A5020655823- Pancreatic function and diabetes
- Advanced Drug Delivery Systems
- Platelet Disorders and Treatments
- Complement system in diseases
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Diabetes and associated disorders
- Nanoparticle-Based Drug Delivery
- Electrospun Nanofibers in Biomedical Applications
- Diabetes Management and Research
- Advanced Cellulose Research Studies
- Liver physiology and pathology
- Inflammasome and immune disorders
- Blood Coagulation and Thrombosis Mechanisms
- Nerve injury and regeneration
- Cannabis and Cannabinoid Research
- Wound Healing and Treatments
- Hydrogels: synthesis, properties, applications
- Seaweed-derived Bioactive Compounds
- Polymer Surface Interaction Studies
- Atherosclerosis and Cardiovascular Diseases
- Proteoglycans and glycosaminoglycans research
- Clinical Nutrition and Gastroenterology
- Echinoderm biology and ecology
- Aquaculture disease management and microbiota
Norwegian University of Science and Technology
2015-2025
St Olav's University Hospital
2017-2025
Central Norway Regional Health Authority
2013-2017
National Center for Toxicological Research
2017
Institute of Fisheries and Aquaculture
1996
Alginate-poly-L-lysine (PLL) microcapsules can be used for transplantation of insulin-producing cells treatment type I diabetes. In this work we wanted to study the inflammatory reactions against implanted due PLL. We have seen that by reducing PLL layer, less overgrowth capsule is obtained. By incubating different cell types with and afterwards measuring viability MTT, found massive death at concentrations higher than 10 microg/ml. Staining annexin V propidium iodide showed induced necrosis...
Abstract One of the main challenges in tissue engineering and regenerative medicine is ability to maintain optimal cell function survival post‐transplantation. Biomaterials such as alginates are commonly used for immunoisolation, while they may also provide structural support transplants by mimicking extracellular matrix. In this study, arginine‐glycine‐aspartate (RGD)‐peptide‐coupled tailored composition were produced adopting a unique chemoenzymatic strategy substituting nongelling...
The structure of fucoidan isolated from Laminaria hyperborea was elucidated and chemically tailored in order to obtain a clear structure-function relationship on bioactive properties with minimal amount variations among the tested molecules. Analysis revealed sugar composition 97.8% fucose 2.2% galactose. glycosidic linkages showed (1→3)-α-l-fuco-pyranose (31.9%) be dominant residue, followed by 1→2-linked (13.2%) 1→4-linked (7.7%) fuco-pyranose as well high degree branching (22.4%)....
BackgroundDuring atherogenesis, cholesterol precipitates into crystals (CC) in the vessel wall, which trigger plaque inflammation by activating NACHT, LRR and PYD domains-containing protein 3 (NLRP3) inflammasome. We investigated relationship between CC, complement NLRP3 patients with cardiovascular disease.MethodsWe analysed plasma, peripheral blood mononuclear cells (PBMC) carotid plaques from advanced atherosclerosis applying ELISAs, multiplex cytokine assay, qPCR, immunohistochemistry,...
This study investigates the host response to fucoidan alginate microbeads in comparison sulfated microbeads, which are relevant for immune protection cell therapy. While reduce fibrosis and inflammation, fucoidan, a kelp-derived polysaccharide rich sulfate groups, has not been evaluated this context. The assesses surface reactivity acute-phase proteins cytokines using ex vivo human whole blood plasma models. It also examines pericapsular overgrowth (PFO) C57BL/6JRj mice, incorporating...
Lipedema is considered an inflammation-related disease, and low-carbohydrate ketogenic diets may help reduce inflammation. However, no randomized controlled trials have investigated the effect of a diet on inflammatory markers in females with lipedema. To compare changes fibrosis-associated after low-energy (LCD) compared low-fat lipedema, to explore potential associations between pain markers. Females lipedema obesity were randomly assigned either LCD or (both 1200 kcal/d) for 8 weeks. Body...
Microencapsulation of genetically engineered cells may have important applications as delivery systems for therapeutic proteins. However, optimization the microcapsules with regard to mechanical stability, cell growth, and secretion proteins is necessary in order evaluate future use this technology. We explored survival, from 293-EBNA producing endostatin (293 endo cells) JJN3 myeloma hepatocyte growth factor (HGF) that been embedded various types alginate capsules. Parameters affect capsule...
Alginate/poly-L-lysine(PLL)/alginate capsules are used widely for the microencapsulation of cells. Alginate consists guluronic acid and mannuronic acid, ratio sequence which affect properties alginate. Using C5-epimerases, can be converted to in alginate polymer. Such an enzyme, AlgE4, was convert blocks (M-blocks) alternating (MG-blocks). The aims this study were 1) investigate whether use epimerized as a coating could improve biocompatibility alginate/PLL/alginate 2) simple beads prepared...
Chronic inflammation of the arterial wall is a key element in development atherosclerosis, and cholesterol crystals (CC) that accumulate plaques are associated with initiation progression disease. We recently revealed link between complement system CC-induced inflammasome caspase-1 activation, showing trigger inflammation. HDL exhibits cardioprotective anti-inflammatory properties thought to explain its inverse correlation cardiovascular risk. In this study, we sought determine effect...
Iron oxide nanoparticles (IONPs) are promising nanomaterials for biomedical applications. However, their inflammatory potential has not been fully established. Here, we used a lepirudin anti-coagulated human whole blood model to evaluate the of 10 nm IONPs activate complement system and induce cytokine production. Reactive oxygen species cell death were also assessed. The activated complement, as measured by C3a, C5a sC5b-9, induced production pro-inflammatory cytokines in particle-dose...
Cholesterol crystals (CC) are strong activators of complement and could potentially be involved in thromboinflammation through complement-coagulation cross-talk. To explore the coagulation-inducing potential CC, we performed studies lepirudin-based human whole blood plasma models. In addition, immunohistological examinations brain thrombi vulnerable plaque material from patients with advanced carotid atherosclerosis were using polarization filter reflected light microscopy to identify CC....
Islet transplantation in diabetes is hampered by the need of life-long immunosuppression. Encapsulation provides partial immunoprotection but could possibly limit oxygen supply, a factor that may enhance hypoxia-induced beta cell death early posttransplantation period. Here we tested susceptibility alginate microencapsulated human islets to experimental hypoxia (0.1–0.3% O 2 for 8 h, followed reoxygenation) on viability and functional parameters. Hypoxia reduced as measured MTT by<mml:math...
Intra-peritoneal placement of alginate encapsulated human induced pluripotent stem cell-derived hepatocytes (hPSC-Heps) represents a potential new bridging therapy for acute liver failure. One the rate-limiting steps that needs to be overcome make such procedure more efficacious and safer is reduce accumulation fibrotic tissue around cells allow free passage relevant molecules in out metabolism. Novel chemical compositions afford possibility achieving this aim. We accordingly used sulfated...
The use of nonautologous cell lines producing a therapeutic substance encapsulated within alginate microcapsules could be an alternative way treating different diseases in cost-effective way. Malignant brain tumors have been proposed to treated locally using engineered cells secreting proteins with potential microcapsules. Optimization the capsule bioreactors is needed before this treatment can reality. Recently, we demonstrated that alginate-poly-L-lysine made high-G and gelled core...
Using a lepirudin-based human whole blood model, we evaluated the initial inflammatory and coagulation responses of dense porous ultrapure (<50 endotoxin units/grams) cellulose nanofibrils (CNF), carboxylated grade. The CNF was compared to wound dressing AquaCel because it is potential wound-healing material. aerogels induced strongest measured as prothrombin factor 1.2 (PTF1.2). complement response by terminal complex (TCC) surface C3c. All materials activated leukocytes CD11b, while levels...