A5080720192- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Natural Compound Pharmacology Studies
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Metabolomics and Mass Spectrometry Studies
- Photosynthetic Processes and Mechanisms
- Plant and animal studies
- Berberine and alkaloids research
- Growth and nutrition in plants
- Forest Insect Ecology and Management
- Chemical synthesis and alkaloids
- Plant Stress Responses and Tolerance
- Microbial Metabolic Engineering and Bioproduction
- Phytochemistry and Biological Activities
- Insect-Plant Interactions and Control
- Ecology and Vegetation Dynamics Studies
- Phytochemistry Medicinal Plant Applications
- Polysaccharides and Plant Cell Walls
- Seed Germination and Physiology
- Plant Parasitism and Resistance
- Plant-based Medicinal Research
- Banana Cultivation and Research
- Plant Surface Properties and Treatments
- Mycorrhizal Fungi and Plant Interactions
University of Göttingen
2020-2024
Umeå Plant Science Centre
2013-2023
Swedish University of Agricultural Sciences
2014-2023
James Hutton Institute
2020
Universidade Estadual de Campinas (UNICAMP)
2003-2014
Umeå University
2008-2014
Hospital de Clínicas da Unicamp
2006-2009
Universidade Federal de Lavras
2002
Coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. The genus Coffea belongs to Rubiaceae family which includes other important plants. contains about 100 species but commercial production based only two species, arabica canephora that represent 70 % 30 total coffee market, respectively. Brazilian Genome Project was designed with objective making modern genomics resources available scientific community, working different aspects...
Arbuscular mycorrhizas (AM) are the most common symbiotic associations between a plant's root compartment and fungi. They provide nutritional benefit (mostly inorganic phosphate [Pi]), leading to improved growth, nonnutritional benefits, including defense responses environmental cues throughout host plant, which, in return, delivers carbohydrates symbiont. However, how transcriptional metabolic changes occurring leaves of AM plants differ from those induced by Pi fertilization is poorly...
SUMMARY Land plants constantly respond to fluctuations in their environment. Part of response is the production a diverse repertoire specialized metabolites. One foremost sources for metabolites relevant environmental responses phenylpropanoid pathway, which was long thought be land‐plant‐specific adaptation shaped by selective forces terrestrial habitat. Recent data have, however, revealed that streptophyte algae, algal relatives land plants, have candidates genetic toolkit biosynthesis and...
Associations of salicylate-like phenolic glycosides (PGs) with biological activity have been reported in Salix and Populus trees, but only for a few compounds, relation to limited number herbivores. By considering the full diversity PGs, we may improve our ability recognize genotypes or chemotype groups enhance understanding their ecological function. Here, present fast efficient general method salicylate determination leaves Eurasian aspen that uses ultra-high performance liquid...
Wood, or secondary xylem, is the product of xylogenesis, a developmental process that begins with proliferation cambial derivatives and ends mature xylem fibers vessels lignified cell walls. Fully has undergone series cellular processes, including division, expansion, wall formation, lignification programmed death. A complex network interactions between transcriptional regulators signal transduction pathways controls wood formation. However, role metabolites during this not been...
Xylem sap is the major transport route for nutrients from roots to shoots. In present study, we investigated how variations in nitrogen (N) nutrition affected metabolome and proteome of xylem growth endophyte Brennaria salicis, also report transcriptional re-wiring leaf defenses poplar (Populus × canescens). We supplied poplars with high, intermediate or low concentrations ammonium nitrate. identified 288 unique proteins sap. Approximately 85% were shared among ammonium- nitrate-supplied...
Salicinoids are well-known defense compounds in salicaceous trees and careful screening at the population level is warranted to fully understand their diversity function. European aspen, Populus tremula, a foundation species Eurasia highly polymorphic Sweden. We exhaustively surveyed 102 replicated genotypes from Swedish Aspen collection (SwAsp) for foliar salicinoids using UHPLC-ESI-TOF/MS identified nine novel compounds, bringing total 19 this species. Salicinoid structure followed modular...
The plant cell wall plays an important role in damage-associated molecular pattern-induced resistance to pathogens and herbivorous insects. Our current understanding of wall-mediated is largely based on the degree pectin methylesterification. However, little known about acetylesterification immunity. This study describes how one pectin-modifying enzyme,
Secretory Carrier-Associated Membrane Proteins (SCAMPs) are highly conserved 32–38 kDa proteins that involved in membrane trafficking. A systems approach was taken to elucidate function of SCAMPs wood formation Populus trees. Phenotypic and multi-omics analyses were performed woody tissues transgenic trees carrying an RNAi construct for tremula x tremuloides SCAMP3 (PttSCAMP3; Potri.019G104000). The the displayed increased amounts both polysaccharides lignin oligomers, indicating deposition...
Transcriptome, metabolome and histological profiling were performed on normal aberrant somatic embryo germinants of Norway spruce (Picea abies L. Karst) providing a simplistic systems biology description conifer germination. Aberrant (AGs) formed periderm-like tissue at the apical pole lacked shoot growth above cotyledons. Transcriptome (RNA-Sequencing) revealed total 370 differentially expressed genes ≥1 or ≤-1 log2-fold change, where 92% down-regulated in AGs compared with (NGs). Genes...
ABSTRACTIn this study, ethanol-water extracts of pequi fruit peel were fractionated in order to identify and quantify the major antioxidant present it. The fractions subjected liquid-liquid phase extraction silica-gel column chromatography, activity was monitored using 2,2-diphenyl-1-picrylhydrazyl radical-scavenging assay. purity evaluated thin-layer chromatography high-performance liquid (HPLC). substance with property identified through analysis a chromatography-mass spectroscopy...
Sexual reproduction in Charophyceae abounds complex traits. Their gametangia develop as intricate structures, with oogonia spirally surrounded by envelope cells and richly pigmented antheridia. The red—probably protectant—pigmentation of antheridia is conserved across Charophyceae. Chara tomentosa is, however, unique exhibiting this pigmentation also vegetative tissue. Here, we investigated the two sympatric species, C. baltica , compared their molecular chassis for pigmentation. Using...
Abstract Pilocarpus microphyllus (Rutaceae), popularly known as jaborandi, is the only commercial source of an imidazole alkaloid named pilocarpine. In present study, variation in profile alkaloids different seasons and parts P . plant during summer was analyzed by electrospray ionization mass spectrometry positive ion mode [ESI(+)‐MS]. The fingerprints these extracts repeatedly presented similar ions which were mass‐selected studied tandem (ESI‐MS/MS ESI‐MS/MS/MS) high‐resolution...
Pilocarpine, an important imidazole alkaloid, is extracted from the leaves of Pilocarpus microphyllus (Rutaceae), known in Brazil as jaborandi and used mainly for treatment glaucoma. Jaborandi also contain other alkaloids, whose pharmacological physiological properties are unknown, biosynthetic pathways under investigation. In present study, a HPLC method coupled with ESI-MSn was developed their qualitative quantitative analysis. This permits chromatographic separation alkaloids found...
Abstract Background Bioconversion of wood into bioproducts and biofuels is hindered by the recalcitrance woody raw material to bioprocesses such as enzymatic saccharification. Targeted modification chemical composition feedstock can improve saccharification but this gain often abrogated concomitant reduction in tree growth. Results In study, we report on transgenic hybrid aspen ( Populus tremula × tremuloides ) lines that showed potential increase biomass production both greenhouse after 5...
1 Ecosystem retrogression, the decline-phase of ecosystem development, occurs during long-term absence catastrophic disturbance. It usually involves increased nutrient limitation over time, and leads to reductions in primary productivity, decomposition, cycling. 2 As a consequence, retrogression can alter quality abundance host plants as food resources, but little is known about how these changes influence herbivore densities foliage consumption. 3 In this study, we used 5000-year-old...
Summary Wood is the most important repository of assimilated carbon in biosphere, form large polymers (cellulose, hemicelluloses including glucuronoxylan, and lignin) that interactively a composite, together with soluble extractives phenolic aliphatic compounds. Molecular interactions among these compounds are not fully understood. We have targeted expression fungal α‐glucuronidase to wood cell wall aspen ( Populus tremula L. × tremuloides Michx.) Arabidopsis thaliana (L.) Heynh), decrease...
Mediator is a multiprotein transcriptional co-regulator complex composed of four modules; Head, Middle, Tail, and Kinase. It conveys signals from promoter-bound regulators to RNA polymerase II thus plays an essential role in eukaryotic gene regulation. We describe subunit localization activities Arabidopsis through metabolome transcriptome analyses set mutants. Functional metabolomic analysis based on the metabolite profiles mutants using multivariate statistical heat-map visualization shows...
Photosynthetic organisms strictly depend on CO2 availability and the CO2:O2 ratio, as both CO2/O2 compete for catalytic site of Rubisco. Green alga Chlamydomonas reinhardtii, can overcome shortage by inducing CO2-concentrating mechanism (CCM). Cells transferred to low-CO2 are subjected light-driven oxidative stress due decrease in electron sink. Response environmental perturbations is mediated some extent changes lipid carotenoid metabolism. We thus hypothesize that when cells challenged...
Pilocarpine is an imidazol alkaloid exclusively found in Pilocarpus genus and P. microphyllus accumulates its highest content the leaves. There no report literature on variability of pilocarpine this genus. A population 20 genotypes from state Maranhão, Brazil, was analyzed for Random Amplification Polymorphic DNA (RAPD) markers content. Although it not possible to establish any correlation between these features, absence or presence some could indicate a association with alkaloid.