A5006530180- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Horticultural and Viticultural Research
- Plant Physiology and Cultivation Studies
- Aluminum toxicity and tolerance in plants and animals
- Plant Micronutrient Interactions and Effects
- Membrane-based Ion Separation Techniques
- Ion channel regulation and function
- Plant responses to water stress
- Ion Transport and Channel Regulation
- Magnetic and Electromagnetic Effects
- Postharvest Quality and Shelf Life Management
- Fermentation and Sensory Analysis
- Plant Taxonomy and Phylogenetics
- Microfluidic and Bio-sensing Technologies
- Genetics, Aging, and Longevity in Model Organisms
- Mediterranean and Iberian flora and fauna
- Soybean genetics and cultivation
- Legume Nitrogen Fixing Symbiosis
- Neonatal Health and Biochemistry
- Plant tissue culture and regeneration
- Industrial Vision Systems and Defect Detection
- Spaceflight effects on biology
- Botany and Plant Ecology Studies
The University of Adelaide
2013-2024
ARC Centre of Excellence in Plant Energy Biology
2015-2024
Australian Wine Research Institute
2024
Australian Research Council
2013-2022
Agriculture and Food
2018-2020
Commonwealth Scientific and Industrial Research Organisation
2018-2020
Australian Centre for Plant Functional Genomics
2015
University of Colorado Boulder
2001
Abstract Background Hydroponic growth systems are a convenient platform for studying whole plant physiology. However, we found through trialling as they described in the literature that our experiments were frequently confounded by factors affected growth, including algal contamination and hypoxia. We also way which plants grown made them poorly amenable to number of common physiological assays. Results The drivers development this hydroponic system were: 1) exclusion light from solution; 2)...
The aquaporin AtPIP2;1 is an abundant plasma membrane intrinsic protein in Arabidopsis thaliana that implicated stomatal closure, and highly expressed membranes of root epidermal cells. When Xenopus laevis oocytes, increased water permeability induced a non-selective cation conductance mainly associated with Na+ . A mutation the pore, G103W, prevented both ionic PIP2;1. Co-expression AtPIP1;2 but abolished conductance. AtPIP2;2 (93% identical to AtPIP2;1) similarly not was inhibited by...
Salt tolerance in grapevine is associated with chloride (Cl-) exclusion from shoots; the rate-limiting step being passage of Cl- between root symplast and xylem apoplast. Despite an understanding physiological mechanism grapevine, molecular identity membrane proteins that control this process have remained elusive. To elucidate candidate genes likely to exclusion, we compared transcriptomes three Vitis spp. contrasting shoot capacities using a custom microarray.When challenged 50 mM Cl-,...
Under saline conditions, higher plants restrict the accumulation of chloride ions (Cl–) in shoot by regulating their transfer from root symplast into xylem-associated apoplast. To identify molecular mechanisms underpinning this phenomenon, we undertook a transcriptional screen salt stressed Arabidopsis (Arabidopsis thaliana) roots. Microarrays, quantitative RT-PCR, and promoter-GUS fusions identified candidate gene involved Cl– xylem loading Nitrate transporter 1/Peptide Transporter family...
Salinity tolerance is correlated with shoot chloride (Cl(-)) exclusion in multiple crops, but the molecular mechanisms of long-distance Cl(-) transport are poorly defined. Here, we characterize planta role AtSLAH1 (a homologue slow type anion channel-associated 1 (SLAC1)). This protein, localized to plasma membrane root stelar cells, has its expression reduced by salt or ABA, which key predictions for a protein involved loading into xylem. Artificial microRNA knockdown mutants had...
Under salinity, Vitis spp. rootstocks can mediate salt (NaCl) exclusion from grafted V. vinifera scions enabling higher grapevine yields and production of superior wines with lower content. Until now, the genetic mechanistic elements controlling sodium (Na+ ) in were unknown. Using a cross between two interspecific hybrid rootstocks, we mapped dominant quantitative trait locus (QTL) associated leaf Na+ (NaE) under salinity stress. The NaE encodes six high-affinity potassium transporters...
The global need for increased food production means that agriculture is moving into regions with lower rainfall and saline soils, which occupy over 6% of the world land area (Munns, 2005). aim this workshop was to work towards completing an energy budget mechanisms salinity tolerance in crop plants, as a guide most cost-effective breeding strategies increasing salt yield important crops. discussions took account supply from mitochondria chloroplasts, demands transport water, Na+, Cl− K+,...
Salinity, which is predominantly an issue for agricultural systems in arid and semi-arid regions, has the potential to impair grape production wine quality, its impact on industries predicted increase with climate change. Research physiological molecular changes that occur salt-affected vines unveiled complex osmotic ionic responses include oxidative stress, water loss, photoinhibition, growth inhibition necrosis. Proposed salt tolerance mechanisms elevated antioxidant production, hydric...
Plant cation-chloride cotransporters (CCCs) have been implicated in conferring salt tolerance. They are predicted to improve shoot exclusion by directly catalyzing the retrieval of sodium (Na+) and chloride (Cl−) ions from root xylem. We investigated whether grapevine (Vitis vinifera [Vvi]) CCC has a role tolerance cloning functionally characterizing gene cultivar Cabernet Sauvignon. Amino acid sequence analysis revealed that VviCCC shares high degree similarity with other plant CCCs. A...
Soybean (Glycine max) yields are threatened by multiple stresses including soil salinity. GmSALT3 (a cation-proton exchanger protein) confers net shoot exclusion for both Na+ and Cl- improves salt tolerance of soybean; however, how the ER-localized achieves this is unknown. Here, GmSALT3's function was investigated in heterologous systems near isogenic lines that contained full-length (NIL-T; salt-tolerant) or a truncated transcript Gmsalt3 (NIL-S; salt-sensitive). restored growth K+...
Background and Aims Rooted leaves were used to analyse the salt exclusion phenotype in grapevines. Genotypes included rootstocks 140 Ruggeri K51-40 cultivar Cabernet Sauvignon – respectively, good, poor intermediate chloride excluders. Methods Results We investigated effect of short-term treatment on chloride, sodium potassium concentration organs whole rooted time course accumulation salt-treated, leaves. The Control plus low high nitrate was assessed. Salt increased all but had no...
Most Hieracium subgenus Pilosella species are self-incompatible. Some undergo facultative apomixis where most seeds form asexually with a maternal genotype. embryo sacs develop by mitosis, without meiosis and fertilization. Apomixis is controlled dominant loci recombination suppressed. Loci deletion γ-irradiation results in reversion to sexual reproduction. Targeted mutagenesis of genes at identified would facilitate causal gene identification. In this study, the efficacy CRISPR/Cas9 editing...
In sickle cell disease (SCD), the pathological shift of red blood cells (RBCs) into distorted morphologies under hypoxic conditions follows activation a cationic leak current (Psickle) and dehydration. Prior work showed sickling was reduced by 5-hydroxylmethyl-2-furfural (5-HMF), which stabilized mutant hemoglobin also blocked Psickle in RBCs, though molecular basis this 5-HMF-sensitive cation remained mystery. Work here is first to test hypothesis that Aquaporin-1 (AQP1) channels contribute...
Human aquaporin 1 (hAQP1) forms homotetrameric channels that facilitate fluxes of water and small solutes across cell membranes. In addition to channel activity, hAQP1 displays non-selective monovalent cation-channel activity gated by intracellular cyclic GMP. Dual ion-channel hAQP1, thought regulate shape volume, could offer a target for novel therapeutics relevant controlling cancer invasiveness. This study probed properties ion using proteoliposomes, which, unlike conventional cell-based...
Grapevines ( Vitis vinifera L., Vvi ) on their roots are generally sensitive to salt-forming ions, particularly chloride (Cl – when grown in saline environments. Grafting V. scions Cl -excluding hybrid rootstocks reduces the impact of salinity. Molecular components underlying -exclusion species remain largely unknown, however, various anion channels and transporters represent good candidates for controlling this trait. Here, two nitrate/peptide transporter family (NPF) members VviNPF2.1...
Plant cation-chloride-cotransporters (CCC) are proposed to be Na+–K+–2Cl–transporting proteins, although phylogenetically they closer K+-Cl–cotransporters (KCC). Conserved features of plant CCC and animal KCC include the presence predicted K+ Cl– binding sites, absence a Na+ site. Here, we investigated grapevine (Vitis vinifera L.) VvCCC using protein structural modelling heterologous expression. Our data that 3D folds were more similar DrNKCC1, but sequences ion sites resembled those hKCC1....
Abstract Aquaporin (AQP) channels found in all domains of life are transmembrane proteins which mediate passive transport water, glycerol, signaling molecules, metabolites, and charged solutes. Discovery new classes ion-conducting AQP has been slow, likely reflecting time- labor-intensive methods required for traditional electrophysiology. Work here defines a sensitive mass-throughput system detecting ion channels, identified by rescue cell growth the K+-transport-defective yeast strain...