A5108107054- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Biofuel production and bioconversion
- Fermentation and Sensory Analysis
- Polysaccharides and Plant Cell Walls
- Tree Root and Stability Studies
- Photosynthetic Processes and Mechanisms
- Marine and coastal ecosystems
- Postharvest Quality and Shelf Life Management
- Lignin and Wood Chemistry
- Plant Reproductive Biology
- Heavy metals in environment
- Metabolomics and Mass Spectrometry Studies
- Wood Treatment and Properties
- Advanced Cellulose Research Studies
- Wood and Agarwood Research
- Isotope Analysis in Ecology
- Soil and Water Nutrient Dynamics
- Plant Stress Responses and Tolerance
- Plant Water Relations and Carbon Dynamics
- Mass Spectrometry Techniques and Applications
- Distributed and Parallel Computing Systems
- Proteins in Food Systems
- Plant Surface Properties and Treatments
- Algal biology and biofuel production
Swedish University of Agricultural Sciences
2011-2022
Umeå Plant Science Centre
2011-2022
Genome Institute of Singapore
2019-2020
Chalmers University of Technology
2014
KTH Royal Institute of Technology
2010
Max Planck Institute of Colloids and Interfaces
2010
Wallenberg Wood Science Center
2010
University of Southampton
2010
Purdue University West Lafayette
1930
Abstract The hemicellulose glucuronoxylan (GX) is a major component of plant secondary cell walls. However, our understanding GX synthesis remains limited. Here, we identify and analyze two new genes from Arabidopsis (Arabidopsis thaliana), IRREGULAR XYLEM9-LIKE (IRX9-L) IRX14-LIKE (IRX14-L) that encode glycosyltransferase family 43 members proposed to function during xylan backbone elongation. We place IRX9-L IRX14-L in genetic framework with six previously described (IRX9, IRX10, IRX10-L,...
Abstract Postmortem lignification of xylem tracheary elements (TEs) has been debated for decades. Here, we provide evidence in Zinnia elegans TE cell cultures, using pharmacological inhibitors and intact Z. plants Fourier transform infrared microspectroscopy, that occurs postmortem (i.e., after programmed death). In situ RT-PCR verified expression the lignin monomer biosynthetic cinnamoyl CoA reductase cinnamyl alcohol dehydrogenase not only lignifying TEs but also unlignified non-TE cells...
The transcription factor MYB103 was previously identified as a member of the transcriptional network regulating secondary wall biosynthesis in xylem tissues Arabidopsis, and proposed to act on cellulose biosynthesis. It is direct target SECONDARY WALL ASSOCIATED NAC DOMAIN PROTEIN 1 (SND1), 35S-driven dominant repression or over-expression modifies thickness. We two myb103 T-DNA insertion mutants chemically characterized their lignocellulose by pyrolysis/GC/MS, 2D NMR, FT-IR...
Several key enzymes in lignin biosynthesis of Populus have been down-regulated by transgenic approaches to investigate their role wood lignification and explore potential for modification. Cinnamate 4-hydroxylase is an enzyme the early phenylpropanoid pathway that has not yet functionally analyzed Populus. This study shows down-regulation cinnamate reduced Klason content 30% with no significant change syringyl guaiacyl ratio. The reduction resulted ultrastructural differences a 10% decrease...
Summary Ethylene Response Factors (ERFs) are a large family of transcription factors that mediate responses to ethylene. affects many aspects wood development and is involved in tension formation. Thus ERFs could be key players connecting ethylene action development. We identified 170 gene models encoding the Populus trichocarpa genome. The transcriptional ERF genes treatments were determined stem tissues hybrid aspen ( tremula × tremuloides ) by qPCR. Selected ethylene‐responsive...
Summary Sucrose is the main transported form of carbon in several plant species, including Populus species. metabolism developing wood has therefore a central role partitioning to stem biomass. Half sucrose‐derived fructose, but fructose received little attention as factor walls cells. We show that RNAi‐mediated reduction FRK2 activity hybrid aspen ( tremula × tremuloides ) led accumulation soluble neutral sugars and decrease hexose phosphates UDP‐glucose, indicating flux cell‐wall...
The biosynthesis of wood in aspen (Populus) depends on the metabolism sucrose, which is main transported form carbon from source tissues. largest fraction biomass cellulose, synthesized UDP-glucose. Sucrose synthase (SUS) has been proposed previously to interact directly with cellulose complexes and specifically supply UDP-glucose for biosynthesis. To investigate role SUS biosynthesis, we characterized transgenic lines hybrid strongly reduced activity developing wood. No dramatic growth...
The secondary walls of angiosperms contain large amounts glucuronoxylan that is thought to be covalently linked lignin via ester bonds between 4-O-methyl-α-d-glucuronic acid (4-O-Me-GlcA) moieties in and alcohol groups lignin. This linkage proposed hydrolysed by glucuronoyl esterases (GCEs) secreted wood-degrading fungi. We report effects overexpression a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, hybrid aspen (Populus tremula L. x tremuloides Michx.) on wood...
The environmental neurotoxin β-N-methylamino-L-alanine (BMAA) is suggested to be linked with neurodegenerative disease. In a rat model, neonatal exposure BMAA induced selective uptake in the hippocampus and caused cell loss, mineralization astrogliosis as well learning memory impairments adulthood. Moreover, resulted increased protein ubiquitination cornus ammonis 1 (CA1) region of adult indicating that may induce aggregation. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) based...
Abstract. The composition of sediment organic matter (OM) exerts a strong control on biogeochemical processes in lakes, such as those involved the fate carbon, nutrients and trace metals. While between-lake spatial variability OM quality is increasingly investigated, we explored this study how molecular varies spatially within single lake related to physical parameters elemental geochemistry. Surface samples (0–10 cm) from 42 locations Härsvatten – small boreal forest with complex basin...
The secondary cell wall (SCW) in the xylem is one of largest sink organs carbon woody plants, and considered a promising sustainable bioresource for biofuels biomaterials. To enhance SCW formation poplar (Populus sp.) xylem, we developed self-reinforced system SCW-related transcription factors from Arabidopsis thaliana, involving VASCULAR-RELATED NAC-DOMAIN7 (VND7), SECONDARY WALL-ASSOCIATED NAC-DOMAIN PROTEIN 1/NAC WALL THICKENING-PROMOTING FACTOR3 (SND1/NST3), MYB46. In this system, these...
High-throughput analytical techniques to assess the chemistry of lignocellulosic plant material are crucial cell-wall research. We have established an platform for this purpose and demonstrated its usefulness with two applications. The system is based on pyrolysis, coupled gas chromatography/mass spectrometry - a technique particularly suited analysis lignocellulose. Automated multivariate-based data-processing methods used obtain results within few hours after analysis, experimental batch...
We have developed a multistep strategy that integrates data from several large-scale experiments suffer systematic between-experiment variation. This removes such variation would otherwise mask differences of interest. It was applied to the evaluation wood chemical analysis 736 hybrid aspen trees: wild-type controls and transgenic trees potentially involved in formation. The were grown four different greenhouse imposing significant between experiments. Pyrolysis coupled gas...
Mediator is a multiprotein transcriptional co-regulator complex composed of four modules; Head, Middle, Tail, and Kinase. It conveys signals from promoter-bound regulators to RNA polymerase II thus plays an essential role in eukaryotic gene regulation. We describe subunit localization activities Arabidopsis through metabolome transcriptome analyses set mutants. Functional metabolomic analysis based on the metabolite profiles mutants using multivariate statistical heat-map visualization shows...
Cyanobacteria play a pivotal role as the primary producer in many aquatic ecosystems. The knowledge on interacting processes of cyanobacteria with its environment - abiotic and biotic factors is still very limited. Many potential exocytoplasmic proteins model unicellular cyanobacterium Synechocystis PCC 6803 have unknown functions their study essential to improve our understanding this photosynthetic organism for biotechnology use. Here we characterize deletion mutant 6803, Δsll1783, strain...
Background The phytohormone ethylene (ET) has the potential to regulate secondary growth of plants and wood formation in trees. Application exogenous or its planta precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), forming tissues hybrid aspen (Populus tremula x Populus tremuloides) enhances xylem [1]. In same study it was demonstrated that stimulation enhanced (tension wood, TW) at upper side leaning stems is mediated by endogenous ET. production ET TW further supported increase ACC...
Here, we demonstrate the use of time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS) imaging data for estimation cell wall thickness in wood samples. Current research forest biotechnology focuses on transgenic trees with properties tailored to specific applications. Appropriate analytical methods characterize very heterogeneous material are constantly being developed and improved. Chemical by ToF‐SIMS represents an interesting tool this purpose many In addition chemistry, impact genetic...
The expression of