A5034489186- Plant-Microbe Interactions and Immunity
- Fungal and yeast genetics research
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Plant and fungal interactions
- Plant Pathogenic Bacteria Studies
- Fungal Plant Pathogen Control
- Botanical Research and Chemistry
- Heat shock proteins research
- Endoplasmic Reticulum Stress and Disease
- Toxin Mechanisms and Immunotoxins
- Plant Pathogens and Resistance
- Mycotoxins in Agriculture and Food
- Plant Toxicity and Pharmacological Properties
- Mycorrhizal Fungi and Plant Interactions
- Fermentation and Sensory Analysis
- Photosynthetic Processes and Mechanisms
- Wheat and Barley Genetics and Pathology
- Plant biochemistry and biosynthesis
Noble Research Institute
2015-2020
Kansas State University
2013-2016
Seoul National University
2007-2009
To cause plant diseases, pathogenic micro-organisms secrete effector proteins into host tissue to suppress immunity and support pathogen growth. Bacterial pathogens have evolved several distinct secretion systems target proteins, but whether fungi, which the major diseases of most crop species, also require different secretory mechanisms is not known. Here we report that rice blast fungus Magnaporthe oryzae possesses two effectors during infection. Cytoplasmic effectors, are delivered cells,...
In planta secretion of fungal pathogen proteins, including effectors destined for the plant cell cytoplasm, is critical disease progression. However, little known about endoplasmic reticulum (ER) mechanisms used by these pathogens. To determine if normal ER function crucial pathogenicity, Magnaporthe oryzae genes encoding proteins homologous to yeast Lhs1p and Kar2p, members heat shock protein 70 family in Saccharomyces cerevisiae, were cloned characterized. Like their counterparts, both...
Summary Phytopathogenic microorganisms, including the fungal pathogen Magnaporthe oryzae , secrete a myriad of effector proteins to facilitate infection. Utilizing transient expression candidate effectors in leaves model plant Nicotiana benthamiana we identified 11 suppressors cell death (SPD) from M. that were able block host reaction induced by Nep1. Ten these also suppress BAX‐mediated death. Five SPD genes have been previously as either essential for pathogenicity secreted into during...
As part of a large-scale project whose goal was to identify candidate effector proteins in Magnaporthe oryzae, we developed suite vectors that facilitate high-throughput protein localization experiments fungi. These utilize Gateway recombinational cloning place gene's promoter and coding sequences upstream frame with enhanced cyan fluorescent protein, green (GFP), monomeric red fluorescence (mRFP), yellow or nucleus-targeted mCHERRY variant. The respective cassettes were incorporated into...
Epichloë species (including the former genus Neotyphodium) are fungal symbionts of many agronomically important forage grasses, and provide their grass hosts with protection from a wide range biotic abiotic stresses. include interspecific hybrids allodiploid-like genomes, which may potential for combined traits or recombination to generate new traits. Though circumstantial evidence suggests that such might have arisen nuclear fusion events following vegetative hyphal between different...
Pecan scab, caused by Venturia effusa, is the most prevalent disease of pecan in southeastern United States. Recent characterization mating type (MAT) distribution V. effusa revealed that MAT idiomorphs are equilibrium at various spatial scales, indicative regular sexual recombination. However, occurrence stage has never been observed, and pathogen was previously considered to rely entirely on asexual reproduction. We were able generate pairing isolates opposite types oatmeal culture media....
Epichloë species (Clavicipitaceae, Ascomycota) are endophytic symbionts of many cool-season grasses. Many interactions between and their host grasses contribute to plant growth promotion, protection from pathogens insect pests, tolerance drought stress. Resistance herbivores by endophytes associated with Hordeum has been previously shown vary depending on the endophyte-grass-insect combination. We explored genetic chemotypic diversity present in wild species. analyzed seeds bogdanii, H....
Magnaporthe oryzae, the causal agent of rice blast disease, poses a worldwide threat to stable production. The large-scale functional characterization genes controlling pathogenicity M. oryzae is currently under way, but little known about heat shock protein 40 (Hsp40) function in fungus or any other filamentous plant pathogen. We identified 25 encoding putative Hsp40s genome using bioinformatic approach, which we designated forty (MHF 1-25). To elucidate roles these genes, characterized...
ABSTRACT Venturia effusa , cause of pecan scab, is the most prevalent disease in southeastern USA; epidemics regularly result economic losses to industry. Recent characterization mating type distribution revealed frequency MAT idiomorphs are equilibrium at various spatial scales, indicative regular sexual recombination. However, occurrence stage V. has never been observed, and pathogen was previously believed rely entirely on asexual reproduction. To explore existence a cycle, we paired...