A5101615104- Advanced Proteomics Techniques and Applications
- Cancer-related molecular mechanisms research
- Mass Spectrometry Techniques and Applications
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- MicroRNA in disease regulation
- Metabolomics and Mass Spectrometry Studies
- Ferroptosis and cancer prognosis
- Cancer, Lipids, and Metabolism
- Ovarian cancer diagnosis and treatment
- Gene expression and cancer classification
- RNA Research and Splicing
- Cancer Genomics and Diagnostics
- Machine Learning in Bioinformatics
- Circular RNAs in diseases
- Cancer-related gene regulation
- Lung Cancer Treatments and Mutations
- Peptidase Inhibition and Analysis
- Face and Expression Recognition
- Identification and Quantification in Food
- Mental Health Research Topics
- Cancer Research and Treatments
- PI3K/AKT/mTOR signaling in cancer
- Cancer Mechanisms and Therapy
- Cancer, Hypoxia, and Metabolism
Zhejiang Cancer Hospital
2015-2024
Chinese Academy of Sciences
2003-2024
Nanchang University
2019-2024
China University of Petroleum, Beijing
2024
Hainan University
2022-2024
Universitätsmedizin Göttingen
2024
Cancer Hospital of Chinese Academy of Medical Sciences
2019-2023
University of Chinese Academy of Sciences
2019-2023
Zhejiang Hisun Pharmaceutical (China)
2023
Zhejiang Chinese Medical University
2023
Abstract Many cancer cells display enhanced glycolysis and suppressed mitochondrial metabolism. This phenomenon, known as the Warburg effect, is critical for tumor development. However, how coordinate glucose metabolism through tricarboxylic acid (TCA) cycle largely unknown. We demonstrate here that phosphoglycerate kinase 1 (PGK1), first ATP-producing enzyme in glycolysis, reversibly dynamically modified with O-linked N-acetylglucosamine (O-GlcNAc) at threonine 255 (T255). O-GlcNAcylation...
Abstract The pentose phosphate pathway (PPP) plays a critical role in macromolecule biosynthesis and maintaining cellular redox homoeostasis rapidly proliferating cells. Upregulation of the PPP has been shown several types cancer. However, how is regulated to confer selective growth advantage on cancer cells not well understood. Here we show that glucose-6-phosphate dehydrogenase (G6PD), rate-limiting enzyme PPP, dynamically modified with an O-linked β-N-acetylglucosamine sugar response...
Primary and acquired drug resistance is one of the main obstacles encountered in high-grade serous ovarian cancer (HGSC) chemotherapy. Cisplatin induces DNA damage through cross-linking long integrated non-coding RNAs (lincRNAs) play an important role chemical induced DNA-damage response, which suggests that lincRNAs may be also associated with cisplatin resistance. However, mechanism acting on not well understood. Here, we showed expression lin-RECK-3, H19, LUCAT1, LINC00961, linc-CARS2-2...
Significance Protein synthesis is essential for cell growth, proliferation, and survival. Deregulation of protein an important feature in many diseases. However, how regulated under physiological pathological conditions still remains poorly understood. In this study, we report unknown mechanism the regulation cells. We demonstrate that two translation initiation factors, namely eIF4A eIF4G, are modified by a single sugar N-acetylglucosamine. Detailed analyses show modification influences...
Abstract In order to reveal the mechanisms of extreme radioresistance and DNA repair in Deinococcus radiodurans , we examined proteome changes a wild‐type strain following γ‐irradiation using two‐dimensional polyacrylamide gel electrophoresis Silver‐staining. The expression levels 26 protein spots showed significant under radiation stress. Of these spots, 21 were identified with peptide mass fingerprinting matrix‐assisted laser desorption/ionization‐time flight spectrometry after tryptic...
Abstract Lysine acetylation and succinylation are major types of protein acylation that important in many cellular processes including gene transcription, metabolism, DNA damage response. Malfunctions these post-translational modifications associated with genome instability disease higher organisms. In this study, we used high-resolution nano liquid chromatography-tandem mass spectrometry combined affinity purification to quantify the dynamic changes response ultraviolet (UV)-induced cell...
Drug resistance poses a major challenge to ovarian cancer treatment. Understanding mechanisms of drug is important for finding new therapeutic targets. In the present work, cisplatin-resistant cell line A2780-DR was established with index 6.64. The cellular accumulation cisplatin significantly reduced in cells as compared A2780 consistent general character resistance. Quantitative proteomic analysis identified 340 differentially expressed proteins between and cells, which involve diverse...
Cisplatin and other platinum-based drugs have been widely used in the treatment of ovarian cancer, but most patients acquire drug resistance that greatly compromises efficacy drugs. Understanding mechanism is important for finding new therapeutic approaches. In present study, we found expression vimentin was downregulated drug-resistant cancer cell lines A2780-DR HO-8910 as compared to their respective control cells. Overexpression cells markedly increased sensitivity cisplatin, whereas...
BACKGROUND:Wound healing in chronic diabetic mellitus is mainly associated with the management of angiogenesis. The angiogenic mechanism vascular endothelial growth factor (VEGF) has been widely studied context ulcers. aim this study was to investigate wound-healing potential curcumol streptozotocin-induced rats. MATERIAL AND METHODS:Sixty male SD (Sprague Dawley) rats were purchased and randomly assigned into four groups: a control group model treated blank ointment, high-dose group,...
A negative selection strategy was used in the present study to isolate long polyA-minus RNAs from total transcriptome and a non-coding RNA named Yiya identified. is 1.9 kb intergenic ncRNA gene mapped chromosome 1q41, well-established cancer susceptibility locus. Expression profiling revealed general regulated expression pattern of major tissues, more interestingly, identified elevated mRNA levels different cancers. Quantitative analysis further demonstrated dynamic regulation cell cycle...
MicroRNAs are a class of small noncoding RNA which play important regulatory roles in variety cancers. MiRNA-specific expression profiles have been reported for several pathological conditions. In this study, we combined large scale parallel Solexa sequencing to identify 11 up-regulated miRNAs and 19 down-regulated with computational techniques the sera ovarian cancer patients while using healthy serum as control. Among above, four (miR-22, miR-93, miR-106b, miR-451) were validated by...
HOXB‑AS3 is a long non‑coding RNA and recent studies have shown that the HOXB‑AS3‑encoded micro‑peptide was associated with progression of colon cancer tumorigenesis; however, biofunction varies in different types potential function oral squamous cell carcinoma (OSCC) still unknown. The Cancer Genome Atlas (TCGA) database searched expression patterns head neck were analyzed. Reverse transcription‑quantitative PCR western blot analysis used to measure mRNA protein level patients OSCC,...
Efficient peptide and protein identifications from data-independent acquisition mass spectrometric (DIA-MS) data typically rely on a project-specific spectral library with suitable size. Here, we describe subLib, computational strategy for optimizing the specific DIA set based comprehensive library, requiring preliminary analysis of set. Compared pan-human strategy, subLib achieved 41.2% increase in precursor 35.6% group test six colorectal tumor samples. We also applied this to 389...
Oxyfluorfen (OXY) is widely used in agriculture as a herbicide, resulting its continuous accumulation the environment. The presence of OXY can be detected soil and rivers. However, until now, potential toxicity to aquatic organisms has not been evaluated. In this study, zebrafish was model animal evaluate OXY-induced liver toxicity. study found that 0.25, 0.5, 1 mg/L affected early development severely damaged lipid sugar metabolism larvae. Furthermore, metabolic function disorder caused...
A comprehensive pan-human spectral library is critical for biomarker discovery using mass spectrometry (MS)-based proteomics. DPHL v.1, a previous built from 1,096 data-dependent acquisition (DDA) MS data of 16 human tissue types, allows quantifying 10,943 proteins. Here, we generated v.2 1,608 DDA-MS data. The included 586 acquired 18 while 1,022 files were derived v.1. thus comprises 24 sample including several cancer types (lung, breast, kidney, and prostate cancer, among others). We four...
Introduction Depression is a prevalent mental illness that primarily diagnosed using psychological and behavioral assessments. However, these assessments lack objective quantitative indices, making rapid detection challenging. In this study, we propose novel method for depression based on eye movement data captured in response to virtual reality (VR). Methods Eye was collected used establish high-performance classification prediction models. Four machine learning algorithms, namely eXtreme...
Abstract APE1 is an essential gene involved in DNA damage repair, the redox regulation of transcriptional factors (TFs) and RNA processing. overexpression common cancers correlates with poor patient survival. Stress granules (SGs) are phase-separated cytoplasmic assemblies that cells form response to environmental stresses. Precise SGs pivotal cell survival, whereas their dysregulation increasingly linked diseases. Whether engages modulating SG dynamics worthy investigation. In this study,...
Polyphyllin I (PPI) is an active component in Rhizoma Paridis, which displays extensive pharmacological antitumor activities. In a previous study, we found that polyphyllin exhibited inhibitory effects on cell growth the human ovarian cancer HO-8910PM line, as well promoting apoptosis and inhibition of migration. Furthermore, gene expression was also profiled by microarray, showed numerous genes were altered PPI; three particular note associated with tumor progression, namely, Caspase-9,...
Mass spectrometry (MS)-based protein identification depends mainly on extraction and digestion. Although sodium dodecyl sulfate (SDS) can preclude enzymatic digestion interfere with MS analysis, it is still the most widely used surfactant in these steps. To overcome disadvantages, a SDS-compatible proteomic technique for SDS removal prior to MS-based analyses was developed, namely filter-aided sample preparation (FASP).Herein, based effectiveness of deoxycholate detergent spin column, we...
Free radical scavenging effects of the cellular protein extracts from two strains Deinococcus radiodurans and Escherichia coli against O2-, H2O2 *OH were investigated by chemiluminescence (CL) methods. The D. R1 KD8301 showed higher on O2- than that E. coli. also strongly scavenged with an EC50 (50% effective concentration) 0.12 0.2 mg/mL, respectively, compared to (EC50 = 3.56 mg/mL). in generated Fenton reaction, 0.059 0.1 > 1 Results assay *OH-induced DNA damage plasmid pUC18...
Preoperative differentiation of benign and malignant tumor types is critical for providing individualized treatment interventions to improve prognosis patients with ovarian cancer. High-throughput proteomics analysis urine samples was performed identify reliable non-invasive biomarkers that could effectively discriminate between the two types.In total, 132 from 73 59 cases carcinoma were divided into C1 (training test datasets) C2 (validation dataset) cohorts. Mass spectrometry (MS) data all...