The Klebsiella group is highly diverse both genetically and ecologically, being commonly recovered from humans, livestock, plants, soil, water, wild animals. Many species are opportunistic pathogens, can harbour classes of antimicrobial resistance (AMR) genes. K. pneumoniae responsible for a high public-health burden, due in part to the rapid spread health-care associated clones that non-susceptible carbapenems. thus represents pertinent taxon assessing risk public health posed by...
The increasing availability of whole genome sequencing bacteria has accelerated the discovery novel species which may not have been easy to discriminate using standard phenotypic or single gene methods. Phylogenomic analysis sequences from a collection coagulase-negative staphylococcal isolated captive fruit bats revealed two clusters were close Staphylococcus kloosii . To assess relatedness strains we used digital DNA–DNA hybridization (dDDH) and methods for average nucleotide identity...
Abstract Background Klebsiella pneumoniae (Kp) is an important pathogen of humans and animals, recent reports ‘convergent’ strains that carry both virulence antimicrobial resistance genes (ARGs) have raised serious public health concern. The plasmid-borne iuc locus, encoding the siderophore aerobactin, a key factor in this species. variant 3 associated with porcine human clinical isolates carried by mostly uncharacterised IncF plasmids. Methods We used combination short-read long-read...
Klebsiella species occupy a wide range of environmental and animal niches, occasionally cause opportunistic infections that are resistant to multiple antibiotics. In particular, pneumoniae ( Kpne ) has gained notoriety as major nosocomial pathogen, due principally the rise in non-susceptibility carbapenems other beta-lactam Whilst it been proposed urban water cycle facilitates transmission pathogens between clinical settings environment, level risk posed by strains hospital wastewater...
Summary: Many strains of the phytopathogen Pseudomonas syringae contain mutually compatible plasmids that share extensive regions sequence homology and essential replication determinants. The two large involved in virulence or pathogenicity, pPT23A from P. pv. tomato strain PT23 pAV505 phaseolicola HRI1302A, were isolated. DNA sequencing origins revealed homologous ORFs, designated ORF-Pto ORF-Pph, respectively. Both ORFs are 1311 bp long encode peptides 437 amino acids with predicted...
An avirulence gene (designated avrPpiB) from race 3 of Pseudomonas syringae pv. pisi was cloned and sequenced. The corresponded to a single open reading frame 831 nt identified by transposon mutagenesis subcloning. This ORF encodes predicted hydrophilic protein 276 amino acids (MW 31,300). It effects the expression resistance mechanism governed genetic locus in pea. Cosegregation at R3 pea observed towards transconjugant carrying avrPpiB according 3:1 ratio resistant:susceptible F2 progeny...
Fatal exudative dermatitis (FED) is a significant cause of death red squirrels ( Sciurus vulgaris ) on the island Jersey in Channel Islands where it associated with virulent clone Staphylococcus aureus, ST49. S. aureus ST49 has been found other hosts such as small mammals, pigs and humans, but dynamics carriage disease this clone, or any lineage squirrels, currently unknown. We used whole-genome sequencing to characterize 228 isolates from healthy Jersey, Isle Arran (Scotland) Brownsea...
Conservation of endangered species has become increasingly complex, and costly interventions to protect wildlife require a robust scientific evidence base. This includes consideration the role microbiome in preserving animal health. Captivity introduces stressors not encountered wild including environmental factors exposure exotic species, humans antimicrobial drugs. These may perturb microbiomes animals, with negative consequences for their health welfare hence success conservation project,...
The rise of antimicrobial resistance (AMR) in bacterial pathogens such as Klebsiella pneumoniae (Kp) is a pressing public health and economic concern. 'One-Health' framework recognizes that effective management AMR requires surveillance agricultural well clinical settings, particularly low-resource regions Pakistan. Here, we use whole-genome sequencing to characterise 49 isolates Klebisella spp. (including 43 Kp) 2 presumptive Providencia rettgeri recovered from dairy farms located near 3...
Introduction The dissemination of antimicrobial resistance (AMR) in critical priority pathogens is a significant threat. Non-clinical reservoirs AMR, such as agriculture and food production facilities, may contribute to the transmission clinically relevant multidrug-resistant (MDR) Klebsiella pneumoniae . There currently very limited knowledge regarding population structure genomic diversity K. poultry Pakistan. Methods We explored healthy broilers commercial farm from Faisalabad, Pakistan,...
SUMMARY Bacteria closely control gene expression to ensure optimal physiological responses their environment. Such careful can minimize the fitness cost associated with antibiotic resistance. We previously described a novel regulatory logic in Bacillus subtilis enabling cell directly monitor its need for detoxification. This cost-effective strategy is achieved via two-component system (BceRS) working sensory complex an ABC-transporter (BceAB), together acting as flux-sensor where signaling...
During a citywide microbiological screening project in Pavia (Italy) bacterial strain isolated from the surface of an Automated Teller Machine was classified as Klebsiella sp. by MALDI-TOF spectrometry, and shown to be susceptible most antimicrobial classes phenotypic testing. After Illumina genome sequencing subsequent assembly, high-quality draft obtained (size = 5,051,593 bp, N50 615,571 largest contig 1,328,029 N_contig 17, GC content 51.58%, coverage 141.42), absence resistance genes...