A5079793068- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Genomics and Phylogenetic Studies
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Nosocomial Infections in ICU
- Gut microbiota and health
- Bacteriophages and microbial interactions
- Pneumonia and Respiratory Infections
- Sepsis Diagnosis and Treatment
- Plant Pathogenic Bacteria Studies
- Neonatal and Maternal Infections
- Enterobacteriaceae and Cronobacter Research
- Streptococcal Infections and Treatments
- Pharmaceutical and Antibiotic Environmental Impacts
- Heart Failure Treatment and Management
- Antibiotics Pharmacokinetics and Efficacy
- Medication Adherence and Compliance
- Escherichia coli research studies
- Urinary Tract Infections Management
- Salmonella and Campylobacter epidemiology
- Chronic Disease Management Strategies
- Liver Disease Diagnosis and Treatment
- Infections and bacterial resistance
Policlinico San Matteo Fondazione
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2016-2025
Bellvitge University Hospital
2023-2024
Institut d'Investigació Biomédica de Bellvitge
2023
University of Ottawa
2023
University of Girona
2023
University of Pavia
2014-2020
Foundation Center
2017
Duran i Reynals Hospital
1997
Abstract The Klebsiella group, found in humans, livestock, plants, soil, water and wild animals, is genetically ecologically diverse. Many species are opportunistic pathogens can harbour diverse classes of antimicrobial resistance genes. Healthcare-associated pneumoniae clones that non-susceptible to carbapenems spread rapidly, representing a high public health burden. Here we report an analysis 3,482 genome sequences 15 sampled over 17-month period from wide range clinical, community,...
Klebsiella oxytoca causes opportunistic human infections and post-antibiotic haemorrhagic diarrhoea. This Enterobacteriaceae species is genetically heterogeneous currently subdivided into seven phylogroups (Ko1 to Ko4, Ko6 Ko8). Here we investigated the taxonomic status of Ko3 Ko4. Genomic sequence-based phylogenetic analyses demonstrate that Ko4 formed well-defined sequence clusters related to, but distinct from, michiganensis (Ko1), (Ko2), K. huaxiensis (Ko8) grimontii (Ko6). The average...
One Health approaches to address the increasing threat of antimicrobial resistance (AMR) are gaining attention. However, data on distribution and movement bacteria their AMR-associated genes between clinical non-clinical sources scarce, especially from low-income middle-income countries. We aimed analyse Klebsiella isolates various in Ghana compare prevalence AMR with datasets two other
ABSTRACT Hypervirulent Klebsiella pneumoniae (hvKp) can cause infections in clinically healthy people, such as young and immunocompetent patients. Genes involved the capsule synthesis or those encoding siderophores have been adopted predictors of hvKp. Certain sequence types, ST23 ST86, associated with hvKp strains, too. The aim this study was to investigate presence among 354 K. strains isolated from clinical samples patients admitted an Italian 900-bed hospital between 21 May 2021 April...
Klebsiella pneumoniae is at the forefront of antimicrobial resistance for Gram-negative pathogenic bacteria, as strains resistant to third-generation cephalosporins and carbapenems are widely reported. The worldwide diffusion these great concern due high morbidity mortality often associated with K. infections in nosocomial environments. We sequenced genomes 89 isolated six Italian hospitals. Strains were selected based on antibiotypes, regardless multilocus sequence type, obtain a picture...
The dissemination of carbapenemase-producing Escherichia coli, although still at low level, should be continuously monitored. OXA-244 is emerging in Europe, mainly E. coli . In Italy, this carbapenemase was reported from an environmental river sample 2019. We report clinical isolates OXA-244-producing ST131 four patients admitted to acute care hospital Pavia, Italy. association difficult-to-detect determinant with a globally circulating high-risk clone, relevance.
Coagulase-negative staphylococci (CoNS) are commensal on human body surfaces and, for years, they were not considered a cause of bloodstream infection and often regarded as contamination. However, the involvement CoNS in nosocomial is increasingly being recognized. The insertion cannulas intravascular catheters represents primary source entry into bloodstream, causing bacteremia sepsis. They owe their pathogenic role to ability produce biofilms surfaces, such medical devices. In this study,...
During 2022 and 2023, a large upsurge of cases Group A Streptococcus (GAS) infection has struck many countries worldwide. This epidemic event was caused by diminished level immunity after COVID-19 social restrictions fostered mainly the emm1 genotype bacterium. We characterized genomes GAS isolates that bloodstream infections in last ten years 900-bed hospital Northern Italy. obtained short-read genomes, which we used for emm typing to analyze antimicrobial resistance virulence gene content....
<title>Abstract</title> Background While antimicrobial susceptibility testing (AST) isn’t routinely performed for <italic>C. difficile</italic> infection (CDI), reports of resistance have increased in surveillance studies. The aim this study was to assess the rate four antimicrobials (vancomycin, metronidazole, tigecycline and ciprofloxacin), risk factors evaluate MIC variation patients with recurrence. Methods Data from consecutive CDI admitted our Istitution between 1st January 2022 30th...
The spread of extended-spectrum beta-lactamase (ESBL)- and carbapenemase-producing Escherichia coli clones in humans, animals, the environment is great concern worldwide. In this study, we characterized four E. (CP-Ec) isolated from human samples. Two isolates ST401, rarely associated with carbapenemase and/or ESBL resistance genes, harbored blaKPC-3 blaVIM-1 respectively, were genetically distant each other. One CP-Ec isolate belonging to ST355, typically found poultry environmental sources...
Abstract Background Infections occurring in the early post–heart transplant (HT) period heavily contribute to morbidity and mortality. Our goal is evaluate incidence of hospital‐acquired pneumonia/ventilator‐associated pneumonia (HAP/VAPs) and/or bloodstream infections (BSIs) after HT during intensive care unit (ICU) stay identify their associated risk factors our tertiary hospital. Methods Observational prospective study including all adult patients who consecutively underwent from January...
Abstract Introduction multidrug resistant microorganisms(MDROs), once nearly exclusive of hospitalized patients, can now be found in the community. Few studies exist on their prevalence long term care facilities (LTCFs), despite fact that they likely are important reservoirs, given residents' need for continuous assistance, medical devices, hospitalisation, and antibiotics. This study establishes MDROs LTCFs province Pavia, an area Northern Italy 3000 km2 more than 500 000 inhabitant. Risk...
A correlation between resident non-pathogenic bacterial populations in certain natural mineral waters and their beneficial effects has been established by several research groups. This study aims to characterize the composition of Rivanazzano salso-bromo-jodic sulphurous (Pavia, Italy). Water samples were collected from sources dispensing systems. DNA was extracted subjected 16S rRNA gene sequencing. Microbial composition, as well alpha beta diversity, analyzed using amplicon sequence...
The circulation of carbapenem-resistant Klebsiella pneumoniae (CRKP) is a significant problem worldwide. In this work we characterize the isolates and reconstruct spread multi-clone epidemic event that occurred in an Intensive Care Unit hospital Northern Italy. took place from August 2015 to May 2016 involved 23 patients. Twelve these patients were colonized by CRKP at gastrointestinal level, while other 11 infected various body districts. We retrospectively collected data on inpatients...
Abstract Bacterial species often comprise well-separated lineages, likely emerged and maintained by genetic isolation and/or ecological divergence. How these two evolutionary actors interact in the shaping of bacterial population structure is currently not fully understood. In this study, we investigate drivers underlying evolution Serratia marcescens , an opportunistic pathogen with high genomic flexibility able to colonise diverse environments. Comparative analyses reveal a composed five...
Erysipelothrix rhusiopathiae is a facultative anaerobe Gram-positive bacillus, which considered zoonotic pathogen. E. causes erysipeloid, mainly in occupational groups such as veterinarians, slaughterhouse workers, farmers, and fishermen. Two cutaneous forms (localised generalised) septicaemic form have been described. Here, we report the isolation of strain from 56-year-old immunocompetent obese male admitted to Fondazione IRCCS Policlinico San Matteo Pavia (Italy). Blood cultures were...
We describe three cases of bloodstream infection caused by colistin-resistant Escherichia coli in patients a tertiary hospital Italy, between August 2016 and January 2017. Whole genome sequencing detected the mcr-1 gene isolated strains belonging to different sequence types (STs). This occurrence with -positive E. STs six months suggests widespread problem settings where high multidrug resistance is endemic such as Italy.
Abstract The Klebsiella group is highly diverse both genetically and ecologically, being commonly recovered from humans, livestock, plants, soil, water, wild animals. Many species are opportunistic pathogens, can harbour classes of antimicrobial resistance (AMR) genes. K. pneumoniae responsible for a high public-health burden, due in part to the rapid spread health-care associated clones that non-susceptible carbapenems. thus represents pertinent taxon assessing risk public health posed by...
Acinetobacter baumannii is a nosocomial pathogen that poses serious threat due to the rise of incidence multidrug-resistant (MDR) strains. During COVID-19 pandemic, MDR A. clones have caused several outbreaks worldwide. Here, we describe detailed investigation an outbreak occurred at Policlinico San Matteo (Pavia, Italy). A total 96 strains, isolated between January and July 2020 from 41 inpatients (both SARS-CoV-2 positive negative) in different wards, were characterized by phenotypic...
The Comano spring water (Comano, Italy) has been demonstrated to improve skin regeneration, not only by increasing keratinocyte proliferation and migration, but also modulating the regenerated collagen elastic fibers in dermis. However, such biological properties may be entirely explained its mineral composition only. As non-pathogenic bacterial populations have an active role different processes, potential presence of species within was investigated order identify any possible correlation...
Abstract Background Klebsiella pneumoniae (Kp) is an important pathogen of humans and animals, recent reports ‘convergent’ strains that carry both virulence antimicrobial resistance genes (ARGs) have raised serious public health concern. The plasmid-borne iuc locus, encoding the siderophore aerobactin, a key factor in this species. variant 3 associated with porcine human clinical isolates carried by mostly uncharacterised IncF plasmids. Methods We used combination short-read long-read...
Minimum Inhibitory Concentrations (MICs) are the gold standard for quantitatively measuring antibiotic resistance. However, lab-based MIC determination can be time-consuming and suffers from low reproducibility, interpretation as sensitive or resistant relies on guidelines which change over time. Genome sequencing machine learning promise to allow in silico prediction an alternative approach overcomes some of these difficulties, albeit is still needed. Nevertheless, precisely how we should...