A5090165896- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Plant Micronutrient Interactions and Effects
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Liver Disease Diagnosis and Treatment
- Plant Virus Research Studies
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Agronomic Practices and Intercropping Systems
- Soybean genetics and cultivation
- Mycorrhizal Fungi and Plant Interactions
- Diet, Metabolism, and Disease
- Fatty Acid Research and Health
- Plant and animal studies
- Peroxisome Proliferator-Activated Receptors
- Plant biochemistry and biosynthesis
- Photosynthetic Processes and Mechanisms
- Aluminum toxicity and tolerance in plants and animals
- Insect-Plant Interactions and Control
- Plant Physiology and Cultivation Studies
- Cocoa and Sweet Potato Agronomy
- Plant Parasitism and Resistance
- Diet and metabolism studies
- Plant-Microbe Interactions and Immunity
West Virginia University
2016-2025
West Virginia State University
2020-2023
Universidad Autónoma de Coahuila
2023
Virginia State University
2023
The University of Texas Rio Grande Valley
2023
Plant (United States)
2023
Huazhong Agricultural University
2020
Anhui Agricultural University
2020
Noble Research Institute
2006-2017
John Innes Centre
2007-2016
Legumes (Fabaceae or Leguminosae) are unique among cultivated plants for their ability to carry out endosymbiotic nitrogen fixation with rhizobial bacteria, a process that takes place in specialized structure known as the nodule. belong one of two main groups eurosids, Fabidae, which includes most species capable fixation. comprise several evolutionary lineages derived from common ancestor 60 million years ago (Myr ago). Papilionoids largest clade, dating nearly origin legumes and containing...
Legumes played central roles in the development of agriculture and civilization, today account for approximately one-third world's primary crop production. Unfortunately, most cultivated legumes are poor model systems genomic research. Therefore, Medicago truncatula, which has a relatively small diploid genome, been adopted as species legume genomics. To enhance its value model, we have generated gene expression atlas that provides global view all major organ this species, with special...
During arbuscular mycorrhizal (AM) symbiosis, the plant gains access to phosphate (Pi) and nitrogen delivered by its fungal symbiont. Transfer of mineral nutrients occurs at interface between branched hyphae called arbuscules root cortical cells. In Medicago truncatula, a Pi transporter, PT4, is required for symbiotic transport, in pt4, transport fails, degenerate prematurely, symbiosis not maintained. Premature arbuscule degeneration (PAD) suppressed when pt4 mutants are nitrogen-deprived,...
Abstract Background The tomato ( Solanum lycopersicum L.) plant is both an economically important food crop and ideal dicot model to investigate various physiological phenomena not possible in Arabidopsis thaliana . Due the great diversity of cultivars used by research community, it often difficult reliably compare phenotypes. lack developmental mutants a single genetic background prevents stacking mutations facilitate analysis double multiple mutants, required for elucidating pathways....
Legumes (Leguminosae or Fabaceae) play a major role in agriculture. Transcriptomics studies the model legume species, Medicago truncatula, are instrumental helping to formulate hypotheses about of genes. With rapid growth publically available Affymetrix GeneChip Genome Array data from great range tissues, cell types, conditions, and stress treatments, research community desires an effective bioinformatics system aid efforts interpret genome through functional genomics. We developed...
Given the susceptibility of tomato plants to pests, aim present study was understand how hormones are involved in formation natural defences against insect herbivory. Tomato hormone mutants, previously introgressed into same genetic background reference, were screened for alterations trichome densities and allelochemical content. Ethylene, gibberellin, auxin mutants indirectly showed alteration density, through effects on epidermal cell area. However, brassinosteroids (BRs) jasmonates (JAs)...
World population is projected to reach its maximum (~10 billion people) by the year 2050. This 45% increase of current world (approaching seven will boost demand for food and raw materials. However, we live in a historical moment when supply phosphate, water, oil are at their peaks. Modern agriculture fundamentally based on varieties bred high performance under input systems (fertilizers, oil, pesticides), which generally do not perform well low-input situations. We propose shift research...
Optimization of nitrogen fixation by rhizobia in legumes is a key area research for sustainable agriculture. Symbiotic (SNF) occurs specialized organs called nodules and depends on steady supply carbon to both plant bacterial cells. Here we report the functional characterization nodule-specific Suc transporter, MtSWEET11 from Medicago truncatula belongs clade SWEET proteins that are capable transporting play critical roles pathogen susceptibility. When expressed mammalian cells, transported...
Transcription factors (TFs) are thought to regulate many aspects of nodule and symbiosis development in legumes, although few TFs have been characterized functionally. Here, we describe regulator symbiosome differentiation (RSD) Medicago truncatula, a member the Cysteine-2/Histidine-2 (C2H2) family plant that is required for normal during development. RSD expressed nodule-specific manner, with maximal transcript levels bacterial invasion zone. A tobacco (Nicotiana tabacum) retrotransposon...
We are at a pivotal time in human history, as the agricultural sector undergoes consolidation coupled with increasing energy costs context of declining resource availability. Although organic systems often thought more sustainable than conventional operations, lack concise and widely accepted means to measure sustainability makes coming an agreement on this issue quite challenging. However, accurate assessment can be reached by dissecting scientific underpinnings opposing production...
Summary The multidrug and toxin extrusion ( MATE ) transporter family comprises 70 members in the Medicago truncatula genome, they play seemingly important, yet mostly uncharacterized, physiological functions. Here, we employed bioinformatics molecular genetics to identify characterize transporters involved citric acid export, Al 3+ tolerance Fe translocation. Mt 69 is a induced by Fe‐deficiency. Overexpression of hairy roots altered homeostasis hormone levels under Fe‐deficient or...
Abstract A Tnt1-insertion mutant population of Medicago truncatula ecotype R108 was screened for defects in nodulation and symbiotic nitrogen fixation. Primary screening 9,300 lines yielded 317 with putative nodule development and/or Of these, 230 were rescreened, 156 confirmed defective Mutants sorted into six distinct phenotypic categories: 72 nonnodulating mutants (Nod−), 51 totally ineffective nodules (Nod+ Fix−), 17 partially Fix+/−), 27 emergence, elongation, fixation (Nod+/− one...
Species in the Solanaceae family are known for producing plethora of specialized metabolites. In addition to biosynthesis pathways, a full comprehension secondary metabolism must also take into account transport and subcellular compartmentalization substances. Here, we examined MATE (Multidrug Toxic Compound Extrusion, or Multi-Antimicrobial Extrusion) gene tomato (Solanum lycopersicum) genome with objective better understanding metabolites this model species. membrane effluxers encompass an...
Iron (Fe) is an essential micronutrient for symbiotic nitrogen fixation in legume nodules, where it required the activity of bacterial nitrogenase, plant leghemoglobin, respiratory oxidases, and other Fe proteins both organisms. solubility transport within between tissues facilitated by organic chelators, such as nicotianamine citrate. We have characterized a nodule-specific citrate transporter multidrug toxic compound extrusion family, MtMATE67
Abstract Type-IV glandular trichomes, which only occur in the juvenile developmental phase of cultivated tomato (Solanum lycopersicum), produce acylsugars that broadly protect against arthropod herbivory. Previously, we introgressed capacity to retain type-IV trichomes adult from wild tomato, Solanum galapagense, into species cv. Micro-Tom (MT). The resulting MT-Galapagos enhanced trichome (MT-Get) introgression line contained 5 loci associated with enhancing density plants. We genetically...
Transporters move hydrophilic substrates across hydrophobic biological membranes and play key roles in plant nutrition, metabolism, signaling and, consequently, growth, development, responses to the environment. To initiate support systematic characterization of transporters model legume Medicago truncatula, we identified 3,830 classified 2,673 these into 113 families 146 subfamilies. Analysis gene expression data for 2,611 129 that are expressed an organ-specific manner, including 50 nodule...
Numerous health benefits associated with increased omega-3 polyunsaturated fatty acid (n-3 PUFA) consumption has lead to an increasing variety of available n-3 PUFA sources. However, sources differ in the type, amount, and structural form PUFAs. Therefore, objective this study was determine effect different ω-3 PUFAs on digestibility, tissue deposition, eicosanoid metabolism, oxidative stability. Female Sprague-Dawley rats (age 28 d) were randomly assigned (n = 10/group) be fed a high fat...
Building accurate gene regulatory networks (GRNs) from high-throughput expression data is a long-standing challenge. However, with the emergence of new algorithms combined increase transcriptomic availability, it now reachable. To help biologists to investigate relationships, we developed web-based computational service build, analyze and visualize GRNs that govern various biological processes. The web server preloaded all available Affymetrix GeneChip-based annotation three model legume...