A5100625515- Plant nutrient uptake and metabolism
- Plant responses to water stress
- Genomics, phytochemicals, and oxidative stress
- Glutathione Transferases and Polymorphisms
- Photosynthetic Processes and Mechanisms
- Lipid metabolism and biosynthesis
- Membrane-based Ion Separation Techniques
- Birth, Development, and Health
- Arsenic contamination and mitigation
- Ion Transport and Channel Regulation
- Zebrafish Biomedical Research Applications
- Pharmacogenetics and Drug Metabolism
- Electric Motor Design and Analysis
- Magnetic Bearings and Levitation Dynamics
- Neuropeptides and Animal Physiology
- Parathyroid Disorders and Treatments
- Genetics, Aging, and Longevity in Model Organisms
- Medicinal Plant Pharmacodynamics Research
- Marine Sponges and Natural Products
- Circular RNAs in diseases
- Nitric Oxide and Endothelin Effects
- Coral and Marine Ecosystems Studies
- Cancer, Hypoxia, and Metabolism
- bioluminescence and chemiluminescence research
- Retinoids in leukemia and cellular processes
South China Municipal Engineering Design and Research Institute (China)
2024
Australian National University
2018-2023
Australian Research Council
2010-2023
Nanchang University
2021
ARC Centre of Excellence for Transformative Meta-Optical Systems
2021
Ningbo University
2018
The University of Western Australia
2010-2013
National Sun Yat-sen University
2011
Nanjing Agricultural University
2010
ARC Centre of Excellence in Plant Energy Biology
2010
Abstract The induction of cytochrome oxidase in Saccharomyces carlsbergensis was studied by following the time course elaboration (a) hemeprotein (or hemeproteins) having heme a as prosthetic group, measured spectrophotometrically at 605 mµ, and (b) activity, cell-free extracts. kinetics investigated after removal inducer (air) separate addition cycloheximide, chloramphenicol, acriflavine different times during respiratory adaptation. results were obtained. 1. Anaerobically grown cells...
Engineering membrane transporters to achieve desired functionality is reliant on availability of experimental data informing structure-function relationships and intelligent design. Plant aquaporin (AQP) isoforms are capable transporting diverse substrates such as signaling molecules, nutrients, metalloids, gases, well water. AQPs can act multifunctional channels their transport function many factors, with few studies having assessed specific for multiple substrates.High-throughput yeast...
The intra‐ and extracellular sugar contents, the activities of sucrose‐metabolizing enzymes, metabolism [U‐14C] glucose in a pulse‐chase experiment were compared between normal osmotically stressed (by 0.6 M sorbitol) sweet potato (Ipomoea batatas) suspension cells. stress enhanced levels sucrose phosphate synthase (SPS) activity. Northern blot analysis also showed that prolonged osmotic SPS gene expression at transcriptional level. Stressed cells had higher cleaving such as alkaline...
Oxyfluorfen (OXY) is widely used in agriculture as a herbicide, resulting its continuous accumulation the environment. The presence of OXY can be detected soil and rivers. However, until now, potential toxicity to aquatic organisms has not been evaluated. In this study, zebrafish was model animal evaluate OXY-induced liver toxicity. study found that 0.25, 0.5, 1 mg/L affected early development severely damaged lipid sugar metabolism larvae. Furthermore, metabolic function disorder caused...
Previous studies have suggested that increased opioid receptor κ1 (OPRK1) and δ1 (OPRD1) methylation levels are involved in Alzheimer's disease (AD). In the present study, of two genes, µ1 (OPRM1) related nociceptin 1 (OPRL1), were analyzed for their association with AD. Gene measured using bisulfite pyrosequencing DNA samples derived from blood 51 AD patients 63 controls. The results indicated there significantly elevated promoter OPRM1 OPRL1 (OPRM1: P=0.007; OPRL1: P=2.987x10‑6)....
Plants use several pathways to synthesize phosphatidylcholine (PC), the major phospholipid of eukaryotic cells. PC has important structural and signaling roles. One pathway plants for synthesis is phospho-base methylation pathway, which forms head-group phosphocholine through triple phosphoethanolamine (PEA) catalyzed by N-methyltransferases (PEAMTs). Our understanding that its physiological importance remains limited. We recently reported disruption Arabidopsis thaliana PEAMT1/NMT1...
Abstract Arsenic is a ubiquitous environmental poison that inhibits root elongation and seed germination to variable extent depending on the plant species. To understand molecular mechanisms of arsenic resistance, genetic screen was developed isolate arsenate overly sensitive (aos) mutants from an activation-tagged Arabidopsis (Arabidopsis thaliana) population. Three aos were isolated, phenotype each demonstrated be due identical disruption plastidial LIPOAMIDE DEHYDROGENASE1 (ptLPD1), gene...
Mitochondrial lipoamide dehydrogenase is essential for the activity of four mitochondrial enzyme complexes central to oxidative metabolism. The reduction in protein amount and caused by disruption LIPOAMIDE DEHYDROGENASE2 enhanced arsenic sensitivity Arabidopsis thaliana. Both arsenate arsenite inhibited root elongation, decreased seedling size increased anthocyanin production more profoundly knockout mutants than wild-type seedlings. Arsenate also stimulated lateral formation mutants....
Phosphatidylcholine (PC) is a major membrane phospholipid and precursor for signaling molecules. Understanding its synthesis important improving plant growth, nutritional value, resistance to stress. PC complex, involving several interconnected pathways, one of which proceeds from serine-derived phosphoethanolamine form phosphocholine through three sequential phospho-base methylations catalyzed by N-methyltransferases (PEAMTs). The contribution this pathway the production growth has been...
Recombinant glutathione S-transferase 3-3 expressed in Spodoptera frugiperda (SF9) cells with the use of a baculovirus expression system was modified 1 mM-iodoacetamide. Amino acid analysis indicated that 0.79 +/- 0.15 cysteine residue per enzyme subunit. The S-carbaminomethylated protein retains GSH-conjugating activity. Glutathione iodo[14C]acetamide digested Achromobacter proteinase I and resulting peptides were separated by h.p.l.c. pooled further Staphylococcus aureus V8 proteinase....
Abstract Plant aquaporins have many more functions than just transporting water. Within the diversity of plant are isoforms capable signaling molecules, nutrients, metalloids and gases. It is established that aquaporin substrate discrimination depends on combinations factors such as solute size, pore size polarity, post-translational protein modifications. But our understanding relationships between variation in structures implications for permeability limited. High-throughput yeast-based...
Rat liver glutathione S-transferase (GST) 3-3 is composed of two identical subunits, each containing three cysteine residues, Cys-86, Cys-114 and Cys-173. We have shown previously that Cys-86 not involved in the enzymic activity GST [Hsieh, Huang, Chen, Lai & Tam (1991) Biochem, J. 278, 293-297]. At 50 degrees C, iodoacetamide can inactivate enzyme by modifying Cys-114. be protected against inhibition S-(dinitrophenyl)glutathione. Site-directed mutagenesis was used to construct mutants...
Magnetic couplings possess no mechanical contact and permit higher assembly tolerance for transmitting torque from a primary driver to follower by magnetic force. This paper investigates the effects of misalignment on transmission characteristics synchronous couplings. The can be categorized into following three types: transverse, angular combination these two misalignments. Without loss generality, an axial coupling is adopted in present because its simplicity both design fabrication....