A5036631657- Atherosclerosis and Cardiovascular Diseases
- Urologic and reproductive health conditions
- Prostate Cancer Diagnosis and Treatment
- Genomics, phytochemicals, and oxidative stress
- Prostate Cancer Treatment and Research
- Genital Health and Disease
- Adipokines, Inflammation, and Metabolic Diseases
- Bladder and Urothelial Cancer Treatments
- Sperm and Testicular Function
- Genetic factors in colorectal cancer
- Urinary and Genital Oncology Studies
- Cancer, Lipids, and Metabolism
- Antioxidant Activity and Oxidative Stress
- Urological Disorders and Treatments
- Systemic Lupus Erythematosus Research
- Cell Adhesion Molecules Research
- MRI in cancer diagnosis
- Autopsy Techniques and Outcomes
- Garlic and Onion Studies
- Cancer Genomics and Diagnostics
- Nutrition, Genetics, and Disease
- Lipoproteins and Cardiovascular Health
- Renal cell carcinoma treatment
- Colorectal Cancer Screening and Detection
- PI3K/AKT/mTOR signaling in cancer
Norfolk and Norwich University Hospitals NHS Foundation Trust
2011-2024
Norfolk and Norwich University Hospital
2005-2022
Norwich Research Park
2017
University of Cambridge
1977-2009
Cambridge University Hospitals NHS Foundation Trust
2006
University of Medicine and Pharmacy of Craiova
2006
Temple University Hospital
2006
University of Pittsburgh
2006
Norfolk Community Health and Care NHS Trust
1995-2001
Norwich University
2001
Abstract The adamalysin-thrombospondin (ADAMTS) proteinases are a relatively newly described branch of the metzincin family that contain metalloproteinase, disintegrin, and thrombospondin motifs. They have been implicated in various cellular events, including cleavage proteoglycans, extracellular matrix degradation, inhibition angiogenesis, gonadal development, organogenesis. However, many cases, their normal physiological roles potential for dysregulation malignancy remain to be...
Extracellular proteases of the matrix metalloproteinase (MMP) and serine protease families participate in many aspects tumour growth metastasis. Using quantitative real-time RT–PCR analysis, we have undertaken a comprehensive survey expression these enzymes their natural inhibitors 44 cases human prostate cancer 23 benign specimens. We found increased MMP10, 15, 24, 25 26, urokinase plasminogen activator-receptor (uPAR) activator inhibitor-1 (PAI1), newly characterised hepsin matriptase-1...
Sections of human atherosclerotic plaques were stained by the indirect immunoperoxidase technique using three rat anti-human monoclonal antibodies: YAML 501.4 (anti-'leukocyte-common' (T200) antigen; YTH 8.18 (antimacrophage cytoplasm); and YPC 1/3 . 12 (anti-smooth muscle cell). The cells diffuse intimal thickening almost all smooth but there rare subendothelial macrophages. Focal lesions, in contrast, contained numerous macrophages as well cells. Macrophage 'foam cells' particularly fatty...
BackgroundEpidemiological studies suggest that people who consume more than one portion of cruciferous vegetables per week are at lower risk both the incidence prostate cancer and developing aggressive but there is little understanding underlying mechanisms. In this study, we quantify interpret changes in global gene expression patterns human gland before, during after a 12 month broccoli-rich diet.Methods FindingsVolunteers were randomly assigned to either or pea-rich diet. After six months...
Epidemiological evidence suggests that consumption of cruciferous vegetables is associated with reduced risk prostate cancer progression, largely attributed to the biological activity glucosinolate degradation products, such as sulforaphane derived from glucoraphanin. Because there are few therapeutic interventions for men on active surveillance reduce dietary approaches an appealing option patients.We evaluated whether a glucoraphanin-rich broccoli soup 1 y leads changes in gene expression...
The frequency of prostatic core biopsies to detect cancer has been increasing with more widespread prostate specific antigen (PSA) testing. Gleason score important implications for patient management but morphological reproducibility data British practice are limited. Using literature-based criteria nine uropathologists took part in a study.Each the participants submitted slides from consecutive cases biopsy-diagnosed assigned groups 2-4, 5-6, 7 and 8-10 original report. A random selection...
Variations in surgical technique of temporal artery biopsy (TAB) performed for diagnosis giant cell arteritis (GCA) may contribute to high false-negative rates. This was a retrospective analysis large database that explored potential associations between specimen length and diagnostic sensitivity TAB.Histopathological reports medical records patients who underwent TAB six hospitals 2004 2009 were reviewed.A total 966 biopsies analysed. The median postfixation 1 (range 0·1-8·5) cm 207 (21·4...
Abstract Background The PTEN tumour suppressor gene and PIK3CA proto-oncogene encode proteins which contribute to regulation propagation of signal transduction through the PI3K/AKT signalling pathway. This study investigates prevalence loss expression mutations in both colorectal cancers (CRC) their associations with clinicopathological features, lifestyle factors dietary consumptions. Methods 186 adenocarcinomas 16 adenomas from EPIC Norfolk were tested for by DNA sequencing changes...
Summary Fifteen epididymectomies were performed on 10 patients with post‐vasectomy pain and 12 specimens available for histopathological review. The findings compared those in 2 groups which epididymectomy was chronic epididymo‐orchitis epididymal cysts. results showed that 50% of the group cured by simple epididymectomy. Pathological revealed features long‐standing obstruction interstitial perineural fibrosis may have accounted pain. It is important to recognise this late complication...
Abstract Background Dietary or therapeutic interventions to counteract the loss of PTEN expression could contribute prevention prostate carcinogenesis reduce rate cancer progression. In this study, we investigate interaction between sulforaphane, a dietary isothiocyanate derived from broccoli, and gene in pre malignant tissue. Results We initially describe heterogeneity non-malignant tissue men deemed be at risk cancer. subsequently use mouse prostate-specific deletion model, show that...
To develop a risk classifier using urine-derived extracellular vesicle (EV)-RNA capable of providing diagnostic information on disease status prior to biopsy, and prognostic for men active surveillance (AS).Post-digital rectal examination EV-RNA expression profiles (n = 535, multiple centres) were interrogated with curated NanoString panel. A LASSO-based continuation ratio model was built generate four prostate urine (PUR) signatures predicting the probability normal tissue (PUR-1), D'Amico...
N-acetylneuraminic acid at the surfaces of rat cerebral cortex and liver mitochondria derived mitoplasts (inner membrane plus matrix particles) was studied biochemically electrokinetically. Rat in 0.0145 M NaCl, 4.5% sorbitol, pH 7.2 ± 0.1, 0.6 mM NaHCO3, had an electrophoretic mobility - 2.88 0.01 µ/sec per v cm. In same solution 2.01 0.02, 1.22 0.07, 0.91 0.04 Treatment these particles with 50 µg neuraminidase/mg particle protein resulted following mobilities cm: mitochondria, 2.27; 1.40;...
Diets rich in cruciferous vegetables have been associated with a lower risk of incidence and progression prostate cancer. Sulforaphane, an isothiocyanate derived from 4-methylsulphinylbutyl glucosinolate (glucoraphanin) that accumulates certain these vegetables, notably broccoli, has implicated their protective effects. Likewise, the consumption garlic its sulphur-containing compounds such as alliin reduction In this study, we tested whether consuming glucoraphanin broccoli seeds resulted...
Abstract Testicular germ cell tumours (TGCT), which comprise seminoma and non-seminoma subtypes, are the most common cancers in young men. In this study, we present a comprehensive whole genome sequencing analysis of adult TGCTs. Leveraging samples from participants recruited via UK National Health Service data Genomics England 100,000 Genomes Project, our results provide an extended description genomic elements underlying TGCT pathogenesis. This catalogue offers comprehensive,...
To test the effectiveness of a teaching resource (a decision tree with diagnostic criteria based on published literature) in improving proficiency Gleason grading prostatic cancer by general pathologists.A was developed panel urological pathologists during reproducibility study. Twenty-four histopathologists tested this resource. Twenty slides were selected to include range score groups 2-4, 5-6, 7 and 8-10. Interobserver agreement studied before after presentation criteria. The results...
Abstract There is conflicting evidence for the role diet and lifestyle play in development of mismatch repair (MMR)-deficient colorectal cancers (CRC). In this study, associations between MMR deficiency, clinicopathological characteristics, dietary factors sporadic CRC were investigated. Tumor samples from 185 individuals EPIC-Norfolk study analyzed MLH1 gene promoter methylation microsatellite instability (MSI). Dietary data collected prospectively using 7-day food diaries (7dd)...