A5026991667- Porphyrin and Phthalocyanine Chemistry
- Photochemistry and Electron Transfer Studies
- Lipid Membrane Structure and Behavior
- Advanced biosensing and bioanalysis techniques
- Organic Electronics and Photovoltaics
- Mosquito-borne diseases and control
- Spectroscopy and Quantum Chemical Studies
- Plant Molecular Biology Research
- Insect symbiosis and bacterial influences
- Polysaccharides and Plant Cell Walls
- Supramolecular Self-Assembly in Materials
- Graphene research and applications
- Invertebrate Immune Response Mechanisms
- RNA modifications and cancer
- Crystallization and Solubility Studies
- Indoor and Outdoor Localization Technologies
- Conducting polymers and applications
- X-ray Diffraction in Crystallography
- Speech and Audio Processing
- Gold and Silver Nanoparticles Synthesis and Applications
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- Advanced Fluorescence Microscopy Techniques
- Luminescence and Fluorescent Materials
- Plant and Biological Electrophysiology Studies
John Innes Centre
2022-2025
Boston Children's Hospital
2025
Boston University
2025
Harvard University
2025
Norwich Research Park
2022-2025
University of Massachusetts Boston
2022-2024
Institute of Nanostructured Materials
2017-2021
Institute of Structure of Matter
2017-2019
Buenos Aires Institute of Technology
2018
University of Glasgow
2018
Abstract O -linked β- N -acetylglucosamine ( -GlcNAc) and -fucose are two sugar-based post-translational modifications whose mechanistic role in plant signalling transcriptional regulation is still largely unknown. Here we investigated how -glycosyltransferase enzymes of Arabidopsis thaliana , SPINDLY (SPY) SECRET AGENT (SEC), promote the activity basic helix–loop–helix transcription factor SPATULA (SPT) during morphogenesis female reproductive organ apex, style. SPY SEC modify...
The rice blast fungus Magnaporthe oryzae uses a pressurized infection cell called an appressorium to drive rigid penetration peg through the leaf cuticle. vast internal pressure of is very challenging investigate, leaving our understanding cellular mechanics plant incomplete. Here, using fluorescence lifetime imaging membrane-targeting molecular mechanoprobe, we quantify changes in membrane tension M. oryzae. We show that extreme leads large-scale spatial heterogeneities mechanics, much...
Abstract Colocalisation microscopy analysis provides an intuitive and straightforward way of determining if two biomolecules occupy the same diffraction‐limited volume. A popular colocalisation coefficient, Pearson's correlation coefficient (PCC), can be calculated using different pixel selection criteria: PCC ALL includes all image pixels, OR only pixels exceeding intensity thresholds for either one detection channels, AND both channels. Our results show that depends on foreground to...
Ag nanopatterned arrays prepared using periodically proton exchanged templates have been demonstrated to support surface enhanced luminescence. Fluorescence lifetime imaging reveals that luminescence intensity is greatest on and the of molecular probe reduced, in line with a mechanism. Studies establish substrate simultaneously supports Raman scattering. Spatial dependence along shows <7% variation scattering signal intensity, offering high reproducibility for practical applications....
We examine here a series of meso-phenyl porphyrin micro- and nanostructures. Optical absorption emission spectroscopy imaging atomic force microscopy are used to investigate the effect peripheral groups in nano- microstructures 5,10,15,20-tetraphenylporphyrin (H2TPP) compared three other phenylporphyrins, i.e. 5,10,15-triphenylporphyrin (H2-Tri-PP), 5,10-diphenylporphyrin (H25,10-BPP) 5,15-diphenylporphyrin (H25,15-BPP) molecules. show that nanospheres nanorods formed, occurrence properties...
From gene expression to nanotechnology, understanding and controlling DNA requires a detailed knowledge of its higher order structure dynamics. Here we take advantage the environment-sensitive photoisomerization cyanine dyes probe local global changes in structure. We report that covalently attached Cy3 dye undergoes strong enhancement fluorescence intensity lifetime when stacked nick, gap or overhang region duplex DNA. This is used hybridization dynamics hairpin down single-molecule level....
A novel method for reconstitution of Integrin α<sub>IIb</sub>β<sub>3</sub>into GUVs with unrestricted lipid composition is described and the lateral diffusion phase partitioning integrin on activation ligand binding in biomimetic compositions studied formulations.
The impact of the processing method in controlling polymorphism and field-effect charge mobility 2,3-thienoimide-based oligothiophenes semiconductors was investigated.
Mosquitoes are a complex nuisance around the world and tropical countries bear brunt of burden mosquito-borne diseases. Rwanda has had success in reducing malaria some arboviral diseases over last few years, but still faces challenges to elimination. By building our understanding situ mosquito communities at disturbed, human-occupied site natural, preserved site, we can build natural microbiomes toward goal implementing novel microbial control methods. Here, examined composition collected...
Rhodamine 6G (R6G) was adsorbed on cellulose microparticles and fluorescence quantum yields decays were measured as a function of dye loading. Though no spectroscopic evidence aggregation found, noticeable decrease yield--after correction for reabsorption reemission fluorescence--and shortening observed at the highest loadings. These effects attributed to dissipation excitation energy by traps constituted R6G pairs, leading static dynamic quenching produced direct absorption non-radiative...
Ficoll has been widely used as a crowding agent to mimic intracellular media because it is believed be noninteracting and composed of mixed sizes such that smaller larger diffusing solutes can studied. Due the interest fluorescent dye 9-[1-(2-methyl-4-methoxyphenyl)]-6-hydroxy-3H-xanthen-3-one (TG-II) fluorometric probe phosphate ions in could generate, we describe spectral characteristics system TG-II-Ficoll aqueous solution by means absorption spectroscopy, steady-state fluorescence,...
Studies are undertaken to examine graphene oxide intercalation into self-assembled J-aggregate porphyrin structures. Fluorescence lifetime and fluorescence anisotropy imaging were applied along with scanning electron microscopy study the structure optical properties of a oxide/TMPyP hybrid composite material. It was seen that presence alters macroscale nanoscale structures TMPyP in addition also activity reducing emission intensity exciton recombination lifetime. Evidence exists support...
The production of designer-length tobacco mosaic virus (TMV) nanorods in plants has been problematic terms yields, particularly when modified coat protein subunits are incorporated. To address this, we have investigated the use a replicating potato X-based vector (pEff) to express defined length containing either wild-type or versions TMV protein. This system previously shown be an efficient method for producing virus-like particles filamentous plant viruses. resulting can controlled by...
Abstract Our understanding of the functional requirements underpinning biomolecular condensation in vivo is still relatively poor. The Arabidopsis RNA binding protein FCA found liquid-like nuclear condensates that function transcription termination, promoting proximal polyadenylation at many targets genome. To further understand properties these we used single particle tracking experiments on reporters stably expressed endogenous levels plant nuclei. These revealed forms a core oligomer ∼4...
The proton-transfer ground-state rate constants of the xanthenic dye 9-[1-(2-methyl-4-methoxyphenyl)]-6-hydroxy-3H-xanthen-3-one (TG-II), recovered by Fluorescence Lifetime Correlation Spectroscopy (FLCS), have proven to be useful quantitatively reflect specific cation effects in aqueous solutions (J. M. Paredes, L. Crovetto, A. Orte, J. Alvarez-Pez and E. Talavera, Phys. Chem. Phys., 2011, 13, 1685–1694). Since these phenomena are more sensitive anions than cations, this paper we accounted...
In this work we aim to (via a non-invasive functionalization approach) tune and alter the intrinsic features of optically "transparent" graphene, by integrating water-soluble porphyrin aggregates. We explore potential combine aggregates graphene oxide assess advantages such as composite compared individual systems. apply range optical spectroscopy methods including photo-absorption, fluorescence ground-state excited state interactions. Our studies show that comparing resonant Raman...
The inorganic/molecular spinterface is an ideal platform for generating extraordinary spin effects. Understanding and controlling these spin-related effects mandatory the exploitation of such interfaces in devices. For this purpose we have investigated adsorption α-sexithiophene (α-6T) on La0.7Sr0.3MnO3 (LSMO) as one prototypical material combinations used organic spintronic Atomic force microscopy (AFM), confocal photoluminescence, X-ray utraviolet photoelectron spectroscopy, metastable...
ABSTRACT The establishment of organ symmetry during multicellular development is a fundamental process shared by most living organisms. Here, we investigated how two O -glycosyltransferases Arabidopsis thaliana , SPINDLY (SPY) and SECRET AGENT (SEC) synergistically promote rare bilateral-to-radial transition patterning the plant reproductive organ, gynoecium. SPY SEC modify N-terminal residues bHLH transcription factor SPATULA (SPT) in vivo vitro attaching -fucose...
There is abundant literature on positioning systems based WiFi signals. Most of the with off-the-shelf hardware use RSSI measurements. In this paper, we try to determine highest achievable accuracy such systems. Instead resorting a theoretical analysis, e.g., Cramér-Rao or Ziv-Zakai bounds, apply state-of-the-art localization algorithms measurements in well-controlled experimental setup. We conclude that an less than 1 meter seems unrealistic.
The crystallization of 2,2′-(5,5′-(ethyne-1,2-diyl)bis(thiophene-5,2-diyl))bis(5-hexyl-4H-thieno[2,3-c]pyrrole-4,6(5H)-dione), hereafter NTA, in different solvents and/or conditions yielded the identification five polymorphs which are characterized by impressive differences their photophysical behavior. Phase I, phase II, III, and IV a yellow, green, red, orange emission under illumination with 365 nm light. crystal structures II III were determined single-crystal X-ray diffraction. is...