A5029029704- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotics Pharmacokinetics and Efficacy
- Antibiotic Use and Resistance
- Vibrio bacteria research studies
- Escherichia coli research studies
- Urinary Tract Infections Management
- Mycobacterium research and diagnosis
- Infections and bacterial resistance
- Enterobacteriaceae and Cronobacter Research
- Bacteriophages and microbial interactions
- Tuberculosis Research and Epidemiology
- Genomics and Phylogenetic Studies
- Bacterial Identification and Susceptibility Testing
- Medical Device Sterilization and Disinfection
- Salmonella and Campylobacter epidemiology
- Plant Pathogenic Bacteria Studies
- Pneumonia and Respiratory Infections
- Respiratory viral infections research
- Microbial Metabolism and Applications
- Diverse academic and cultural studies
- Antimicrobial Resistance in Staphylococcus
- Microbial Community Ecology and Physiology
- Essential Oils and Antimicrobial Activity
University of Pavia
2016-2025
Policlinico San Matteo Fondazione
2024-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2005-2025
United States Nuclear Regulatory Commission
2012
University of Siena
2007
Twelve isolates of Enterobacteriaceae (1 Klebsiella pneumoniae, 8 Escherichia coli, 1 Proteus mirabilis, and 2 vulgaris) classified as extended-spectrum beta-lactamase (ESBL) producers according to the ESBL screen flow application BD-Phoenix automatic system for which cefotaxime MICs were higher than those ceftazidime collected between January 2001 July 2002 at Laboratory Clinical Microbiology San Matteo University Hospital Pavia (northern Italy). By PCR sequencing, a CTX-M-type determinant...
Carbapenem-resistant Enterobacteriaceae (CRE) are emerging as a public health problem in various settings. In Italy, rapid and remarkable increase of carbapenem-non-susceptible Klebsiella pneumoniae has been reported since 2010. Here we report on the results countrywide cross-sectional survey, carried out from 15 May to 30 June 2011 investigate diffusion CRE Italy characterise most prevalent resistance mechanisms their dissemination patterns. were (23 25) participating laboratories, with an...
To evaluate the water compartment antibiotic-resistance contamination rates, 11 wells, five streams, and four treatment plants located in Oltrepò Pavese area were screened for presence of third generation cephalosporins resistant Gram-negative bacteria. Enterobacteriaceae also characterized Extended-Spectrum-β-Lactamases (ESBLs), carbapenemases, mcr-1 genes presence. From December 2014 to November 2015, 246 samples filtered, plated on Plate Count Agar, MacConkey Agar with cefotaxime....
ABSTRACT Forty-four nonreplicate clinical isolates of Pseudomonas aeruginosa that were resistant to extended-spectrum cephalosporins (ceftazidime and cefepime) aztreonam, putatively produced an acquired extended- spectrum β-lactamase (ESBL), according the results a double-disk synergy test, had been involved in nosocomial outbreaks obtained from six different hospitals northern Italy screened for presence bla PER ESBL determinants. Twenty isolates, associated with nine independent occurred...
Beta-lactamase production was detected in 147 (52%) of 282 consecutive nonduplicate Proteus mirabilis isolates obtained over a 1-year period from the S. Matteo Hospital Pavia (northern Italy). Seventy (48% beta-lactamase producers) were found to produce extended-spectrum beta-lactamases (ESBLs), identified as PER-1 (first report this species) and TEM-52 52 18 isolates, respectively. Analysis clonal diversity ESBL producers suggested different spreading patterns for two determinants.
Sequential nasopharyngeal aspirates from patients without respiratory symptoms undergoing hematopoietic stem cell transplantation (HSCT) were tested for genomic RNA of human metapneumovirus (hMPV). Persistent hMPV infection was documented in most them and confirmed by virus isolation. etiology also evaluated during the same period samples pediatric with acute diseases (ARDs). Sequence analysis HSCT recipients genotype A strong interhost similarity; this pattern differs that observed ARDs....
Abstract Background Acinetobacter baumannii is emerging as an important nosocomial pathogen. Multidrug resistance, well ability to withstand environmental stresses, makes eradication of A. difficult, particularly from hospital settings. Results Over a six-year period, 73 isolates were collected infected patients in two hospitals Italy. While 69 out the displayed identical multidrug antibiotic resistance pattern, they susceptible carbapenems. Genetic profiles these isolates, determined by...
Nanotechnology is a promising approach both for restoring or enhancing activity of old and conventional antimicrobial agents treating intracellular infections by providing targeting sustained release drug inside infected cells. The present paper introduces formulation study gentamicin loaded biodegradable nanoparticles (Nps). Solid-oil-in water technique was studied sulfate nanoencapsulation using uncapped Polylactide-co-glycolide (PLGA-H) Polylactide-co-glycolide-co-Polyethylenglycol...
The dissemination of carbapenemase-producing Escherichia coli, although still at low level, should be continuously monitored. OXA-244 is emerging in Europe, mainly E. coli . In Italy, this carbapenemase was reported from an environmental river sample 2019. We report clinical isolates OXA-244-producing ST131 four patients admitted to acute care hospital Pavia, Italy. association difficult-to-detect determinant with a globally circulating high-risk clone, relevance.
Abstract The global race against antimicrobial resistance requires novel antimicrobials that are not only effective in killing specific bacteria, but also minimize the emergence of new resistances. Recently, CRISPR/Cas-based were proposed to address specificity with encouraging results. However, target sequence mutations triggered by Cas-cleavage was identified as an escape strategy, posing risk generating antibiotic-resistance gene (ARG) variants. Here, we evaluated antibiotic...
The spread of extended-spectrum beta-lactamase (ESBL)- and carbapenemase-producing Escherichia coli clones in humans, animals, the environment is great concern worldwide. In this study, we characterized four E. (CP-Ec) isolated from human samples. Two isolates ST401, rarely associated with carbapenemase and/or ESBL resistance genes, harbored blaKPC-3 blaVIM-1 respectively, were genetically distant each other. One CP-Ec isolate belonging to ST355, typically found poultry environmental sources...
A microdilution test measuring imipenem MICs in the presence or absence of a mixture EDTA plus 1,10-phenanthroline was developed and tested on 190 Pseudomonas aeruginosa isolates, including 18 VIM- 4 IMP-type metallo-beta-lactamase (MBL) producers. The chelator reduced by fourfold more for MBL producers, while lower effect no usually observed with nonproducers.
ABSTRACT An outbreak of Pseudomonas aeruginosa showing a multidrug-resistant (MDR) phenotype (including carbapenems, ceftazidime, cefepime, gentamicin, tobramycin, and fluoroquinolones) was observed, during 5-month period, in general intensive care unit large tertiary clinical research hospital southern Italy. The involved 15 patients, with total 87 isolates, mostly from lower respiratory tract specimens. Analysis isolates the revealed production metallo-β-lactamase (MBL) activity,...
ABSTRACT We report multifocal detection (four different cities in northern Italy) of Proteus mirabilis isolates resistant to both oxyimino- and 7-α-methoxy-cephalosporins producing a novel acquired AmpC-like β-lactamase. The enzyme, named CMY-16, is variant the CMY/LAT lineage, which differs from closest homologues, CMY-4 CMY-12, by single amino acid substitution (A171S or N363S, respectively) CMY-2 two substitutions W221R). Expression cloned bla CMY-16 gene Escherichia coli decreased...
Abstract Background Rationale and aims of the study were to compare colonization frequencies with MDR bacteria isolated from LTCF residents in three different Northern Italian regions, investigate risk factors for genotypic characteristics isolates. The screening included Enterobacteriaceae expressing extended-spectrum β-lactamases (ESβLs) high-level AmpC cephalosporinases, carbapenemase-producing , Pseudomonas aeruginosa or Acinetobacter baumannii methicillin-resistant Staphylococcus aureus...
Bacteriophages are the most abundant organisms on Earth. As there few effective treatment options against some pathogens, interest in bacteriophage control of multi-drug-resistant bacterial pathogens is escalating, especially for Klebsiella pneumoniae. This study aimed to develop a phage-based solution rising incidence extensively drug-resistant clinical pneumoniae sequence type (ST16) infections starting from set phages recently characterized this lineage. A phage-cocktail (Katrice-16)...
Metallo-beta-lactamases (MBLs) can confer resistance to most beta-lactams, including carbapenems. Their emergence in gram-negative pathogens is a matter of major concern. Italy was the first European country report presence acquired MBLs and one countries where MBL producers have been detected repeatedly. Here, we present results Italian nationwide survey pathogens. Of 14,812 consecutive nonreplicate clinical isolates (12,245 Enterobacteriaceae 2,567 nonfermenters) screened for reduced...
Long Term Care Facilities (LTCFs) play a key role in guaranteeing care to patients developed countries. Many patients, mostly elderly, access LTCFs at some time their lives, and healthcare pathways often require them move back forth between hospital outpatient settings. These patterns bring about new challenges regarding infection control, especially associated infections.A point prevalence study was conducted 23 Italian LTCFs, identify colonization with urinary catheter (>24 hours). Species...
Clinical isolates of Acinetobacter baumannii (n = 470) were collected during a 7-year period and investigated for the genetic determinants resistance to expanded-spectrum beta-lactams. Thirty-one produced TEM-92 extended-spectrum beta-lactamase (ESBL) clonally related. This is first report A. producing TEM-type ESBL.
Metallo-β-lactamases (MBLs) are among the most challenging bacterial enzymes to overcome. Aztreonam (ATM) is only β-lactam not hydrolyzed by MBLs but often inactivated co-produced extended-spectrum β-lactamases (ESBL). We assessed activity of combination ATM with old and new inhibitors (BLIs) against MBL ESBL co-producing Gram-negative clinical isolates. Six Enterobacterales three non-fermenting bacilli determinants were selected as difficult-to-treat pathogens. ESBLs genes characterized PCR...