- Plant Stress Responses and Tolerance
- Genetic Mapping and Diversity in Plants and Animals
- Plant nutrient uptake and metabolism
- Wheat and Barley Genetics and Pathology
- Genetics and Plant Breeding
- Leaf Properties and Growth Measurement
- Remote Sensing in Agriculture
- Mycorrhizal Fungi and Plant Interactions
- Plant Molecular Biology Research
- Plant responses to water stress
- Smart Agriculture and AI
- Spectroscopy and Chemometric Analyses
- Plant Micronutrient Interactions and Effects
- Rice Cultivation and Yield Improvement
- Crop Yield and Soil Fertility
- Genomics, phytochemicals, and oxidative stress
- Plant Parasitism and Resistance
- Plant Water Relations and Carbon Dynamics
- Greenhouse Technology and Climate Control
- Agriculture, Soil, Plant Science
- Plant Pathogens and Fungal Diseases
- Fungal Biology and Applications
- Legume Nitrogen Fixing Symbiosis
- Plant tissue culture and regeneration
- Silicon Effects in Agriculture
The University of Adelaide
2016-2025
Australian Centre for Plant Functional Genomics
2016-2025
Australian Wine Research Institute
2018-2023
University of Vienna
2021
Western Sydney University
2019
Australian Plant Phenomics Network
2016-2019
University of South Australia
2019
Wine Australia
2019
Cluster of Excellence on Plant Sciences
2014
University of Cologne
2004-2014
Summary Methionine‐derived glucosinolates belong to a class of plant secondary metabolites that serve as chemoprotective compounds in biotic defense reactions and also exhibit strong anticancerogenic properties beneficial human health. In screen for the trans ‐activation potential various transcription factors toward glucosinolate biosynthetic genes, we could identify HAG1 ( HIGH ALIPHATIC GLUCOSINOLATE 1 , referred MYB28 ) gene positive regulator aliphatic methionine‐derived glucosinolates....
Summary Glucosinolates are a class of plant secondary metabolites that serve as antiherbivore compounds in defence. A previously identified Arabidopsis thaliana activation‐tagged line, displaying altered levels metabolites, was shown here to be affected the content indolic and aliphatic glucosinolates. The observed chemotype caused by activation R2R3‐MYB transcription factor gene HIG1 ( HIGH INDOLIC GLUCOSINOLATE 1 , also referred MYB51 ). HIG1/MYB51 activate promoters glucosinolate...
With the establishment of advanced technology facilities for high throughput plant phenotyping, problem estimating biomass individual plants from their two dimensional images is becoming increasingly important. The approach predominantly cited in literature to estimate a as linear function projected shoot area images. However, estimation error this model, which solely area, large, prohibiting accurate plants, particularly salt-stressed plants. In paper, we propose method based on specific...
Drought is one of the most severe stresses, endangering crop yields worldwide. In order to select drought tolerant genotypes, access exotic germplasm and efficient phenotyping protocols are needed. this study high-throughput platform "The Plant Accelerator", Adelaide, Australia, was used screen a set 47 juvenile (six week old) wild barley introgression lines (S42ILs) for stress responses. The kinetics growth development evaluated under early well watered treatments. High correlation (r=0.98)...
Abstract High-throughput phenotyping produces multiple measurements over time, which require new methods of analyses that are flexible in their quantification plant growth and transpiration, yet computationally economic. Here we develop such apply this to a rice population genotyped with 700k SNP high-density array. Two diversity panels, indica aus , containing total 553 genotypes, phenotyped waterlogged conditions. Using cubic smoothing splines estimate identify four time intervals...
The use of high-throughput phenotyping systems and non-destructive imaging is widely regarded as a key technology allowing scientists breeders to develop crops with the ability perform well under diverse environmental conditions. However, many these studies have been optimized using model plant Arabidopsis thaliana. In this study, Plant Accelerator® at University Adelaide, Australia, was used investigate growth phenotypic response important cereal crop, Sorghum bicolor L. Moench related...
Abstract Background Soil salinity is an abiotic stress wide spread in rice producing areas, limiting both plant growth and yield. The development of salt-tolerant requires efficient high-throughput screening techniques to identify promising lines for salt affected areas. Advances made image-based phenotyping provide opportunity use non-destructive imaging screen tolerance traits a range germplasm reliable, quantitative way. However, the application remains limited. Results A protocol assess...
Salinity affects a significant portion of arable land and is particularly detrimental for irrigated agriculture, which provides one-third the global food supply. Rice (Oryza sativa), most important crop, salt sensitive. The genetic resources tolerance in rice germplasm exist but are underutilized due to difficulty capturing dynamic nature physiological responses stress. basis these predicted be polygenic. In an effort address this challenge, we generated temporal imaging data from 378...
Summary Cereal varieties with improved salinity tolerance are needed to achieve profitable grain yields in saline soils. The expression of AVP 1 , an A rabidopsis gene encoding a vacuolar proton pumping pyrophosphatase ( H + ‐ PP ase), has been shown improve the transgenic plants greenhouse conditions. However, potential for this yield cereal crops field yet be evaluated. Recent advances high‐throughput nondestructive phenotyping technologies also offer opportunity quantitatively evaluate...
Soil salinity results in reduced productivity chickpea. However, breeding for tolerance is challenging because of limited knowledge the key traits affecting performance under elevated salt and difficulty high-throughput phenotyping large, diverse germplasm collections. This study utilised image-based to genetic variation chickpea 245 accessions. On average plant growth rate (obtained from tracking leaf expansion through time) by 20%, height 15% shoot biomass 28%. Additionally, induced pod...
By means of yeast (Saccharomyces cerevisiae) two-hybrid screening, we identified basic helix-loop-helix transcription factor05 (bHLH05) as an interacting partner MYB51, the key regulator indolic glucosinolates (GSLs) in Arabidopsis (Arabidopsis thaliana). Furthermore, show that bHLH04, bHLH05, and bHLH06/MYC2 also interact with other R2R3-MYBs regulating GSL biosynthesis. Analysis bhlh loss-of-function mutants revealed single retained levels were similar to those wild-type plants, whereas...
Barley (Hordeum vulgare L.) is the fourth most important cereal crop worldwide. production compromised by many abiotic stresses including drought. Wild barley a valuable source of alleles that can improve adaptation cultivated to drought stress.In present study, nested association mapping population named HEB-25, consisting 1420 BC1S3 lines were developed crossing 25 different wild accessions elite cultivar 'Barke', was evaluated under both control and drought-stressed conditions in...
Wheat occupies a special role in global food security since, addition to providing 20% of our carbohydrates and protein, almost 25% the production is traded internationally. The importance wheat for was recognised by Chief Agricultural Scientists G20 group countries when they endorsed establishment Initiative 2011. tasked with supporting research community facilitating collaboration, information resource sharing helping build capacity address challenges facing an increasingly variable...
There is increasing evidence of the important roles glutamate receptors (GLRs) in plant development and adaptation to stresses. However, studies these putative ion channels, both planta Xenopus oocytes, may have been limited by our lack knowledge possible GLR heteromer formation plants. We developed a modification single-cell sampling technique investigate co-expression, thus potential formation, single cells Arabidopsis thaliana leaves. Micro-EXpression amplification (MEX) has allowed us...
The spike of a cereal plant is the grain-bearing organ whose physical characteristics are proxy measures grain yield. ability to detect and characterise spikes from 2D images plants, such as wheat, therefore provides vital information on tiller number yield potential. We have developed novel detection method for wheat plants involving, firstly, an improved colour index segmentation and, secondly, neural network-based using Laws texture energy detection. step was further by removing noise...
Crop yield in low-rainfall environments is a complex trait under multigenic control that shows significant genotype×environment (G×E) interaction. One way to understand and track this link physiological studies genetics by using imaging platforms phenotype large segregating populations. A wheat population developed from parental lines contrasting their mechanisms of maintenance water deficit was studied both an platform the field. We combined phenotyping methods common analysis pipeline...
Novel QTL for salinity tolerance traits have been detected using non-destructive and destructive phenotyping in bread wheat were shown to be linked improvements yield saline fields. Soil is a major limitation cereal production. Breeding new salt-tolerant cultivars has the potential improve crop yields. In this study, doubled haploid mapping population, derived from bi-parental cross of Excalibur × Kukri, was grown glasshouse under control treatments evaluated high-throughput imaging...
Quantifying plant water content and nitrogen levels determining phenotypes is important for crop management achieving optimal yield quality. Hyperspectral methods have the potential to advance high throughput phenotyping efforts by providing a rapid, accurate non-destructive alternative estimating biochemical physiological traits. Our study (i) acquired hyperspectral images of wheat plants using system, (ii) developed regression models capable predicting (iii) applied coefficients from...
There are a number of unresolved issues in the design experiments greenhouses. They include whether statistical designs should be used and, if so, which used. Also, there thigmomorphogenic or other effects arising from movement plants on conveyor belts within greenhouse? A two-phase, single-line wheat experiment involving four tactics was conducted conventional greenhouse and fully-automated phenotyping (Smarthouse) to investigate these issues.Analyses our show that small east-west trend...
Early vigor is an important trait for many rice ( L.)-growing environments. However, genetic characterization and improvement early hindered by the temporal nature of strong genotype × environment effects. We explored architecture shoot growth dynamics during active tillering stages applying a functional modeling genomewide association (GWAS) mapping approach on diversity panel ∼360 accessions. Multiple loci with small effects trajectory were identified, indicating complex polygenic...
Societal Impact Statement Sorghum is an important cereal crop that provides calories and nutrients for much of the world's population, it often grown with low fertiliser input. Optimising yield, nutritive content bioavailability sorghum grain minimal input importance human nutrition, arbuscular mycorrhizal (AM) fungi have previously shown potential to assist in this. Across genetic diversity, AM improved nutrition zinc iron a phosphorus soil. Thus, food production systems effectively manage...