A5050665345- Plant Stress Responses and Tolerance
- Plant Micronutrient Interactions and Effects
- Plant nutrient uptake and metabolism
- Wheat and Barley Genetics and Pathology
- Plant Molecular Biology Research
- Genetic Mapping and Diversity in Plants and Animals
- Genetics and Plant Breeding
- Photosynthetic Processes and Mechanisms
- Crop Yield and Soil Fertility
- Magnetic and Electromagnetic Effects
- ATP Synthase and ATPases Research
- Plant Genetic and Mutation Studies
- Leaf Properties and Growth Measurement
- Silicon Effects in Agriculture
- Plant and Biological Electrophysiology Studies
- Plant-Microbe Interactions and Immunity
- Plant responses to water stress
- Plant responses to elevated CO2
- Plant Disease Resistance and Genetics
- Remote Sensing in Agriculture
- African Studies and Ethnography
- Aluminum toxicity and tolerance in plants and animals
- Legume Nitrogen Fixing Symbiosis
- Plant Water Relations and Carbon Dynamics
- Agronomic Practices and Intercropping Systems
The University of Adelaide
2015-2024
Kerala Agricultural University
2022-2023
Australian Centre for Plant Functional Genomics
2012-2022
Australian Wine Research Institute
2010-2022
University of South Australia
2022
Australian Research Council
2019-2022
ARC Centre of Excellence in Plant Energy Biology
2015
Purdue University West Lafayette
2013
University of Cambridge
2003-2008
Soil salinity affects large areas of cultivated land, causing significant reductions in crop yield globally. The Na+ toxicity many plants is correlated with overaccumulation the shoot. We have previously suggested that engineering exclusion from shoot could be achieved through an alteration plasma membrane transport processes root, if these alterations were cell type specific. Here, it shown expression transporter HKT1;1 mature root stele Arabidopsis thaliana decreases accumulation by 37 to...
ABSTRACT Salinity stress is a major factor inhibiting cereal yield throughout the world. Tolerance to salinity can be considered contain three main components: Na + exclusion, tolerance in tissues and osmotic tolerance. To date, most experimental work on cereals has focused exclusion due part its ease of measurement. It become apparent, however, that not sole mechanism for cereals, research needs expand study tissue Here, we develop assays high throughput quantification 12 Triticum...
With the establishment of advanced technology facilities for high throughput plant phenotyping, problem estimating biomass individual plants from their two dimensional images is becoming increasingly important. The approach predominantly cited in literature to estimate a as linear function projected shoot area images. However, estimation error this model, which solely area, large, prohibiting accurate plants, particularly salt-stressed plants. In paper, we propose method based on specific...
Plant breeding and improvements in agronomic practice are making a consistent contribution to increasing global crop production year upon year. However, the rate of yield improvement currently lags behind targets set produce enough food meet demands predicted population 2050. Furthermore, crops that exposed harmful abiotic environmental factors (abiotic stresses, e.g. water limitation, salinity, extreme temperature) prone reduced yields. Here, we briefly describe processes undertaken...
Abstract Background Soil salinity is an abiotic stress wide spread in rice producing areas, limiting both plant growth and yield. The development of salt-tolerant requires efficient high-throughput screening techniques to identify promising lines for salt affected areas. Advances made image-based phenotyping provide opportunity use non-destructive imaging screen tolerance traits a range germplasm reliable, quantitative way. However, the application remains limited. Results A protocol assess...
Salinity tolerance is a physiologically multi-faceted trait attributed to multiple mechanisms. Three barley (Hordeum vulgare) varieties contrasting in their salinity were used assess the relative contribution of ionic, osmotic and oxidative stress components towards overall this species, both at whole-plant cellular levels. In addition, transcriptional changes gene expression profile studied for key genes mediating plant ionic homeostasis (NHX; RBOH; SOD; AHA GORK), compare...
Previously, cell type-specific expression of AtHKT1;1, a sodium transporter, improved (Na+) exclusion and salinity tolerance in Arabidopsis. In the current work, was expressed specifically root cortical epidermal cells an Arabidopsis GAL4-GFP enhancer trap line. These transgenic plants were found to have significantly Na+ under conditions stress. The feasibility similar biotechnological approach crop explored using rice line drive AtHKT1;1 cortex. Compared with background line, expressing...
Summary Cereal varieties with improved salinity tolerance are needed to achieve profitable grain yields in saline soils. The expression of AVP 1 , an A rabidopsis gene encoding a vacuolar proton pumping pyrophosphatase ( H + ‐ PP ase), has been shown improve the transgenic plants greenhouse conditions. However, potential for this yield cereal crops field yet be evaluated. Recent advances high‐throughput nondestructive phenotyping technologies also offer opportunity quantitatively evaluate...
ABSTRACT Salinity tolerance can be attributed to three different mechanisms: Na + exclusion from the shoot, tissue and osmotic tolerance. Although several key ion channels transporters involved in these processes are known, variation expression profiles effects of proteins on transport accessions same species unknown. Here, genes AtHKT1;1 , AtSOS1 AtNHX1 AtAVP1 determined four ecotypes Arabidopsis thaliana . Not only differentially regulated between ecotypes, levels linked concentration...
Under saline conditions, higher plants restrict the accumulation of chloride ions (Cl–) in shoot by regulating their transfer from root symplast into xylem-associated apoplast. To identify molecular mechanisms underpinning this phenomenon, we undertook a transcriptional screen salt stressed Arabidopsis (Arabidopsis thaliana) roots. Microarrays, quantitative RT-PCR, and promoter-GUS fusions identified candidate gene involved Cl– xylem loading Nitrate transporter 1/Peptide Transporter family...
Salinity tolerance is correlated with shoot chloride (Cl(-)) exclusion in multiple crops, but the molecular mechanisms of long-distance Cl(-) transport are poorly defined. Here, we characterize planta role AtSLAH1 (a homologue slow type anion channel-associated 1 (SLAC1)). This protein, localized to plasma membrane root stelar cells, has its expression reduced by salt or ABA, which key predictions for a protein involved loading into xylem. Artificial microRNA knockdown mutants had...
Stomata are tiny pores on the leaf surface that central to gas exchange. Stomatal number, size and aperture key determinants of plant transpiration photosynthesis, variation in these traits can affect growth productivity. Current methods screen for stomatal phenotypes tedious not high throughput. This impedes research biology hinders efforts develop resilient crops with optimised patterning. We have developed a rapid non-destructive method phenotype three crop species: wheat, rice tomato.The...
There is increasing evidence of the important roles glutamate receptors (GLRs) in plant development and adaptation to stresses. However, studies these putative ion channels, both planta Xenopus oocytes, may have been limited by our lack knowledge possible GLR heteromer formation plants. We developed a modification single-cell sampling technique investigate co-expression, thus potential formation, single cells Arabidopsis thaliana leaves. Micro-EXpression amplification (MEX) has allowed us...
ABSTRACT Salinity is a major abiotic stress which affects crop plants around the world, resulting in substantial loss of yield and millions dollars lost revenue. High levels Na + shoot tissue have many adverse effects and, crucially, cereals commonly inversely proportional to extent accumulation. We therefore need identify genes, resistant plant cultivars cellular processes that are involved salinity tolerance, with goal introducing these factors into commercially available crops. Through...
The accumulation of high concentrations chloride (Cl–) in leaves can adversely affect plant growth. When comparing different varieties the same Cl– sensitive species those that exclude relatively more from their shoots tend to perform better under saline conditions; however, molecular mechanisms involved maintaining low shoot remain largely undefined. Recently, it was shown NRT1/PTR Family 2.4 protein (NPF2.4) loads into root xylem, which affects Arabidopsis shoots. Here we characterize...
Novel QTL for salinity tolerance traits have been detected using non-destructive and destructive phenotyping in bread wheat were shown to be linked improvements yield saline fields. Soil is a major limitation cereal production. Breeding new salt-tolerant cultivars has the potential improve crop yields. In this study, doubled haploid mapping population, derived from bi-parental cross of Excalibur × Kukri, was grown glasshouse under control treatments evaluated high-throughput imaging...
The global need for increased food production means that agriculture is moving into regions with lower rainfall and saline soils, which occupy over 6% of the world land area (Munns, 2005). aim this workshop was to work towards completing an energy budget mechanisms salinity tolerance in crop plants, as a guide most cost-effective breeding strategies increasing salt yield important crops. discussions took account supply from mitochondria chloroplasts, demands transport water, Na+, Cl− K+,...
Bread wheat (Triticum aestivum L.) is one of the most important food crops, however it only moderately tolerant to salinity stress. To improve yield under saline conditions, breeding for improved tolerance needed. We have identified nine quantitative trail loci (QTL) different salt sub-traits in a recombinant inbred line (RIL) population, derived from bi-parental cross Excalibur × Kukri. This population was screened subtraits using combination both destructive and non-destructive...