A5014351026- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Pathogens and Resistance
- Mitochondrial Function and Pathology
- Potato Plant Research
- Plant tissue culture and regeneration
- Biochemical and Structural Characterization
- Plant Micronutrient Interactions and Effects
- Legume Nitrogen Fixing Symbiosis
- Plant responses to water stress
- Lipid metabolism and biosynthesis
- Plant Gene Expression Analysis
- Nitrogen and Sulfur Effects on Brassica
- Light effects on plants
- Phosphorus and nutrient management
- Phytase and its Applications
- Microbial Natural Products and Biosynthesis
- Plant Reproductive Biology
- Remote Sensing in Agriculture
- Genomics and Phylogenetic Studies
- Soybean genetics and cultivation
- Single-cell and spatial transcriptomics
- Plant Parasitism and Resistance
La Trobe University
2016-2025
ARC Centre of Excellence in Plant Energy Biology
2014-2025
Australian Research Council
2015-2024
AgriBio
2017-2024
Agriculture and Food
2021-2024
University of Zurich
2021
Sainsbury Laboratory
2021
University of East Anglia
2021
Norwich Research Park
2021
Australian National University
2006-2018
Summary Interactions between phytohormones play important roles in the regulation of plant growth and development, but knowledge networks controlling hormonal relationships, such as oxylipins auxins, is just emerging. Here, we report transcriptional two A rabidopsis YUCCA genes, YUC8 YUC9 , by oxylipins. Similar to previously characterized family members, show that both are involved auxin biosynthesis, demonstrated increased contents auxin‐dependent phenotypes displayed gain‐of‐function...
The transport of metal micronutrients to developing organs in a plant is mediated primarily by the sieve elements. Ligands are thought form complexes with free ions order prevent cellular damage, but no binding partners have been unequivocally identified from plants so far. This study has used phloem-mediated during germination castor bean seedling identify an iron protein (ITP). It demonstrated that essentially all 55Fe fed seedlings associated fraction phloem exudate. shown ITP carries...
Abstract The translationally controlled tumor protein (TCTP) is an important component of the TOR (target rapamycin) signaling pathway, major regulator cell growth in animals and fungi. TCTP acts as guanine nucleotide exchange factor Ras GTPase Rheb that controls activity Drosophila melanogaster. We therefore examined role Arabidopsis thaliana planta. Plant TCTPs exhibit distinct sequence differences from nonplant homologs but share key binding surface. Green fluorescent reporter lines show...
Alternative splicing (AS) of pre-mRNAs promotes transcriptome and proteome diversity plays important roles in a wide range biological processes. However, the role AS maintaining mineral nutrient homeostasis plants is largely unknown. To clarify this role, we obtained whole RNA sequencing data from rice (Oryza sativa) roots grown presence or absence several nutrients (Fe, Zn, Cu, Mn, P). Our systematic analysis revealed 13,291 alternatively spliced genes, representing ∼53.3% multiexon genes...
Variation in DNA methylation enables plants to inherit traits independently of changes sequence. Here, we have screened an Arabidopsis population epigenetic recombinant inbred lines (epiRILs) for resistance against Hyaloperonospora arabidopsidis (Hpa). These share the same genetic background, but show variation heritable patterns methylation. We identified four quantitative trait loci (epiQTLs) that provide without reducing plant growth or other (a)biotic stresses. Phenotypic...
South-western Australia harbours a global biodiversity hotspot on the world's most phosphorus (P)-impoverished soils. The greatest occurs severely nutrient-impoverished soils, where non-mycorrhizal species are prominent component of flora. Mycorrhizal dominate soils contain slightly more phosphorus. In addition to habitat loss and dryland salinity, major threat plant in this region is eutrophication due enrichment with P. Many south-western Australian extremely sensitive P, low capability...
Mitochondria adjust their activities in response to external and internal stimuli optimize growth via the mitochondrial retrograde signaling pathway. The Arabidopsis (Arabidopsis thaliana) NAC domain transcription factor ANAC017 has previously been identified as a regulator of response. We show here that overexpression leads retardation, altered leaf development with decreased cell size viability, early senescence. RNA sequencing analyses revealed increased expression higher genes related...
Phosphorus (P) is an essential macronutrient for all living organisms and limits plant growth. Four proteins comprising a single SYG1/Pho81/XPR1 (SPX) domain, SPX1 to SPX4, are putative phosphate-dependent inhibitors of Arabidopsis (Arabidopsis thaliana) PHOSPHATE STARVATION RESPONSE1 (PHR1), the master transcriptional activator phosphate starvation responses. This work demonstrated that SPX4 functions as negative regulator not only PHR1-dependent but also PHR1-independent responses in...
Seeds are a vital source of calories for humans and unique stage in the life cycle flowering plants. During seed germination, embryo undergoes major developmental transitions to become seedling. Studying gene expression individual cell types has been challenging due lack spatial information or low throughput existing methods. To overcome these limitations, transcriptomics workflow was developed germinating barley grain. This approach enabled high-throughput analysis expression, revealing...
Abstract Ser acetyltransferase (SATase; EC 2.3.1.30) catalyzes the formation of O-acetyl-Ser from l-Ser and acetyl-CoA, leading to synthesis Cys. According its position at decisive junction pathways sulfur assimilation amino acid metabolism, SATases are subject regulatory mechanisms control flux Cys synthesis. In Arabidopsis (Arabidopsis thaliana) there five genes encoding SATase-like proteins. Two isoforms, Serat3;1 Serat3;2, were characterized with respect their enzymatic properties,...
Potato late blight caused by the oomycete pathogen Phytophthora infestans can lead to immense yield loss. We investigated transcriptome of Solanum tubersoum (cv. Desiree) and characterized secretome quantitative proteomics after foliar application protective agent phosphite. also studied distribution phosphite in planta tested transgenic potato lines with impaired salicylic jasmonic acid signaling.
Phosphite is a less oxidized form of phosphorus than phosphate. considered to be taken up by the plant through phosphate transporters. It can mimic some extent, but it not metabolized into organophosphates. could therefore interfere with signalling networks. Typical physiological and transcriptional responses low availability were investigated short-term kinetics their reversion phosphite, compared phosphate, determined in both roots shoots Arabidopsis thaliana. treatment resulted strong...
Mitochondria have critical functions in the acclimation to abiotic and biotic stresses. Adverse environmental conditions lead increased demands energy supply metabolic intermediates, which are provided by mitochondrial ATP production tricarboxylic acid (TCA) cycle. also play a role as stress sensors adjust nuclear gene expression via retrograde signalling with transcription factor (TF) ANAC017 kinase CDKE1 key components integrate various signals into this pathway. To determine importance of...
Abstract In plant cells, mitochondria are ideally positioned to sense and balance changes in energy metabolism response changing environmental conditions. Retrograde signaling from the nucleus is crucial for adjusting required transcriptional responses. We show that ANAC017, master regulator of mitochondrial stress, directly recruits a cascade involving hormones ethylene auxin as well MAP KINASE (MKK) 9–MAP (MPK) 3/6 pathway Arabidopsis thaliana. Chromatin immunoprecipitation followed by...
Serine acetyltransferase (SAT) catalyzes the rate‐limiting step of cysteine biosynthesis in bacteria and plants functions association with O‐acety lserine (thiol) lyase (OAS‐TL) synthase complex. Very little is known about structure catalysis SATs except that they share a characteristic C‐terminal hexapeptide‐repeat domain number enzymatically unrelated acyltransferases. Computational modeling this was performed for mitochondrial SAT isoform from Arabidopsis thaliana , based on crystal...
Real time biomolecular interaction analysis based on surface plasmon resonance has been proven useful for studying protein-protein but not extended so far to investigate enzyme-enzyme interactions, especially as pertaining regulation of metabolic activity. We have applied BIAcore technology study the during mitochondrial cysteine biosynthesis in Arabidopsis thaliana. The association two enzyme subunits hetero-oligomeric synthase complex was investigated with respect reaction intermediate and...