A5011080630- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotics Pharmacokinetics and Efficacy
- Enterobacteriaceae and Cronobacter Research
- Vibrio bacteria research studies
- Antibiotic Use and Resistance
- Mycobacterium research and diagnosis
- Urinary Tract Infections Management
- Escherichia coli research studies
- Antimicrobial Resistance in Staphylococcus
- Infections and bacterial resistance
- Bacterial Identification and Susceptibility Testing
- Bacterial Genetics and Biotechnology
- Tuberculosis Research and Epidemiology
- Probiotics and Fermented Foods
- Neonatal and Maternal Infections
- Nosocomial Infections in ICU
- Cancer therapeutics and mechanisms
- Plant Pathogenic Bacteria Studies
- Medical Device Sterilization and Disinfection
- Streptococcal Infections and Treatments
- Enzyme Production and Characterization
- Antifungal resistance and susceptibility
- Pneumonia and Respiratory Infections
University of Udine
2021-2024
Guglielmo da Saliceto Hospital
2021-2024
University of Pavia
2010-2020
Radboud University Nijmegen
2018
Radboud University Medical Center
2018
Istituto Giannina Gaslini
2016
Ospedale di Bolzano
2014-2015
Centre Hospitalier Annecy Genevois
2015
Foundation Center
2013-2014
Laboratoire d’Etudes du Rayonnement et de la Matière en Astrophysique et Atmosphères
2014
Twelve isolates of Enterobacteriaceae (1 Klebsiella pneumoniae, 8 Escherichia coli, 1 Proteus mirabilis, and 2 vulgaris) classified as extended-spectrum beta-lactamase (ESBL) producers according to the ESBL screen flow application BD-Phoenix automatic system for which cefotaxime MICs were higher than those ceftazidime collected between January 2001 July 2002 at Laboratory Clinical Microbiology San Matteo University Hospital Pavia (northern Italy). By PCR sequencing, a CTX-M-type determinant...
Carbapenem-resistant Enterobacteriaceae (CRE) are emerging as a public health problem in various settings. In Italy, rapid and remarkable increase of carbapenem-non-susceptible Klebsiella pneumoniae has been reported since 2010. Here we report on the results countrywide cross-sectional survey, carried out from 15 May to 30 June 2011 investigate diffusion CRE Italy characterise most prevalent resistance mechanisms their dissemination patterns. were (23 25) participating laboratories, with an...
To evaluate the water compartment antibiotic-resistance contamination rates, 11 wells, five streams, and four treatment plants located in Oltrepò Pavese area were screened for presence of third generation cephalosporins resistant Gram-negative bacteria. Enterobacteriaceae also characterized Extended-Spectrum-β-Lactamases (ESBLs), carbapenemases, mcr-1 genes presence. From December 2014 to November 2015, 246 samples filtered, plated on Plate Count Agar, MacConkey Agar with cefotaxime....
The new antitubercular drug candidate 2-[2-S-methyl-1,4-dioxa-8-azaspiro[4.5]dec-8-yl]-8-nitro-6-(trifluoromethyl)-4H-1,3-benzothiazin-4-one (BTZ043) targets the DprE1 (Rv3790) subunit of enzyme decaprenylphosphoryl-beta-d-ribose 2'-epimerase. To monitor potential development benzothiazinone (BTZ) resistance, a total 240 sensitive and multidrug-resistant Mycobacterium tuberculosis clinical isolates from four European hospitals were surveyed for presence mutations in dprE1 gene BTZ...
Abstract Background Despite therapeutic hypothermia (TH) and neonatal intensive care, 45–50% of children affected by moderate-to-severe hypoxic-ischemic encephalopathy (HIE) die or suffer from long-term neurodevelopmental impairment. Additional neuroprotective therapies are sought, besides TH, to further improve the outcome infants. Allopurinol — a xanthine oxidase inhibitor reduced production oxygen radicals subsequent brain damage in pre-clinical preliminary human studies cerebral ischemia...
ABSTRACT Forty-four nonreplicate clinical isolates of Pseudomonas aeruginosa that were resistant to extended-spectrum cephalosporins (ceftazidime and cefepime) aztreonam, putatively produced an acquired extended- spectrum β-lactamase (ESBL), according the results a double-disk synergy test, had been involved in nosocomial outbreaks obtained from six different hospitals northern Italy screened for presence bla PER ESBL determinants. Twenty isolates, associated with nine independent occurred...
Beta-lactamase production was detected in 147 (52%) of 282 consecutive nonduplicate Proteus mirabilis isolates obtained over a 1-year period from the S. Matteo Hospital Pavia (northern Italy). Seventy (48% beta-lactamase producers) were found to produce extended-spectrum beta-lactamases (ESBLs), identified as PER-1 (first report this species) and TEM-52 52 18 isolates, respectively. Analysis clonal diversity ESBL producers suggested different spreading patterns for two determinants.
Abstract Background Acinetobacter baumannii is emerging as an important nosocomial pathogen. Multidrug resistance, well ability to withstand environmental stresses, makes eradication of A. difficult, particularly from hospital settings. Results Over a six-year period, 73 isolates were collected infected patients in two hospitals Italy. While 69 out the displayed identical multidrug antibiotic resistance pattern, they susceptible carbapenems. Genetic profiles these isolates, determined by...
ABSTRACT Ten multidrug-resistant Pseudomonas aeruginosa strains producing VIM-1-like acquired metallo-β-lactamases (MBLs), isolated from four European countries (Greece, Hungary, Italy, and Sweden), were analyzed for genetic relatedness by several methodologies, including fliC sequence analysis, macrorestriction profiling of genomic DNA pulsed-field gel electrophoresis (PFGE), random amplification polymorphic (RAPD), multilocus typing (MLST). The approaches yielded consistent results overall...
Extended-spectrum beta-lactamases (ESBLs), AmpC-type (ACBLs) and carbapenemases are among the most important resistance mechanisms in Enterobacteriaceae . This study investigated presence of these consecutive non-replicate isolates Escherichia coli (n = 2,352), Klebsiella pneumoniae 697), Proteus mirabilis 275) from an Italian nationwide cross-sectional survey carried out October 2013. Overall, 15.3% were non-susceptible to extended-spectrum cephalosporins but susceptible carbapenems...
Carbapenem-resistant Acinetobacter baumannii (CRAb) is emerging worldwide as a public health problem in various settings. The aim of this study was to investigate the prevalence CRAb isolates Italy and characterize their resistance mechanisms genetic relatedness. A countrywide cross-sectional survey carried out at 25 centers mid-2011. were reported from all participating centers, with overall proportions 45.7% 22.2% among consecutive nonreplicate clinical A. inpatients (n = 508) outpatients...
Nanotechnology is a promising approach both for restoring or enhancing activity of old and conventional antimicrobial agents treating intracellular infections by providing targeting sustained release drug inside infected cells. The present paper introduces formulation study gentamicin loaded biodegradable nanoparticles (Nps). Solid-oil-in water technique was studied sulfate nanoencapsulation using uncapped Polylactide-co-glycolide (PLGA-H) Polylactide-co-glycolide-co-Polyethylenglycol...
ObjectiveTo develop a consensus-based set of generic competencies in antimicrobial prescribing and stewardship for European prescribers through structured consensus procedure.MethodsThe RAND-modified Delphi procedure comprised two online questionnaire rounds, face-to-face meeting between final review. Our departure point was agreed previously by among UK multi-disciplinary panel, which had been subsequently revised consultation with ESCMID Study Group representatives. The 46 draft competency...
The COVID-19 pandemic has highlighted the vulnerability of specific population sections, with regards to economic and work conditions, mental physical well-being, context-based factors, emphasizing need for timely policy measures aimed at counteracting Italian framework's fragility-which poorly adapts unexpected circumstances. Identifying most vulnerable groups is, therefore, essential a view carrying out targeted measures. Concerning University, downturn caused by could likely result in...
A microdilution test measuring imipenem MICs in the presence or absence of a mixture EDTA plus 1,10-phenanthroline was developed and tested on 190 Pseudomonas aeruginosa isolates, including 18 VIM- 4 IMP-type metallo-beta-lactamase (MBL) producers. The chelator reduced by fourfold more for MBL producers, while lower effect no usually observed with nonproducers.
ABSTRACT An outbreak of Pseudomonas aeruginosa showing a multidrug-resistant (MDR) phenotype (including carbapenems, ceftazidime, cefepime, gentamicin, tobramycin, and fluoroquinolones) was observed, during 5-month period, in general intensive care unit large tertiary clinical research hospital southern Italy. The involved 15 patients, with total 87 isolates, mostly from lower respiratory tract specimens. Analysis isolates the revealed production metallo-β-lactamase (MBL) activity,...
ABSTRACT We report multifocal detection (four different cities in northern Italy) of Proteus mirabilis isolates resistant to both oxyimino- and 7-α-methoxy-cephalosporins producing a novel acquired AmpC-like β-lactamase. The enzyme, named CMY-16, is variant the CMY/LAT lineage, which differs from closest homologues, CMY-4 CMY-12, by single amino acid substitution (A171S or N363S, respectively) CMY-2 two substitutions W221R). Expression cloned bla CMY-16 gene Escherichia coli decreased...
Abstract Background Rationale and aims of the study were to compare colonization frequencies with MDR bacteria isolated from LTCF residents in three different Northern Italian regions, investigate risk factors for genotypic characteristics isolates. The screening included Enterobacteriaceae expressing extended-spectrum β-lactamases (ESβLs) high-level AmpC cephalosporinases, carbapenemase-producing , Pseudomonas aeruginosa or Acinetobacter baumannii methicillin-resistant Staphylococcus aureus...
Three epidemiologically independent Pseudomonas aeruginosa isolates, representative of the first VIM-1 metallo-beta-lactamase producers detected at three different hospitals in northern Italy, were investigated to determine their genomic relatedness and compare structures genetic supports for determinants. The all serotype O11, appeared be clonally related according results genotyping by macrorestriction analysis DNA pulsed-field gel electrophoresis random amplification polymorphic DNA....
Metallo-beta-lactamases (MBLs) can confer resistance to most beta-lactams, including carbapenems. Their emergence in gram-negative pathogens is a matter of major concern. Italy was the first European country report presence acquired MBLs and one countries where MBL producers have been detected repeatedly. Here, we present results Italian nationwide survey pathogens. Of 14,812 consecutive nonreplicate clinical isolates (12,245 Enterobacteriaceae 2,567 nonfermenters) screened for reduced...