A5053534697- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Enterobacteriaceae and Cronobacter Research
- Urinary Tract Infections Management
- Escherichia coli research studies
- Antibiotic Use and Resistance
- Antibiotics Pharmacokinetics and Efficacy
- Infections and bacterial resistance
- Vibrio bacteria research studies
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Mycobacterium research and diagnosis
- Nephrotoxicity and Medicinal Plants
- Water Treatment and Disinfection
- Microbial Metabolism and Applications
- Bacterial Genetics and Biotechnology
- Complement system in diseases
- Pneumonia and Respiratory Infections
- Veterinary Medicine and Surgery
- Microbial Community Ecology and Physiology
- Bacterial Identification and Susceptibility Testing
- Salmonella and Campylobacter epidemiology
- Antimicrobial Resistance in Staphylococcus
- Veterinary Equine Medical Research
University of Pavia
2017-2025
Charles University
2020-2023
To evaluate the water compartment antibiotic-resistance contamination rates, 11 wells, five streams, and four treatment plants located in Oltrepò Pavese area were screened for presence of third generation cephalosporins resistant Gram-negative bacteria. Enterobacteriaceae also characterized Extended-Spectrum-β-Lactamases (ESBLs), carbapenemases, mcr-1 genes presence. From December 2014 to November 2015, 246 samples filtered, plated on Plate Count Agar, MacConkey Agar with cefotaxime....
The aim of this study was to characterize four Enterobacterales co-producing NDM- and OXA-48-like carbapenemases from Czech patients with travel history or/and previous hospitalization abroad. Klebsiella pneumoniae isolates belonged “high risk” clones ST147, ST11, ST15, while the Escherichia coli isolate assigned ST167. All expressed resistance against most β-lactams, including carbapenems, retaining susceptibility colistin. Furthermore, analysis WGS data showed that all co-produced OXA-48-...
Abstract Enterobacter asburiae , member of the cloacae complex (ECC) group, shows an increasing clinical relevance being responsible for infections like pneumonia, urinary tract and septicemia. The aim present study was investigation genomic features two XDR E. ST229 strains co-carrying bla NDM-1 VIM-1 determinants, collected in October 2021 June 2022, respectively. Two were from rectal swabs as many patients admitted to cardiopulmonary intensive care unit Fondazione I.R.C.C.S. “Policlinico...
The spread of extended-spectrum beta-lactamase (ESBL)- and carbapenemase-producing Escherichia coli clones in humans, animals, the environment is great concern worldwide. In this study, we characterized four E. (CP-Ec) isolated from human samples. Two isolates ST401, rarely associated with carbapenemase and/or ESBL resistance genes, harbored blaKPC-3 blaVIM-1 respectively, were genetically distant each other. One CP-Ec isolate belonging to ST355, typically found poultry environmental sources...
Abstract Background Rationale and aims of the study were to compare colonization frequencies with MDR bacteria isolated from LTCF residents in three different Northern Italian regions, investigate risk factors for genotypic characteristics isolates. The screening included Enterobacteriaceae expressing extended-spectrum β-lactamases (ESβLs) high-level AmpC cephalosporinases, carbapenemase-producing , Pseudomonas aeruginosa or Acinetobacter baumannii methicillin-resistant Staphylococcus aureus...
Infections caused by carbapenemase-producing bacteria have led to the revival of polymyxins as “last-resort” antibiotic. Since 2016, several reports describing presence plasmid-mediated colistin resistance genes, mcr , in different host species and geographic areas were published.
The dissemination of carbapenemase-producing Escherichia coli, although still at low level, should be continuously monitored. OXA-244 is emerging in Europe, mainly E. coli . In Italy, this carbapenemase was reported from an environmental river sample 2019. We report clinical isolates OXA-244-producing ST131 four patients admitted to acute care hospital Pavia, Italy. association difficult-to-detect determinant with a globally circulating high-risk clone, relevance.
Background: The spread of carbapenemase genes, such as blaNDM-1, in Proteus mirabilis poses a public health threat. aim the study was to characterize genome and plasmids sequences an NDM-1-positive strain (IBCRE14), which isolated 2019 from catheterized patient hospitalized Italy. Methods: Whole sequencing (WGS) IBCRE14 performed on extracted genomic DNA using Sequel I platform. Genome assembly "Microbial Assembly". Genomic analysis conducted by uploading contigs ResFinder PlasmidFinder...
Background: the co-production of carbapenemases and mcr-genes represents a worrisome event in treatment Enterobacteriaceae infections. The aim study was to characterize genomic features two clinical Enterobacter cloacae complex (ECC) isolates, co-producing VIM MCR enzymes, Italy. Methods: species identification antibiotic susceptibility profiling were performed using MALDI-TOF broth microdilution methods, respectively. Transferability blaVIM- mcr- type genes verified through conjugation...
We investigated an Italian OXA-181-producing Escherichia coli clinical isolate (ECS1_14) by whole-genome sequencing. The strain coharbored blaCTX-M-15, blaCMY-2, and qnrS1 genes; it belonged to ST410(Achtman)/ST692(Pasteur) phylogroup A. blaOXA-181 gene was harbored on a plasmid highly similar (99% identity) the pOXA181_EC14828 plasmid, recently reported in China.
Aim of this study was to genetically characterize two carbapenemase-producing Escherichia coli strains obtained from a pediatric patient affected by diarrhea, expressing OXA-181 and/or NDM-5 type enzymes. The above microorganisms were collected in the same Desenzano hospital (Northern Italy) where blaNDM-5 gene detected for first time Italy 3 years ago. One strain (5P), belonged sequence ST405/ST477 (according Pasture/Oxford schemes) and serotype O102:H6. It characterized 130562 bp...
Antimicrobial consumption in veterinary medicine has led to the spread of multi drug-resistance clinically important bacteria, with companion animals and their environment involved as emerging reservoirs. While CTX-M-15 CMY-2 acquired β-lactamases have been widely detected bacterial population breeding European area, DHA-1 enzymes rarely reported medicine. The aim study was characterize Escherichia coli associated mortality a litter Bulldog puppies kennel located Pesaro Central Italy. E....
We report two KPC-producing Citrobacter freundii isolates from unrelated patients. In one case, bla KPC-2 was harbored on a novel variant of Tn 4401 transposon an IncN plasmid conjugated together with coresident IncA plasmid, whereas in the other one, KPC-3 4401a located IncX3-IncA self-conjugative fusion.
VIM metallo-β-lactamases are enzymes characterized by the ability to hydrolyze all β-lactams. Usually, bla VIM-like genes carried class 1 integrons. In Czech Republic, only sporadic cases of VIM-producing Enterobacterales have been reported in which those isolates VIM-1 carbapenemase-encoding integron In110. However, during 2019-2020, an increased number was reported. Therefore, aim current study characterize genetic elements involved spread genes. 32 collected between 2019 and 2020 were...
Fosfomycin (FOS) has been recently reintroduced into clinical practice, but its effectiveness against multidrug-resistant (MDR) Enterobacterales is reduced due to the emergence of FOS resistance. The copresence carbapenemases and resistance could drastically limit antibiotic treatment. aims this study were (i) investigate fosfomycin susceptibility profiles among carbapenem-resistant (CRE) in Czech Republic, (ii) characterize genetic environment fosA genes collection, (iii) evaluate presence...
Aim of the study was molecular characterization 21 ceftazidime/avibactam resistant (CZA-R) Klebsiella pneumoniae strains, collected in period October 2021 - March 2022 from an Intensive Care COVID Unit a Northern Italian Hospital. After growth on selective/chromogenic culture media and susceptibility tests assessment, resistance genes content ascertained for all isolates by HybriSpot 12 multiplexing, PCR Whole-Genome Sequencing (WGS). Clonality assessed PFGE MLST according to Pasteur scheme....
Fosfomycin-resistant FosA8-producing
KPC-producing
The study aimed to investigate (i) the occurrence of third-generation cephalosporins and/or carbapenems non-sensitive Enterobacterales in Pavia surface and groundwaters, (ii) their resistance determinants, (iii) clonal features most relevant strains. During May 13 14, 2019, n = 18 water samples from 12 sampling sites urban/peri-urban area (Po Plain, Northern Italy) have been evaluated. At first, hydrochemical analysis bacterial plate counts were carried out on all samples. One milliliter...
Background: VIM (Verona Integron-encoded Metallo-beta-lactamase) is a member of the Metallo-Beta-Lactamases (MBLs), and able to hydrolyze all beta-lactams antibiotics, except for monobactams, including carbapenems. Here we characterize VIM-producing IncA plasmid isolated from clinical ST69 Escherichia coli strain an Italian Long-Term Care Facility (LTCF) inpatient. Methods: An antimicrobial susceptibility test conjugation assay were carried out, transferability blaVIM-type gene was confirmed...