A5103250883- Immunotherapy and Immune Responses
- Acute Myeloid Leukemia Research
- Immune Cell Function and Interaction
- Chronic Myeloid Leukemia Treatments
- Cancer Immunotherapy and Biomarkers
- Hematopoietic Stem Cell Transplantation
- Cancer Cells and Metastasis
- Advanced Proteomics Techniques and Applications
- MicroRNA in disease regulation
- Cancer Research and Treatments
- Cell Adhesion Molecules Research
- Extracellular vesicles in disease
- Chronic Lymphocytic Leukemia Research
- Mass Spectrometry Techniques and Applications
- Immune cells in cancer
- T-cell and B-cell Immunology
- Mesenchymal stem cell research
- Acute Lymphoblastic Leukemia research
- CAR-T cell therapy research
- RNA modifications and cancer
- Cancer-related gene regulation
- Virus-based gene therapy research
- Genomics and Chromatin Dynamics
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Eosinophilic Disorders and Syndromes
St. Michael's Hospital
2025
Unity Health Toronto
2025
Toronto Metropolitan University
2025
Huazhong University of Science and Technology
2013-2023
Union Hospital
2013-2023
Westlake University
2020
Wuhan Union Hospital
2009-2020
Union Hospital
2008-2019
Chunghwa Telecom (Taiwan)
2018
University of Michigan
2012-2015
Abstract Most studies of cancer stem cells (CSC) involve the inoculation from human tumors into immunosuppressed mice, preventing an assessment on immunologic interactions and effects CSCs. In this study, we examined vaccination produced by CSC-enriched populations histologically distinct murine after their different syngeneic immunocompetent hosts. Enriched CSCs were immunogenic more effective as antigen source than unselected tumor in inducing protective antitumor immunity. Immune sera...
Human mesenchymal stem cells (MSCs) have an intrinsic property for homing towards tumor sites and can be used as tumor-tropic vectors therapy. But very limited studies investigated the antitumor properties of MSCs themselves. In this study we antiglioma two easily accessible MSCs, namely, human adipose tissue-derived (ASCs) umbilical cord-derived (UC-MSCs). We found (1) MSC conditioned media significantly inhibit growth U251 glioma cell line; (2) induce apoptosis in (3) real-time PCR...
Immune cells in tumor microenvironment play a prominent role progression and metastasis. MicroRNA-155 (miR-155) represents an important player innate adaptive immunity by regulating differentiation, maturation activation of macrophages, dendritic cells, B T cells. However, the miR-155 expression immune solid development is less elucidated. Our current study showed that both B16-F10 melanoma Lewis lung carcinoma tumors grew much faster bic/miR-155 knockout (miR-155(-/-) ) mice along with...
The inability to target cancer stem cells (CSC) may be a significant factor contributing treatment failure. We have developed strategy the CSC populations in melanoma and squamous cell carcinoma using lysate-pulsed dendritic (DCs). CSC-DC vaccine was administered adjuvant setting after localized radiation therapy of established tumors. Using mouse models we demonstrated that DCs pulsed with CSCs enriched by virtue their expression marker ALDH (termed CSC-DC) significantly inhibited tumor...
Abstract: Purpose : Regulatory T cells (T‐reg) that control harmful autoimmune in the periphery may also suppress immune response against cancer. In this study we investigated possible involvement of CD4 + CD25 high T‐reg impairment patients with acute myeloid leukemia (AML). Experimental design The frequencies and phenotypes peripheral blood AML were determined by flow cytometry. To assess functional activity cells, , − sorted from mononuclear FACS Vantage. immunoregulatory properties...
We have previously reported that adoptive transfer of tumor-draining lymph node (TDLN) B cells confers tumor regression in a spontaneous pulmonary metastasis mouse model breast cancer. In this study, we identified IL-10-producing within these cells, and found IL-10 removal, either by using IL-10(-/-) TDLN or systemic neutralization IL-10, significantly augmented the therapeutic efficacy adoptively transferred cells. Depletion B-cell transfers increased CTLs activity PBMCs splenic recipient....
Abstract Dendritic cell (DC)-based vaccine strategies aimed at targeting cancer stem–like cells (CSC) may be most efficacious if deployed in the adjuvant setting. In this study, we offer preclinical evidence that is case for a CSC-DC as tested murine models of SCC7 squamous and D5 melanoma. Vaccination mice with an ALDHhigh after surgical excision established tumors reduced local tumor relapse prolonged host survival. This effect was augmented significantly by simultaneous administration...
Abstract Integrin β4 (ITGB4) has been shown to play an important role in the regulation of cancer stem cells (CSC). Immune targeting ITGB4 represents a novel approach target this cell population, with potential clinical benefit. We developed two immunologic strategies ITGB4: protein–pulsed dendritic (ITGB4-DC) for vaccination and adoptive transfer anti-CD3/anti-ITGB4 bispecific antibody (ITGB4 BiAb)–armed tumor-draining lymph node T cells. Two immunocompetent mouse models were utilized...
To address the increasing need for detecting and validating protein biomarkers in clinical specimens, mass spectrometry (MS)-based targeted proteomic techniques, including selected reaction monitoring (SRM), parallel (PRM), massively data-independent acquisition (DIA), have been developed. For optimal performance, they require fragment ion spectra of peptides as prior knowledge. In this report, we describe a MS pipeline spectral resource to support proteomics studies human tissue samples....
Abstract We wanted to demonstrate the value of multiparameter flow cytometry in detecting human tumor cells breast cancer (BC) (SKBR‐3) normal peripheral blood. In addition, we investigated a cluster patients compare overall survival (OS) between advanced BC [circulating (CTCs) ≥5 group] and limited (CTCs <5 group). SKBR‐3 were serially diluted whole blood sensitivity for CTCs, also compared specificity with reverse transcriptase polymerase chain reaction (RT‐PCR) method. On other hand,...
The tyrosine kinase domain (TKD) mutations of receptor C-KIT are associated with a poor prognosis in acute myeloid leukemia (AML). However, the underlying mechanisms not fully understood. We found activity protein phosphatase 2A (PP2A), human tumor suppressor whose dysfunction contributes to malignant cell behavior, was significantly decreased AML subgroups harboring C-KIT/D816V and line Kasumi-1 bearing C-KIT/N822K mutation. Primary cells various lines were treated PP2A activator FTY720....
In antitumor immunity, dendritic cells (DCs) capture, process, and present tumor antigens to T cells, initiating a tumoricidal response. However, DCs are often dysfunctional due their exposure the microenvironment (TME), leading escape from immune surveillance. Here, vital role of microRNA-155 (miR-155) in regulating function breast cancer is reported. Host miR-155 deficiency enhanced growth mice, accompanied by reduced tumors draining lymph nodes. impaired maturation, migration ability,...
The performance of data-independent acquisition (DIA) mass spectrometry (MS) depends on the separation efficiency peptide precursors. In Orbitrap-based spectrometers, precursors is limited by relatively slow scanning rate compared to time flight (TOF)-based MS. Here, we present PulseDIA, a multi-injection gas-phase fractionation (GPF) strategy for enhanced DIA-MS. This achieved equally dividing conventional DIA analysis covering entire range into multiple injections analyses with...
ABSTRACT Although previous lentivirus vector systems have used human immunodeficiency virus type 1 (HIV-1), HIV-2 is less pathogenic in humans and amenable to pathogenicity testing a primate model. In this study, an molecular clone that infectious but apathogenic macaques was first define cis -acting regions can be deleted prevent genomic encapsidation replication without inhibiting viral gene expression. Lentivirus determinants are complex incompletely defined; for HIV-2, some deletions...
Bmi1 is a polycomb group proto-oncogene that has been implicated in multiple tumor types. However, its role hepatocellular carcinoma (HCC) development not well studied. In this article, we report overexpressed human HCC samples. When expression knocked down cell lines, it significantly inhibits proliferation and perturbs cycle regulation. To investigate the of promoting liver cancer vivo, stably expressed and/or an activated form Ras (RasV12) mouse liver. We found while or RasV12 alone...
Kruppel-like factor 4 (KLF4) is a transcription that highly expressed in differentiated epithelial cells including of the skin. It critical for specification or function cells. Moreover, KLF4 functions either as tumor suppressor an oncogene depending on different cellular contexts. However, role skin tumorigenesis remains controversial. To address this issue, we first examined expression using cohort samples from patients with squamous cell carcinoma and basal found 21 24 tissues (87.5%),...
Circulating tumor stem cells (CTSC), a subpopulation of circulating (CTC), may lead to recurrent diseases. The aim this study was detect CTC (CD45−EpCAM+) and CTSC (CD45−EpCAM+CD44+CD24−) breast cancer (BC) patients, as well explore their clinical relevance. in peripheral blood (PB) 45 female BC patients were detected by using flow cytometry (FCM). SKBR-3 mixed with MNC four healthy volunteers at different ratios order evaluate the sensitivity FCM. Real-time quantitative polymerase chain...
Purpose To rapidly identify protein abundance changes in biopsy‐level fresh‐frozen hepatocellular carcinoma (HCC). Experimental design The pressure‐cycling technology (PCT) is applied and sequential window acquisition of all theoretical mass spectra (SWATH‐MS) workflow optimized to analyze 38 tissue samples from 19 HCC patients. Each proteome analyzed with 45 min LC gradient. MCM7 validated using immunohistochemistry (IHC). Results A total 11 787 proteotypic peptides 2579 SwissProt proteins...
Liver cancer is among the top leading causes of mortality worldwide. Particularly, hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (CCA) have been extensively investigated from aspect tumor biology. However, a comprehensive systematic understanding molecular characteristics HCC CCA remains absent. Here, we characterized proteome landscapes using data-independent acquisition (DIA) mass spectrometry (MS) method. By comparing quantitative proteomes CCA, found several...
Efficient peptide and protein identifications from data-independent acquisition mass spectrometric (DIA-MS) data typically rely on a project-specific spectral library with suitable size. Here, we describe subLib, computational strategy for optimizing the specific DIA set based comprehensive library, requiring preliminary analysis of set. Compared pan-human strategy, subLib achieved 41.2% increase in precursor 35.6% group test six colorectal tumor samples. We also applied this to 389...