A5030860036- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Advanced Cellulose Research Studies
- Biofuel production and bioconversion
- Plant Gene Expression Analysis
- Phytoplasmas and Hemiptera pathogens
- Genetic Mapping and Diversity in Plants and Animals
- Wheat and Barley Genetics and Pathology
- Genetics and Plant Breeding
- Algal biology and biofuel production
- Plant Reproductive Biology
- Plant Disease Resistance and Genetics
- Fermentation and Sensory Analysis
- Chromosomal and Genetic Variations
- Advanced ceramic materials synthesis
- Lignin and Wood Chemistry
- Venous Thromboembolism Diagnosis and Management
- Aluminum Alloys Composites Properties
- Advanced materials and composites
- Plant Disease Management Techniques
- Magnetic and Electromagnetic Effects
- Biocrusts and Microbial Ecology
- Cocoa and Sweet Potato Agronomy
- Plant Water Relations and Carbon Dynamics
University of Manchester
2014-2024
John H. Stroger, Jr. Hospital of Cook County
2024
Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu
2024
Chaudhary Charan Singh University
2020-2023
Amity University
2023
Bharat Heavy Electricals (India)
1991-2020
All India Institute of Medical Sciences Raipur
2019
All India Institute of Medical Sciences
2010-2019
All India Institute of Medical Sciences Bhopal
2019
Umeå Plant Science Centre
2011-2018
Trees represent the largest terrestrial carbon sink and a renewable source of ligno-cellulose. There is significant scope for yield quality improvement in these largely undomesticated species, efforts to engineer elite varieties will benefit from improved understanding transcriptional network underlying cambial growth wood formation. We generated high-spatial-resolution RNA sequencing data spanning secondary phloem, vascular cambium, wood-forming tissues Populus tremula The transcriptome...
The transcription factor MYB103 was previously identified as a member of the transcriptional network regulating secondary wall biosynthesis in xylem tissues Arabidopsis, and proposed to act on cellulose biosynthesis. It is direct target SECONDARY WALL ASSOCIATED NAC DOMAIN PROTEIN 1 (SND1), 35S-driven dominant repression or over-expression modifies thickness. We two myb103 T-DNA insertion mutants chemically characterized their lignocellulose by pyrolysis/GC/MS, 2D NMR, FT-IR...
The woody tissue of trees is composed xylem cells that arise from divisions stem within the cambial meristem. rate cell formation dependent upon division cambium and controlled by both genetic environmental factors. In annual plant Arabidopsis, signaling between a peptide ligand CLE41 receptor kinase PXY controls divisions; however, pathway regulating secondary growth in has not been identified. Here, we show an aspen PttPXY its PttCLE41 are functional orthologs act to control...
One of the defining characteristics plant growth and morphology is pivotal role cell expansion. While mechanical properties wall determine both extent direction expansion, cortical microtubule array plays a critical in organization and, consequently, determining directional (anisotropic) The microtubule-severing enzyme katanin essential for plants to form aligned arrays; however, increasing severing activity alone not sufficient drive alignment. Here, we demonstrate that depends upon...
Several key enzymes in lignin biosynthesis of Populus have been down-regulated by transgenic approaches to investigate their role wood lignification and explore potential for modification. Cinnamate 4-hydroxylase is an enzyme the early phenylpropanoid pathway that has not yet functionally analyzed Populus. This study shows down-regulation cinnamate reduced Klason content 30% with no significant change syringyl guaiacyl ratio. The reduction resulted ultrastructural differences a 10% decrease...
Summary Sucrose is the main transported form of carbon in several plant species, including Populus species. metabolism developing wood has therefore a central role partitioning to stem biomass. Half sucrose‐derived fructose, but fructose received little attention as factor walls cells. We show that RNAi‐mediated reduction FRK2 activity hybrid aspen ( tremula × tremuloides ) led accumulation soluble neutral sugars and decrease hexose phosphates UDP‐glucose, indicating flux cell‐wall...
Plant cellulose microfibrils are synthesized by a process that propels the synthase complex (CSC) through plane of plasma membrane. How interactions between membranes and CSC regulated is currently unknown. Here, we demonstrate all catalytic subunits CSC, known as A (CESA) proteins, S-acylated. Analysis Arabidopsis CESA7 reveals four cysteines in variable region 2 (VR2) two at carboxy terminus (CT) S-acylation sites. Mutating both VR2 CT permits assembly trafficking to Golgi but prevents...
The biosynthesis of wood in aspen (Populus) depends on the metabolism sucrose, which is main transported form carbon from source tissues. largest fraction biomass cellulose, synthesized UDP-glucose. Sucrose synthase (SUS) has been proposed previously to interact directly with cellulose complexes and specifically supply UDP-glucose for biosynthesis. To investigate role SUS biosynthesis, we characterized transgenic lines hybrid strongly reduced activity developing wood. No dramatic growth...
Abstract We have identified a gene, denoted PttMAP20, which is strongly up-regulated during secondary cell wall synthesis and tightly coregulated with the wall-associated CESA genes in hybrid aspen (Populus tremula × tremuloides). Immunolocalization studies affinity-purified antibodies specific for PttMAP20 revealed that protein found all types developing xylem it most abundant cells forming walls. This sequence contains highly conserved TPX2 domain first microtubule-associated (MAP) Xenopus...
Lignocellulosic biomass is an important renewable resource for biofuels and materials. How plants synthesise cellulose not completely understood. It known that synthase complex (CSCs) moving in the plasma membrane cellulose. CESA proteins are core components of CSC. In Arabidopsis, vitro mutagenesis followed by complementation analysis mutants lacking gene represents tool studying any biological process, including biosynthesis. Analysis a large number crucial these types studies.By using...
The cellulose synthase complex (CSC) exhibits a 6-fold symmetry and is known as “rosette.” Each CSC believed to contain between 18 24 CESA proteins that each synthesize an individual glucan chain. These chains form the microfibrils confer remarkable structural properties of cellulose. At least three different classes are essential CSC. However, while organization determines microfibril structure, how organized within remains unclear. Parts plant map sufficiently well onto bacterial (BcsA)...
In order to find out optimum culture condition for algal growth, the effect of light irradiance and temperature on growth rate, biomass composition pigment production Spirulina platensis were studied in axenic batch cultures. Growth kinetics cultures showed a wide range tolerance from 20 °C 40 °C. Maximum cell with maximum accumulation chlorophyll phycobilliproteins found at 35 2,000 lux intensity. But further increase intensity, reduction rate was observed. Carotenoid content 3,500 lux....
Cellulose is synthesized at the plasma membrane by cellulose synthase complexes (CSCs) containing synthases (CESAs). Genetic analysis and CESA isoform quantification indicate that in secondary cell walls of Arabidopsis (Arabidopsis thaliana) isoforms CESA4, CESA7, CESA8 equimolar amounts. Here, we used quantitative proteomics to investigate whether CSC model based on wall stoichiometry can be applied angiosperm tree aspen (Populus tremula) gymnosperm Norway spruce (Picea abies). In...
Cellulose microfibrils are the basic units of cellulose in plants. The structure these is at least partly determined by synthase complex. In higher plants, this complex composed 18 to 24 catalytic subunits known as CELLULOSE SYNTHASE A (CESA) proteins. Three different classes CESA proteins required for synthesis and secondary cell wall biosynthesis represented CESA4, CESA7, CESA8. To probe relationship between microfibril structure, we created mutant cesa that lack activity but retain...
Wood formation in trees requires carbon import from the photosynthetic tissues. In several tree species, including Populus majority of this is derived sucrose (Suc) transported phloem. The mechanism radial Suc transport phloem to developing wood not well understood. We investigated role active during secondary cell wall hybrid aspen (Populus tremula × tremuloides). show that RNA interference-mediated reduction PttSUT3 (for Suc/H+ symporter) caused thinner fiber walls accompanied by a...